##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631117.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1121856
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472798647954818	31.0	31.0	34.0	28.0	34.0
2	31.45136719864225	31.0	31.0	34.0	28.0	34.0
3	30.91931228250328	31.0	31.0	34.0	27.0	34.0
4	35.08492622939129	37.0	35.0	37.0	32.0	37.0
5	35.2935144974043	37.0	35.0	37.0	33.0	37.0
6	35.487785419875635	37.0	35.0	37.0	33.0	37.0
7	35.49806124850248	37.0	35.0	37.0	33.0	37.0
8	35.52166677363227	37.0	35.0	37.0	33.0	37.0
9	37.274463924068684	39.0	37.0	39.0	34.0	39.0
10	37.1211724142849	39.0	37.0	39.0	33.0	39.0
11	37.17618125677449	39.0	37.0	39.0	33.0	39.0
12	37.118580281248214	39.0	37.0	39.0	33.0	39.0
13	37.189616136117294	39.0	37.0	39.0	33.0	39.0
14	38.32933460265845	40.0	38.0	41.0	34.0	41.0
15	38.33529258657082	40.0	38.0	41.0	34.0	41.0
16	38.338425787266814	40.0	38.0	41.0	34.0	41.0
17	38.341443108562956	40.0	38.0	41.0	34.0	41.0
18	38.360105931599065	40.0	38.0	41.0	34.0	41.0
19	38.398001169490556	40.0	38.0	41.0	34.0	41.0
20	38.39584313851332	40.0	38.0	41.0	34.0	41.0
21	38.355509976324946	40.0	38.0	41.0	34.0	41.0
22	38.293426250784414	40.0	38.0	41.0	34.0	41.0
23	38.276181613326486	40.0	38.0	41.0	34.0	41.0
24	38.2524236622169	40.0	38.0	41.0	34.0	41.0
25	38.22372657453363	40.0	38.0	41.0	34.0	41.0
26	38.120755248445434	40.0	37.0	41.0	33.0	41.0
27	38.06938680187118	40.0	37.0	41.0	33.0	41.0
28	38.0253900678875	40.0	37.0	41.0	33.0	41.0
29	37.95821210565349	40.0	37.0	41.0	33.0	41.0
30	37.875924361058814	40.0	37.0	41.0	33.0	41.0
31	37.838763620286386	40.0	37.0	41.0	33.0	41.0
32	37.763566803582634	40.0	37.0	41.0	33.0	41.0
33	37.73922945547378	40.0	37.0	41.0	33.0	41.0
34	37.67856213275144	40.0	37.0	41.0	33.0	41.0
35	37.63129581693194	40.0	37.0	41.0	33.0	41.0
36	37.59186116578242	40.0	37.0	41.0	33.0	41.0
37	37.53996502224884	40.0	37.0	41.0	32.0	41.0
38	37.49235106822979	40.0	37.0	41.0	32.0	41.0
39	37.40839287751726	39.0	37.0	41.0	32.0	41.0
40	37.34842350533402	39.0	36.0	41.0	32.0	41.0
41	37.28690402333276	39.0	36.0	41.0	31.0	41.0
42	37.22285569627474	39.0	36.0	41.0	31.0	41.0
43	36.62002342546637	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	5.0
11	11.0
12	7.0
13	5.0
14	3.0
15	6.0
16	4.0
17	12.0
18	22.0
19	44.0
20	80.0
21	183.0
22	348.0
23	708.0
24	1253.0
25	2365.0
26	3833.0
27	5809.0
28	8908.0
29	12930.0
30	17884.0
31	24078.0
32	31158.0
33	41442.0
34	54783.0
35	74045.0
36	103539.0
37	155804.0
38	271393.0
39	311188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.858145786981574	21.99658423184437	15.246163500484911	22.899106480689145
2	14.034243254036168	22.97496291859205	40.29982457641623	22.690969250955558
3	15.036065234753837	28.440281105596437	33.98047521250499	22.543178447144733
4	11.785202378914942	18.735470506018597	40.44030606423641	29.039021050830055
5	12.526741399965772	38.44370400479205	36.00934522790804	13.020209367334132
6	27.514048148782017	41.29674396714017	17.776791317245706	13.412416566832105
7	24.792486736265616	33.02402447372925	24.0242954532489	18.159193336756232
8	24.975754464031034	34.91900921330367	22.189568001597355	17.915668321067947
9	25.040736065947854	15.481755234183353	20.840910063323633	38.63659863654515
10	14.695736351189456	29.285309344514804	34.01720006845798	22.001754235837755
11	32.091997546922244	24.371131410804953	25.223914655713394	18.312956386559414
