##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831998
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48004442318371	31.0	31.0	34.0	28.0	34.0
2	31.48923434912103	31.0	31.0	34.0	28.0	34.0
3	30.96010206755305	31.0	31.0	34.0	27.0	34.0
4	35.1055170805699	37.0	35.0	37.0	32.0	37.0
5	35.29968100884858	37.0	35.0	37.0	33.0	37.0
6	35.48296630520746	37.0	35.0	37.0	33.0	37.0
7	35.49184853809745	37.0	35.0	37.0	33.0	37.0
8	35.52074884795396	37.0	35.0	37.0	33.0	37.0
9	37.29654878978074	39.0	37.0	39.0	34.0	39.0
10	37.126711843057315	39.0	37.0	39.0	33.0	39.0
11	37.19101007454345	39.0	37.0	39.0	33.0	39.0
12	37.120812790415364	39.0	37.0	39.0	33.0	39.0
13	37.204095442537124	39.0	37.0	39.0	33.0	39.0
14	38.3345957562398	40.0	38.0	41.0	34.0	41.0
15	38.350243630393344	40.0	38.0	41.0	34.0	41.0
16	38.347089776658116	40.0	38.0	41.0	34.0	41.0
17	38.30810771179738	40.0	38.0	41.0	33.0	41.0
18	38.339744807078866	40.0	38.0	41.0	34.0	41.0
19	38.34940348414299	40.0	38.0	41.0	34.0	41.0
20	38.348802521159904	40.0	38.0	41.0	34.0	41.0
21	38.30942622458227	40.0	38.0	41.0	34.0	41.0
22	38.22621328416655	40.0	38.0	41.0	34.0	41.0
23	38.23861115050757	40.0	38.0	41.0	34.0	41.0
24	38.17736100327164	40.0	38.0	41.0	33.0	41.0
25	38.14161692696376	40.0	37.0	41.0	33.0	41.0
26	38.016900281010294	40.0	37.0	41.0	33.0	41.0
27	37.94287245882803	40.0	37.0	41.0	33.0	41.0
28	37.95423306306025	40.0	37.0	41.0	33.0	41.0
29	37.86436866434775	40.0	37.0	41.0	33.0	41.0
30	37.793110079591536	40.0	37.0	41.0	33.0	41.0
31	37.792634116908935	40.0	37.0	41.0	33.0	41.0
32	37.688036509703146	40.0	37.0	41.0	33.0	41.0
33	37.64694386284582	40.0	37.0	41.0	33.0	41.0
34	37.5842369813389	40.0	37.0	41.0	32.0	41.0
35	37.55547849874639	40.0	37.0	41.0	32.0	41.0
36	37.541277743456114	40.0	37.0	41.0	32.0	41.0
37	37.4778136485905	40.0	37.0	41.0	32.0	41.0
38	37.460980675434314	39.0	37.0	41.0	32.0	41.0
39	37.37484585299484	39.0	36.0	41.0	31.0	41.0
40	37.2575919653653	39.0	36.0	41.0	31.0	41.0
41	37.20577092733396	39.0	36.0	41.0	31.0	41.0
42	37.16791506710353	39.0	36.0	41.0	31.0	41.0
43	36.49146512371424	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	9.0
11	11.0
12	11.0
13	14.0
14	5.0
15	9.0
16	9.0
17	17.0
18	13.0
19	36.0
20	59.0
21	152.0
22	298.0
23	567.0
24	1036.0
25	1802.0
26	2966.0
27	4426.0
28	6773.0
29	9775.0
30	13441.0
31	18320.0
32	24018.0
33	31360.0
34	41777.0
35	56023.0
36	77028.0
37	114600.0
38	198023.0
39	229406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.468599684133856	21.820725530590217	15.13669504013231	25.573979745143617
2	13.99943269094397	20.90738198889901	41.33339262834767	23.759792691809356
3	14.299553604696166	28.32398635573643	34.12904838713555	23.247411652431857
4	11.62442698179563	19.238748170067716	36.92100221394763	32.215822634189024
5	12.627554393159599	38.078817497157445	35.384820636588074	13.908807473094885
6	28.16160615770711	39.368604251452524	17.404849530888296	15.064940059952066
7	24.84044432799117	32.83601643273181	22.210029351032095	20.113509888244923
8	25.354868641511157	34.62880920386828	22.027096218981296	17.98922593563927
9	23.88611511085363	14.727679633845273	18.870478054033786	42.51572720126731
10	15.057848696751675	28.267495835326528	32.71486229534206	23.95979317257974
11	31.093343974384553	22.650775602825966	25.167247998192305	21.088632424597176
12	20.86218957257109	26.184197558167206	27.8807160594136	25.0728968098481
13	28.920141634955854	19.752932098394467	26.69970360505674	24.62722266159294
14	21.910269015069748	21.962432602001446	25.71664835732778	30.410650025601022
15	24.4540251298681	28.09910600746636	22.232745751792674	25.214123110872862
16	25.543210440409712	26.21208224058231	22.85786744679675	25.38683987221123
17	20.97324753184503	30.746347947951797	24.083711739691683	24.19669278051149
