FastQCFastQC Report
Fri 10 Feb 2017
ERR1631115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086205
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT71700.6600963906444917No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG58020.5341533136010238No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT40800.3756197034629743TruSeq Adapter, Index 5 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39900.36733397471011453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38490.35435299966396766No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC33620.30951800074571556No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21520.19812098084615706No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC15850.14592088970314077No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC15090.13892405208961475Illumina Paired End PCR Primer 2 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13250.1219843399726571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13070.12032719422208515No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11680.10753034648155735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG1000.029.5999981
CTTATAC10900.027.1559641
GTACCGA358.8690786E-426.4285726
TTATACA11750.026.1361712
TATACAC11850.026.071733
TTACGGG1150.025.739133
GGTATCA6900.025.20291
GTATCAA19800.023.8257581
CGTTCCA400.001931119523.12532
GCCGTCT7150.023.02797136
CCGTCTT7350.022.4013637
CCGTTTA1201.0913936E-1121.58333227
CGTCTTC3200.020.812537
CATATCG450.003825674320.55555737
TGTTACG909.477117E-820.55555716
TTTACCG1351.8189894E-1220.55555530
GTTACGG1355.638867E-1119.18518617
CGCCGTT1355.638867E-1119.18518625
TTACCGG1355.638867E-1119.18518631
AGGACCG1457.2759576E-1219.1379325
Produced by FastQC (version 0.11.5)