##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647826
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404533007319866	31.0	31.0	34.0	28.0	34.0
2	31.40279797352993	31.0	31.0	34.0	28.0	34.0
3	30.864005149530893	31.0	31.0	34.0	27.0	34.0
4	35.02836101051825	37.0	35.0	37.0	32.0	37.0
5	35.23510942753147	37.0	35.0	37.0	33.0	37.0
6	35.44534180474387	37.0	35.0	37.0	33.0	37.0
7	35.44360522732956	37.0	35.0	37.0	33.0	37.0
8	35.473171808479435	37.0	35.0	37.0	33.0	37.0
9	37.24960869122264	39.0	37.0	39.0	34.0	39.0
10	37.07918947371671	39.0	37.0	39.0	33.0	39.0
11	37.13886444816973	39.0	37.0	39.0	33.0	39.0
12	37.056354638436865	39.0	37.0	39.0	33.0	39.0
13	37.157429618446926	39.0	37.0	39.0	33.0	39.0
14	38.173893915958914	40.0	37.0	41.0	33.0	41.0
15	38.1868017029264	40.0	37.0	41.0	33.0	41.0
16	38.18205042712086	40.0	38.0	41.0	33.0	41.0
17	38.18509599799946	40.0	38.0	41.0	33.0	41.0
18	38.256244732381845	40.0	38.0	41.0	33.0	41.0
19	38.235685199420836	40.0	38.0	41.0	34.0	41.0
20	38.15927116231828	40.0	37.0	41.0	33.0	41.0
21	38.14614418069049	40.0	37.0	41.0	33.0	41.0
22	38.07324651989886	40.0	37.0	41.0	33.0	41.0
23	38.07282819769506	40.0	37.0	41.0	33.0	41.0
24	38.04329248903255	40.0	37.0	41.0	33.0	41.0
25	37.94010428726232	40.0	37.0	41.0	33.0	41.0
26	37.82766514465304	40.0	37.0	41.0	33.0	41.0
27	37.681133514246106	39.0	37.0	41.0	32.0	41.0
28	37.7031147252503	40.0	37.0	41.0	33.0	41.0
29	37.67346015751143	39.0	37.0	41.0	33.0	41.0
30	37.41159354518034	39.0	36.0	41.0	32.0	41.0
31	37.40357596021154	39.0	36.0	41.0	32.0	41.0
32	37.301301584067325	39.0	36.0	41.0	32.0	41.0
33	37.192422039251284	39.0	36.0	41.0	31.0	41.0
34	37.18217082982159	39.0	36.0	41.0	31.0	41.0
35	37.136897870724546	39.0	36.0	41.0	31.0	41.0
36	37.06483222346741	39.0	36.0	41.0	31.0	41.0
37	37.004516644901564	39.0	35.0	41.0	31.0	41.0
38	36.954021604566655	39.0	35.0	41.0	31.0	41.0
39	36.82584058064974	39.0	35.0	41.0	31.0	41.0
40	36.643597200482844	39.0	35.0	40.0	30.0	41.0
41	36.59168202572913	39.0	35.0	40.0	30.0	41.0
42	36.4869563740881	39.0	35.0	40.0	30.0	41.0
43	35.72983949393819	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	11.0
10	13.0
11	16.0
12	18.0
13	17.0
14	13.0
15	7.0
16	10.0
17	15.0
18	23.0
19	44.0
20	86.0
21	170.0
22	338.0
23	603.0
24	1072.0
25	1751.0
26	2902.0
27	4418.0
28	6439.0
29	9147.0
30	12202.0
31	15688.0
32	20380.0
33	26442.0
34	34480.0
35	46421.0
36	64117.0
37	95526.0
38	154134.0
39	151320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.90960226974527	22.14329156285793	16.07514980874494	24.87195635865186
2	13.935840796757153	21.41639884783877	41.52457604356725	23.12318431183682
3	13.807874336627426	30.0894067234103	34.22493076844708	21.877788171515192
4	10.532920876902132	19.832331521118324	36.214353854275686	33.420393747703855
5	12.35655870557835	37.97701852040517	34.657299953999996	15.009122820016485
6	26.322345815079974	40.69796519435774	16.514002216644595	16.46568677391769
7	23.794969636908675	32.731011104833705	21.22082164037874	22.253197617878875
8	25.525063828867626	32.78179634654985	22.37298286885676	19.320156955725764
9	23.29946621469345	13.501927986835971	17.937223884191127	45.261381914279454
10	15.650498745033389	27.47080234507414	31.552608262095067	25.3260906477974
11	30.670581298064604	22.63400974953151	24.299117355586223	22.39629159681766
12	20.561076585379407	27.99856751658624	25.09794296616684	26.34241293186751
13	30.80611151759886	19.20639183978414	25.826688030427924	24.160808612189076
14	22.725546674570023	22.61934531803293	25.18947989120535	29.465628116191695
15	25.456526906916366	27.61358759913928	21.94107677061433	24.98880872333003
16	25.94693636871629	24.971674492842215	22.252580168131566	26.828808970309932
17	20.593801421986768	31.094460549592018	22.6935936501468	25.618144378274415