12	21.663653802270524	26.61125848593759	30.524594956928517	21.20049275486337
13	28.537708939471734	22.075203947743738	26.7711720577329	22.615915055051627
14	21.3368738946888	21.963068343887272	28.96681927092247	27.733238490501456
15	23.14370115237606	29.803825089851106	24.48255391066233	22.569919847110505
16	22.5060970391922	28.50419305151463	25.503718837355237	23.48599107193793
17	21.67943122825033	28.92795510297222	26.67935991785042	22.713253750927034
18	22.930839608648526	26.839808317645048	28.130972245992353	22.098379827714073
19	24.203373695019682	26.779729305721943	27.777005248445434	21.23989175081294
20	23.717215043641964	26.517752724057274	27.4977358948029	22.26729633749786
21	23.380362542073136	26.67668577785384	28.06991271607051	21.87303896400251
22	21.626572394317986	29.355995778424326	27.958401078213246	21.059030749044442
23	22.563323635118945	26.946684779508246	28.030424582121054	22.459567003251752
24	22.37524245535969	27.630908066632436	27.741260910491185	22.25258856751669
25	21.810464088082608	28.674268355296938	27.597570454675107	21.917697101945347
26	22.993236208568657	27.240572765132065	27.621192024644873	22.1449990016544
27	21.907981059957784	28.28535926179474	27.742954532489016	22.06370514575846
28	21.71749315420161	27.383193564949508	28.590211221404534	22.30910205944435
29	22.28877859547036	28.069645302070857	27.397544782931142	22.24403131952764
30	22.158458839637174	27.860973244337956	27.838599606366593	22.14196830965828
31	22.845534542757715	26.900956985566776	28.032029066119	22.221479405556508
32	21.540286810428434	28.69753337326716	28.19051642991614	21.57166338638827
33	21.183823948884704	27.980596440184836	28.795585173141653	22.039994437788806
34	22.96230532260825	27.2561719151121	27.851702892349824	21.92981986992983
35	21.559540618403787	27.73181228250328	28.942395458953733	21.7662516401392
36	22.22183595755605	27.18298961720577	27.676011894574703	22.919162530663474
37	22.361871755376804	27.068447287352388	28.517474613497633	22.052206343773175
38	22.291809287466485	26.50286667807633	28.499201323521024	22.706122710936164
39	22.941536168634833	26.694245963831364	28.05449184209025	22.309726025443553
40	21.756713874151405	26.462665440127786	29.79473301386274	21.985887671858066
41	22.013253037823034	26.457673712134177	27.871848080324035	23.65722516971875
42	21.960215927890925	26.52381410804952	28.937582006959893	22.578387957099665
43	22.43264732728621	26.47505562211193	28.94096925095556	22.1513277996463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	38.5
2	45.0
3	182.0
4	319.0
5	319.0
6	379.0
7	439.0
8	463.0
9	487.0
10	761.5
11	1036.0
12	1036.0
13	1549.5
14	2063.0
15	4945.0
16	7827.0
17	8822.5
18	9818.0
19	9818.0
20	10274.5
21	10731.0
22	13363.5
23	15996.0
24	20546.5
25	25097.0
26	25097.0
27	30538.5
28	35980.0
29	41358.5
30	46737.0
31	51383.5
32	56030.0
33	56030.0
34	60535.5
35	65041.0
36	68320.0
37	71599.0
38	73532.5
39	75466.0
40	75466.0
41	75997.0
42	76528.0
43	76249.0
44	75970.0
45	76235.0
46	76500.0
47	76500.0
48	77467.0
49	78434.0
50	81540.0
51	84646.0
52	75467.5
53	66289.0
54	66289.0
55	67900.0
56	69511.0
57	60083.5
58	50656.0
59	43903.5
60	37151.0
61	37151.0
62	33200.5
63	29250.0
64	25120.0
65	20990.0
66	17196.0
67	13402.0
68	13402.0
69	11059.5
70	8717.0
71	7205.5
72	5694.0
73	3778.0
74	1862.0
75	1862.0
76	1315.0
77	768.0
78	593.0
79	418.0
80	300.5
81	183.0
82	183.0
83	137.5
84	92.0
85	62.0
86	32.0
87	23.5
88	15.0
89	15.0
