##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208562
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26074260891246	31.0	31.0	34.0	28.0	34.0
2	31.31593003519337	31.0	31.0	34.0	28.0	34.0
3	30.788580853655027	31.0	31.0	34.0	27.0	34.0
4	34.9244685033707	37.0	35.0	37.0	32.0	37.0
5	35.09975450945043	35.0	35.0	37.0	32.0	37.0
6	35.30840229763811	37.0	35.0	37.0	33.0	37.0
7	35.28057843710743	37.0	35.0	37.0	32.0	37.0
8	35.293644096239966	37.0	35.0	37.0	32.0	37.0
9	37.093996029957516	39.0	37.0	39.0	33.0	39.0
10	36.921956061027416	39.0	37.0	39.0	33.0	39.0
11	36.957072716985834	39.0	37.0	39.0	33.0	39.0
12	36.85842099711357	39.0	37.0	39.0	33.0	39.0
13	36.95854470133581	39.0	37.0	39.0	33.0	39.0
14	37.853257065045405	39.0	37.0	41.0	33.0	41.0
15	37.86103412894008	39.0	37.0	41.0	33.0	41.0
16	37.828142231087156	39.0	37.0	41.0	33.0	41.0
17	37.82440233599601	39.0	37.0	41.0	33.0	41.0
18	37.915468781465464	40.0	37.0	41.0	33.0	41.0
19	37.87318399324901	40.0	37.0	41.0	33.0	41.0
20	37.921246439907556	40.0	37.0	41.0	33.0	41.0
21	37.80037590740403	40.0	37.0	41.0	32.0	41.0
22	37.68893182842512	39.0	37.0	41.0	32.0	41.0
23	37.723055973763195	39.0	37.0	41.0	32.0	41.0
24	37.63486637067155	39.0	37.0	41.0	32.0	41.0
25	37.629592159645576	39.0	37.0	41.0	32.0	41.0
26	37.4188346870475	39.0	36.0	41.0	32.0	41.0
27	37.19754797134665	39.0	36.0	41.0	31.0	41.0
28	37.189075670544014	39.0	36.0	41.0	31.0	41.0
29	37.165615020953005	39.0	36.0	40.0	31.0	41.0
30	37.0382955667859	39.0	36.0	40.0	31.0	41.0
31	36.88603388920321	39.0	36.0	40.0	31.0	41.0
32	36.68859140207708	39.0	35.0	40.0	30.0	41.0
33	36.52594911824781	39.0	35.0	40.0	30.0	41.0
34	36.43137771981473	39.0	35.0	40.0	30.0	41.0
35	36.53954699322024	39.0	35.0	40.0	30.0	41.0
36	36.57410266491499	39.0	35.0	40.0	30.0	41.0
37	36.36432331872537	39.0	35.0	40.0	30.0	41.0
38	36.317881493272985	39.0	35.0	40.0	30.0	41.0
39	36.16332793126264	39.0	35.0	40.0	29.0	41.0
40	35.80082181797259	38.0	35.0	40.0	27.0	41.0
41	35.799953970521955	38.0	35.0	40.0	28.0	41.0
42	35.64704979814156	38.0	34.0	40.0	28.0	41.0
43	34.56830582752371	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	4.0
10	11.0
11	9.0
12	9.0
13	7.0
14	8.0
15	2.0
16	5.0
17	8.0
18	17.0
19	24.0
20	46.0
21	96.0
22	180.0
23	338.0
24	486.0
25	904.0
26	1369.0
27	1985.0
28	2854.0
29	3750.0
30	4812.0
31	6063.0
32	7742.0
33	9669.0
34	12433.0
35	15771.0
36	21649.0
37	31465.0
38	47284.0
39	39558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.54918920992319	24.71639128892128	17.72518483712277	27.009234664032753
2	11.773956904901182	19.89432398998859	43.51655622788427	24.81516287722596
3	12.250553792157728	31.273194541671064	36.46349766496294	20.012754001208275
4	8.969515060269847	21.040266203814692	30.67624974827629	39.31396898763917
5	11.595592677477201	38.39817416403755	33.576586338834495	16.42964681965075
6	25.369913982412907	38.6848994543589	15.984695198550073	19.96049136467813
7	21.208082009186715	34.40080168007595	18.3173348932212	26.073781417516134
8	25.824934551835906	31.131270317699293	24.36062178153259	18.68317334893221
9	19.888570305232978	12.494605920541614	15.247744076102071	52.36907969812334
10	14.691075075996588	27.25904047717226	30.25527181365733	27.794612633173827
11	28.082776344684074	20.464418254523835	24.23547913809802	27.21732626269407
12	17.602919036066016	28.64951429311188	21.53891888263442	32.20864778818768
13	33.21458367296056	16.435879978135997	25.00647289535006	25.343063453553377
14	22.536703714003508	24.885645515482206	21.40226886968863	31.175381900825656