18	25.840117562431885	22.52749966812076	24.780573796050174	26.85180897339718
19	26.679849218771707	25.02107047262691	26.04881557702223	22.250264731579158
20	22.544942623482232	23.861962934491668	27.67162787538629	25.921466566639808
21	26.271406210927005	25.862191390898175	24.87195635865186	22.99444603952296
22	22.728788285743395	31.020520942351805	25.840271924868713	20.410418847036087
23	24.358701256201513	25.750896073945782	25.724808822121993	24.16559384773072
24	28.25264808760377	26.74143983106575	24.520627452433217	20.485284628897265
25	23.103889007233423	28.82950051402691	26.740513656444787	21.326096822294875
26	27.173037204434525	28.5726414191465	24.85111742968019	19.403203946738785
27	22.25072781888964	29.00454751738893	28.631144782704	20.113579881017436
28	19.963385229984596	27.908419853479177	28.425379654413373	23.702815262122854
29	28.705393114817866	23.91815086149676	25.946782006279463	21.429674017405908
30	21.699036469669323	26.369889445622746	31.436218984727383	20.49485509998055
31	25.479526910003614	27.768721848150584	24.63485565568532	22.11689558616048
32	27.48222516539935	24.620963036370878	27.148956664289486	20.747855133940288
33	20.040257723524526	29.647312704337274	29.443091200414926	20.86933837172327
34	24.17639921830862	27.118238539360878	26.97221167412237	21.733150568208128
35	24.32427843278905	23.699573650949482	32.08454121940151	19.89160669685996
36	23.46170113579881	27.926634621024782	24.00119785250978	24.61046639066663
37	21.52136530488125	26.11411088780012	28.745990435703415	23.618533371615218
38	21.877633809078365	23.291285005541614	29.167091163367942	25.663990022012083
39	24.720526808124404	23.96121798137154	27.092151287537085	24.226103922966967
40	20.18798257556813	23.174587003300267	34.96633355252799	21.671096868603605
41	22.86123125654111	24.142439482206644	25.80337930246702	27.192949958785228
42	22.520090271153055	23.872613942632746	31.146326328365948	22.460969457848247
43	23.394244750905337	22.048821751519696	30.9847088570079	23.572224640567065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	35.0
2	28.0
3	123.5
4	219.0
5	219.0
6	328.0
7	437.0
8	435.5
9	434.0
10	700.5
11	967.0
12	967.0
13	1472.5
14	1978.0
15	4753.0
16	7528.0
17	7750.5
18	7973.0
19	7973.0
20	6396.0
21	4819.0
22	3999.5
23	3180.0
24	3667.0
25	4154.0
26	4154.0
27	5258.0
28	6362.0
29	7947.0
30	9532.0
31	12388.0
32	15244.0
33	15244.0
34	19339.0
35	23434.0
36	25942.0
37	28450.0
38	33658.0
39	38866.0
40	38866.0
41	41792.5
42	44719.0
43	47392.5
44	50066.0
45	52029.5
46	53993.0
47	53993.0
48	56391.5
49	58790.0
50	73723.0
51	88656.0
52	67210.5
53	45765.0
54	45765.0
55	56391.0
56	67017.0
57	53826.0
58	40635.0
59	29325.0
60	18015.0
61	18015.0
62	15040.5
63	12066.0
64	9188.5
65	6311.0
66	4923.5
67	3536.0
68	3536.0
69	2807.0
70	2078.0
71	1643.5
72	1209.0
73	842.0
74	475.0
75	475.0
76	366.5
77	258.0
78	217.0
79	176.0
80	127.5
81	79.0
82	79.0
83	86.0
84	93.0
85	111.0
86	129.0
87	100.0
88	71.0
89	71.0
90	54.5
91	38.0
92	20.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	647826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.912878365492094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.39178162751803	16.816169654580783
2	10.953406754129274	5.019481532902473
3	3.8772714186811696	2.6651834521872186
4	2.0564522821073945	1.8847696401746143
5	1.323279033647372	1.516006577078408
6	1.0040349836326457	1.3803198872804194
7	0.7428045553967795	1.1913853298997892
8	0.612198897002476	1.1221791090004924
9	0.48627664546713223	1.0027797866611132
>10	4.398966629136661	20.901087882670506
>50	0.6864131678558766	10.9340971538665
>100	0.43995621441207516	18.640359719826787
>500	0.016973619383181916	2.6197279126869057
>1k	0.0054315582026182125	2.4164034244516452
>5k	0.0027157791013091063	3.8628541448888694
