FastQCFastQC Report
Fri 10 Feb 2017
ERR1631118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647826
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT207073.1963829793802656No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG164532.5397251731174726No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT144402.228993587784374TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC89561.382469984224159No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC56540.8727652178208346RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51960.8020672217539895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.775671245056543No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC45660.7048188865528706No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG20910.32277185540561815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19760.30502017517049335No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17650.27244970099995985No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA16110.24867788572857527No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT12850.19835573132291695No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11420.17628190285663126No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10970.1693355931994085No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9770.15081210078014776No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9600.14818793935408583No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8720.13460404491329464No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8090.12487921139318274No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7800.12040270072519471No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7460.11515437787307085No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7250.11191276669970023No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7170.11067786720508285No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6920.10681880628440352No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6920.10681880628440352No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6820.1052751819161318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.10280538292689705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG200.001841342937.029
CTTATAC24000.033.531251
TTATACA24100.033.3921172
CGTCTTC6650.032.8270737
GATTACG352.3867115E-531.7142851
TATACAC26000.031.3076933
GGTATCA7300.030.9178071
TCGCATG250.00549459429.637
TAGACCC250.00549459429.64
ATTCGAC250.00549459429.633
GCCATTT250.00549459429.633
CCTTTAT502.7176793E-729.61
TCGACGG250.00549459429.635
CGCCTAT454.004707E-628.77777934
GGACCCG358.8652014E-426.4285721
CCAAAAC358.8652014E-426.4285720
TGTGGAT509.083091E-625.90000211
CCGTCTT20700.025.02415537
CTTATAT451.3223411E-424.66666832
CCACATA451.3223411E-424.66666813
Produced by FastQC (version 0.11.5)