90	10.0
91	5.0
92	2.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1121856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.04452048521119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88653313781118	39.494782284424716
2	14.304617246405076	14.889538906272684
3	4.476720112047014	6.989662547339631
4	1.835978025104187	3.822103837517298
5	0.9155005314884908	2.382339308263724
6	0.5321025373318686	1.6615812842640774
7	0.3271179469109633	1.1917287682361497
8	0.24922293427681305	1.0376550486683231
9	0.18112963004724897	0.8484124267325496
>10	1.0877433182823346	10.860203729646468
>50	0.12376960203027339	4.495106296111144
>100	0.07389404148642864	6.963723041745569
>500	0.0032650848115923126	1.1946957434637848
>1k	0.0018903122593429177	2.438402811034334
>5k	5.15539707093523E-4	1.7300639662796418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	7900	0.7041901990986366	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	6268	0.5587169832848422	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	5176	0.4613782874094358	RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4805	0.4283080894517657	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4657	0.415115665468652	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3956	0.3526299275486337	No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	3543	0.3158159335957556	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	1832	0.16330081579097494	RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	1789	0.15946788179588112	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1781	0.1587547777967939	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1621	0.14449269781504936	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1155	0.10295438986821838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.913799988590336E-5	0.0
10	0.0	0.0	0.0	8.913799988590336E-5	0.0
11	0.0	0.0	0.0	8.913799988590336E-5	0.0
12	0.0	0.0	0.0	8.913799988590336E-5	0.0
13	0.0	0.0	0.0	1.7827599977180672E-4	0.0
14	0.0	0.0	0.0	1.7827599977180672E-4	0.0
15	0.0	0.0	0.0	1.7827599977180672E-4	0.0
16	0.0	0.0	0.0	1.7827599977180672E-4	0.0
17	0.0	0.0	0.0	2.6741399965771005E-4	0.0
18	0.0	0.0	0.0	2.6741399965771005E-4	0.0
19	0.0	0.0	0.0	2.6741399965771005E-4	0.0
20	0.0	0.0	0.0	3.5655199954361344E-4	0.0
21	0.0	0.0	0.0	4.456899994295168E-4	0.0
22	0.0	0.0	0.0	7.131039990872269E-4	0.0
23	0.0	0.0	0.0	0.001247931998402647	0.0
24	0.0	0.0	0.0	0.001782759997718067	0.0
25	0.0	0.0	0.0	0.0018718979976039705	0.0
26	0.0	0.0	0.0	0.001961035997489874	0.0
27	0.0	0.0	0.0	0.0029415539962348108	0.0
28	0.0	0.0	0.0	0.00980517998744937	0.0
29	0.0	0.0	0.0	0.027276227965086428	0.0
30	0.0	0.0	0.0	0.04822365793827372	0.0
31	0.0	0.0	0.0	0.10206300986935934	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGAG	25	1.2336067E-4	37.0	35
CGTTACG	25	0.0054962877	29.6	16
GGTATCA	780	0.0	29.410254	1
GATTACG	95	0.0	29.210527	1
CTTATAC	1170	0.0	28.46154	1
GTATCAA	2520	0.0	24.960316	1
TATACAC	1435	0.0	24.623693	3
TTATACA	1410	0.0	24.141844	2
CGTCTTC	345	0.0	24.130436	37
CCGTCTT	930	0.0	23.274193	37
CGGCATT	40	0.0019311585	23.125	27
TACGTTA	40	0.0019311585	23.125	19
GCCGTCT	940	0.0	22.829786	36
GCTTTAT	875	0.0	21.777143	1
TTACGGG	140	0.0	21.142857	3
ACGTCCT	45	0.0038257516	20.555555	35
GATAGCG	45	0.0038257516	20.555555	2
CGCTTAA	45	0.0038257516	20.555555	1
CGCCGTT	55	5.1429874E-4	20.181818	25
AGGACCG	65	6.90194E-5	19.923077	5
>>END_MODULE