>10k+	0.0020368343259818296	8.027194791843488
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	20707	3.1963829793802656	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	16453	2.5397251731174726	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	14440	2.228993587784374	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	8956	1.382469984224159	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	5654	0.8727652178208346	RNA PCR Primer, Index 3 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5196	0.8020672217539895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5025	0.775671245056543	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	4566	0.7048188865528706	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	2091	0.32277185540561815	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.30502017517049335	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1765	0.27244970099995985	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1611	0.24867788572857527	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	1285	0.19835573132291695	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1142	0.17628190285663126	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	1097	0.1693355931994085	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	977	0.15081210078014776	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	960	0.14818793935408583	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	872	0.13460404491329464	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	809	0.12487921139318274	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	780	0.12040270072519471	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	746	0.11515437787307085	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	725	0.11191276669970023	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	717	0.11067786720508285	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	692	0.10681880628440352	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	692	0.10681880628440352	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	682	0.1052751819161318	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	666	0.10280538292689705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.630873104815182E-4	0.0
11	0.0	0.0	0.0	9.261746209630364E-4	0.0
12	0.0	0.0	0.0	0.0010805370577902092	0.0
13	0.0	0.0	0.0	0.0010805370577902092	0.0
14	0.0	0.0	0.0	0.0010805370577902092	0.0
15	0.0	0.0	0.0	0.0010805370577902092	0.0
16	0.0	0.0	0.0	0.0012348994946173818	0.0
17	0.0	0.0	0.0	0.0012348994946173818	0.0
18	0.0	0.0	0.0	0.0015436243682717273	0.0
19	0.0	0.0	0.0	0.0015436243682717273	0.0
20	0.0	0.0	0.0	0.0018523492419260728	0.0
21	0.0	0.0	0.0	0.0027785238628891093	0.0
22	0.0	0.0	0.0	0.0033959736101978	0.0
23	0.0	0.0	0.0	0.0050939604152967	0.0
24	0.0	0.0	0.0	0.006946309657222773	0.0
25	0.0	0.0	0.0	0.007409396967704291	0.0
26	0.0	0.0	0.0	0.008026846715012982	0.0
27	0.0	0.0	0.0	0.008644296462321673	0.0
28	0.0	0.0	0.0	0.01914094216656942	0.0
29	0.0	0.0	0.0	0.04461074424305292	0.0
30	0.0	0.0	0.0	0.07455705698752443	0.0
31	0.0	0.0	0.0	0.13136243373992398	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	20	0.0018413429	37.0	29
CTTATAC	2400	0.0	33.53125	1
TTATACA	2410	0.0	33.392117	2
CGTCTTC	665	0.0	32.82707	37
GATTACG	35	2.3867115E-5	31.714285	1
TATACAC	2600	0.0	31.307693	3
GGTATCA	730	0.0	30.917807	1
TCGCATG	25	0.005494594	29.6	37
TAGACCC	25	0.005494594	29.6	4
ATTCGAC	25	0.005494594	29.6	33
GCCATTT	25	0.005494594	29.6	33
CCTTTAT	50	2.7176793E-7	29.6	1
TCGACGG	25	0.005494594	29.6	35
CGCCTAT	45	4.004707E-6	28.777779	34
GGACCCG	35	8.8652014E-4	26.42857	21
CCAAAAC	35	8.8652014E-4	26.42857	20
TGTGGAT	50	9.083091E-6	25.900002	11
CCGTCTT	2070	0.0	25.024155	37
CTTATAT	45	1.3223411E-4	24.666668	32
CCACATA	45	1.3223411E-4	24.666668	13
>>END_MODULE