ERR1631114.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208562
Sequences flagged as poor quality	0
Sequence length	43
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	12096	5.799714233657138	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	11273	5.405107354168065	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	9585	4.595755698545276	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	6112	2.9305434355251676	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	3811	1.8272743836365208	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2998	1.4374622414437912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.8453121853453649	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1619	0.776267968278018	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1454	0.6971548028883497	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1057	0.5068037322235115	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	878	0.42097793461896227	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	712	0.34138529549965957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.312137397991964	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	590	0.2828895004842685	No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT	343	0.1644594892645832	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	342	0.16398001553494884	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	325	0.15582896213116484	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	313	0.1500752773755526	No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	274	0.1313758019198128	TruSeq Adapter, Index 10 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	272	0.1304168544605441	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	247	0.11843001121968529	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	244	0.11699159003078222	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	243	0.11651211630114786	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	238	0.11411474765297609	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	229	0.10979948408626691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.1016484306824829	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCTTC	30	8.279378E-6	37.0	37
TGTGCTA	40	3.8076905E-8	37.0	26
TCTGTGC	35	5.601887E-7	37.0	24
CTCTCTG	35	5.601887E-7	37.0	21
GATTACG	20	0.0018384841	37.0	1
GAGGCAC	20	0.0018384841	37.0	7
CTTATAC	1305	0.0	35.582375	1
CGTCTTC	475	0.0	35.052635	37
TTATACA	1425	0.0	32.585964	2
GGTATCA	125	0.0	32.559998	1
GTGCTAG	40	1.5917667E-6	32.375	27
CTCTGTG	40	1.5917667E-6	32.375	23
TGCTAGT	40	1.5917667E-6	32.375	28
CTGTGCT	40	1.5917667E-6	32.375	25
GACCGTT	35	2.3790128E-5	31.714287	7
ATTACCG	35	2.3790128E-5	31.714287	5
AGGACCG	35	2.3790128E-5	31.714287	5
TACGGCC	35	2.3790128E-5	31.714287	19
GCCGCCG	35	2.3790128E-5	31.714287	23
CGCCGTT	35	2.3790128E-5	31.714287	25

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content