18	24.269529494061285	25.338522448371272	24.869530936372445	25.522417121195
19	23.782509092569935	28.586366794150948	24.604145683042507	23.02697843023661
20	22.796088452135702	27.107997855764076	24.794891333873398	25.30102235822682
21	26.43672220365914	25.869292955031142	24.55642922218563	23.137555619124083
22	22.14752920079135	31.667984778809565	24.63046786170159	21.554018158697495
23	26.06953382099476	24.9385214868305	24.83503566114342	24.156909031031322
24	25.381791783153325	28.37578936487828	24.193207195209602	22.04921165675879
25	22.52445318378169	28.380957646532806	24.972896569462907	24.121692600222598
26	25.47794586044678	29.033242868372277	24.972896569462907	20.51591470171803
27	22.034307774778306	31.074834314505562	24.650419832739985	22.240438077976147
28	23.988038432784695	25.161118175764845	26.880353077771822	23.97049031367864
29	23.08527183959577	28.907641604907703	25.46171985990351	22.54536669559302
30	23.569047040978464	27.10811804836069	26.877708840646253	22.44512607001459
31	26.715448835213547	25.390686035302966	25.01748802280775	22.87637710667574
32	22.72553539792163	27.40595530277717	26.293212243298665	23.57529705600254
33	23.817605330782047	27.003310104110827	27.22278178553314	21.95630277957399
34	24.3611162526833	27.675066526602226	25.637922206543767	22.325895014170708
35	22.3201257695331	24.853304935829172	29.785768715790184	23.040800578847545
36	23.974456669366994	27.571220123125293	23.960874905949293	24.493448301558416
37	22.33478926632035	26.50871756903262	27.40655626576026	23.749936898886777
38	22.978540813800034	24.38383265344388	27.86689391080267	24.770732621953417
39	24.4418856776098	25.223137555619125	26.084197317782014	24.25077944898906
40	21.752816713501716	23.87493719936827	31.908369972043204	22.463876115086816
41	22.64849194349025	25.29272906906026	25.926624823617367	26.132154163832123
42	23.120007500018026	24.623496691097817	29.250551083055488	23.005944725828666
43	23.532388299010336	23.2534212822627	29.757042685198765	23.457147733528206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	9.5
2	10.0
3	74.5
4	139.0
5	139.0
6	177.0
7	215.0
8	233.5
9	252.0
10	359.5
11	467.0
12	467.0
13	753.5
14	1040.0
15	2388.5
16	3737.0
17	4160.0
18	4583.0
19	4583.0
20	4598.5
21	4614.0
22	5381.0
23	6148.0
24	8246.5
25	10345.0
26	10345.0
27	12975.0
28	15605.0
29	18894.5
30	22184.0
31	26020.5
32	29857.0
33	29857.0
34	34846.0
35	39835.0
36	43503.0
37	47171.0
38	50886.0
39	54601.0
40	54601.0
41	56836.0
42	59071.0
43	59946.0
44	60821.0
45	60509.0
46	60197.0
47	60197.0
48	59572.5
49	58948.0
50	73453.5
51	87959.0
52	69025.5
53	50092.0
54	50092.0
55	60900.0
56	71708.0
57	60556.0
58	49404.0
59	37882.5
60	26361.0
61	26361.0
62	24292.0
63	22223.0
64	17608.5
65	12994.0
66	10543.5
67	8093.0
68	8093.0
69	6903.5
70	5714.0
71	4961.0
72	4208.0
73	5227.5
74	6247.0
75	6247.0
76	5515.0
77	4783.0
78	3331.5
79	1880.0
80	1084.5
81	289.0
82	289.0
83	199.5
84	110.0
85	79.5
86	49.0
87	37.5
88	26.0
89	26.0
90	15.5
91	5.0
92	3.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831998.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.787114624921145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61217756249548	34.422004298436434
2	12.247392445208893	10.725559537095101
3	3.5582539535436664	4.674170211851861
4	1.5389741138737831	2.695489437159112
5	0.8177813619386071	1.7904143116664957
6	0.5100370196827239	1.3399829666280356
7	0.3541098981443091	1.085381548990481
8	0.26428891037633584	0.9257959050195305
9	0.21301020805245188	0.8394392156643912
>10	1.5433639276156335	13.755033763206029
>50	0.2005052603415988	6.065494277236427
>100	0.13045241184581954	10.66985704194998
>500	0.0057917561284470415	1.755666266857446
>1k	0.0027579791087843056	2.689654635812975
