FastQCFastQC Report
Fri 10 Feb 2017
ERR1631117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121856
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT79000.7041901990986366No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG62680.5587169832848422No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT51760.4613782874094358RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48050.4283080894517657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.415115665468652No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA39560.3526299275486337No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC35430.3158159335957556No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC18320.16330081579097494RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC17890.15946788179588112No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17810.1587547777967939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16210.14449269781504936No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA11550.10295438986821838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGAG251.2336067E-437.035
CGTTACG250.005496287729.616
GGTATCA7800.029.4102541
GATTACG950.029.2105271
CTTATAC11700.028.461541
GTATCAA25200.024.9603161
TATACAC14350.024.6236933
TTATACA14100.024.1418442
CGTCTTC3450.024.13043637
CCGTCTT9300.023.27419337
CGGCATT400.001931158523.12527
TACGTTA400.001931158523.12519
GCCGTCT9400.022.82978636
GCTTTAT8750.021.7771431
TTACGGG1400.021.1428573
ACGTCCT450.003825751620.55555535
GATAGCG450.003825751620.5555552
CGCTTAA450.003825751620.5555551
CGCCGTT555.1429874E-420.18181825
AGGACCG656.90194E-519.9230775