FastQCFastQC Report
Fri 10 Feb 2017
ERR1631116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831998
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT186432.240750578727353No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG146571.7616628886127128No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT132001.586542275342008RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC78710.9460359279709807No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC48820.5867802566833094RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC39280.4721165195108642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23300.2800487501171878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22920.27548143144574866No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC22600.2716352683540105No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG18820.22620246683285297No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA13940.1675484796838454No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT12250.14723593085560302No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG10540.126682996834127No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT10250.12319741153223926No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT9400.11298104081980966No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA9240.11105795927394058No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9020.10841372214837056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8900.10697141098896873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22400.033.366071
CGTCTTC6800.031.83088337
TTATACA24250.030.668042
TATACAC24600.030.2317073
CGAGCCT405.9381877E-527.7529
GATTACG754.620233E-1027.1333331
GGTATCA4900.026.428571
CTACCGA358.8673295E-426.4285725
CCGTCTT22500.026.22888837
GCCGTCT22850.025.82713336
CTCAACG400.001930741123.1254
TGCCGTC26750.022.13084235
GTATCAA12150.021.7736631
TTACGGG957.1377144E-921.4210533
ATGCCGT28400.020.8450734
CCTACCG450.003824933720.55555524
TACCGAG450.003824933720.55555526
TTAACGG1403.6379788E-1219.82142835
TAACGGC1500.019.73333436
TATGCCG30250.019.50909233
Produced by FastQC (version 0.11.5)