>5k	2.7579791087843056E-4	0.9505330297451476
>10k+	8.273937326352916E-4	5.615523552680647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	18643	2.240750578727353	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	14657	1.7616628886127128	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	13200	1.586542275342008	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	7871	0.9460359279709807	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	4882	0.5867802566833094	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	3928	0.4721165195108642	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2330	0.2800487501171878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2292	0.27548143144574866	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	2260	0.2716352683540105	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1882	0.22620246683285297	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1394	0.1675484796838454	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1225	0.14723593085560302	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1054	0.126682996834127	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1025	0.12319741153223926	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	940	0.11298104081980966	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	924	0.11105795927394058	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	902	0.10841372214837056	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	890	0.10697141098896873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.2019259661681879E-4
7	0.0	0.0	0.0	0.0	1.2019259661681879E-4
8	0.0	0.0	0.0	0.0	1.2019259661681879E-4
9	0.0	0.0	0.0	3.6057778985045636E-4	1.2019259661681879E-4
10	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
11	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
12	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
13	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
14	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
15	0.0	0.0	0.0	4.8077038646727517E-4	1.2019259661681879E-4
16	0.0	0.0	0.0	8.413481763177316E-4	1.2019259661681879E-4
17	0.0	0.0	0.0	8.413481763177316E-4	1.2019259661681879E-4
18	0.0	0.0	0.0	8.413481763177316E-4	2.4038519323363758E-4
19	0.0	0.0	0.0	8.413481763177316E-4	2.4038519323363758E-4
20	0.0	0.0	0.0	9.615407729345503E-4	4.8077038646727517E-4
21	0.0	0.0	0.0	0.0015625037560186442	6.00962983084094E-4
22	0.0	0.0	0.0	0.0015625037560186442	6.00962983084094E-4
23	0.0	0.0	0.0	0.001802888949252282	6.00962983084094E-4
24	0.0	0.0	0.0	0.002283659335719557	6.00962983084094E-4
25	0.0	0.0	0.0	0.002403851932336376	6.00962983084094E-4
26	0.0	0.0	0.0	0.002884622318803651	6.00962983084094E-4
27	0.0	0.0	0.0	0.0037259704951213826	6.00962983084094E-4
28	1.2019259661681879E-4	0.0	0.0	0.009495215132728685	6.00962983084094E-4
29	1.2019259661681879E-4	0.0	0.0	0.021153897004560107	6.00962983084094E-4
30	1.2019259661681879E-4	0.0	0.0	0.03725970495121383	6.00962983084094E-4
31	1.2019259661681879E-4	0.0	0.0	0.07680306923814721	6.00962983084094E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2240	0.0	33.36607	1
CGTCTTC	680	0.0	31.830883	37
TTATACA	2425	0.0	30.66804	2
TATACAC	2460	0.0	30.231707	3
CGAGCCT	40	5.9381877E-5	27.75	29
GATTACG	75	4.620233E-10	27.133333	1
GGTATCA	490	0.0	26.42857	1
CTACCGA	35	8.8673295E-4	26.42857	25
CCGTCTT	2250	0.0	26.228888	37
GCCGTCT	2285	0.0	25.827133	36
CTCAACG	40	0.0019307411	23.125	4
TGCCGTC	2675	0.0	22.130842	35
GTATCAA	1215	0.0	21.773663	1
TTACGGG	95	7.1377144E-9	21.421053	3
ATGCCGT	2840	0.0	20.84507	34
CCTACCG	45	0.0038249337	20.555555	24
TACCGAG	45	0.0038249337	20.555555	26
TTAACGG	140	3.6379788E-12	19.821428	35
TAACGGC	150	0.0	19.733334	36
TATGCCG	3025	0.0	19.509092	33
>>END_MODULE