15	26.834226752716216	27.040879930188627	18.080474870781828	28.044418446313323
16	29.334202779029738	22.903980590903426	18.788178095722134	28.973638534344705
17	17.978826440099347	35.52037283877216	19.315599198319923	27.185201522808565
18	25.852264554425062	19.374094993335316	20.753061439763716	34.020579012475906
19	24.34000441115831	26.037820887793554	21.64680047180215	27.97537422924598
20	24.451721790163116	23.65915171507753	21.17116253200487	30.71796396275448
21	28.605882183715153	25.116751853165965	21.18746463881244	25.08990132430644
22	25.92802140370729	34.06804691170971	21.352403601806657	18.651528082776345
23	28.83555010021001	20.861901976390715	21.741256796540117	28.56129112685916
24	25.1004497463584	26.389275131615538	22.922680066359163	25.587595055666903
25	24.684266549035776	27.123349411685734	26.381124078211755	21.811259961066735
26	29.092548019294025	25.25388133984139	21.443503610437183	24.210067030427403
27	26.44345566306422	30.05676968958871	24.992088683461034	18.50768596388604
28	21.17451884811231	25.37183187733144	27.523230502200786	25.930418772355463
29	24.233081769449853	20.746828281278468	23.994303852091946	31.025786097179736
30	30.109991273578117	20.44428035787919	30.575560265053074	18.87016810348961
31	24.74084444913263	25.448547674072934	21.286715700846752	28.52389217594768
32	28.08661213452115	22.23703263298204	31.61122352106328	18.06513171143353
33	29.083438018430968	20.68833248626308	30.54870973619355	19.6795197591124
34	18.5992654462462	28.796712728109625	25.32004871453093	27.28397311111324
35	19.682396601490204	19.737536080398154	43.19722672394779	17.382840594163845
36	29.342833306163158	21.68324047525436	21.44446255789645	27.529463660686034
37	20.42941667226053	25.651844535437903	29.689013338959157	24.22972545334241
38	20.173857174365416	20.169062437069073	30.80570765527757	28.851372733287945
39	27.354455749369492	21.959896817253384	25.39676451127243	25.2888829221047
40	17.654702198866524	19.37793078317239	41.612566047506256	21.35480097045483
41	22.648421093008313	23.835598047582973	23.594902235306527	29.921078624102186
42	22.784112158494835	19.933640835818604	35.66373548393283	21.61851152175372
43	23.762238566948916	18.96174758584977	33.53535159808594	23.74066224911537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42.0
1	33.0
2	24.0
3	52.0
4	80.0
5	80.0
6	109.5
7	139.0
8	144.5
9	150.0
10	241.5
11	333.0
12	333.0
13	480.0
14	627.0
15	1577.0
16	2527.0
17	2503.5
18	2480.0
19	2480.0
20	2011.0
21	1542.0
22	1220.5
23	899.0
24	1079.0
25	1259.0
26	1259.0
27	1526.0
28	1793.0
29	2165.0
30	2537.0
31	3068.0
32	3599.0
33	3599.0
34	4608.0
35	5617.0
36	6582.5
37	7548.0
38	8918.5
39	10289.0
40	10289.0
41	10741.0
42	11193.0
43	11823.0
44	12453.0
45	13416.5
46	14380.0
47	14380.0
48	14643.0
49	14906.0
50	14154.0
51	13402.0
52	14213.0
53	15024.0
54	15024.0
55	25724.0
56	36424.0
57	22811.5
58	9199.0
59	16082.5
60	22966.0
61	22966.0
62	17861.0
63	12756.0
64	7513.5
65	2271.0
66	1650.5
67	1030.0
68	1030.0
69	794.5
70	559.0
71	417.5
72	276.0
73	189.0
74	102.0
75	102.0
76	79.5
77	57.0
78	42.5
79	28.0
80	25.0
81	22.0
82	22.0
83	15.0
84	8.0
85	7.5
86	7.0
87	6.0
88	5.0
89	5.0
90	6.5
91	8.0
92	4.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	208562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.17926563803569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19047619047619	18.898936527267672
2	10.456043956043956	5.474631044965046
3	4.423076923076923	3.47378717120089
4	2.600732600732601	2.7234107843231268
5	1.7216117216117217	2.253526529281461
6	1.3498168498168497	2.12023283244311
7	0.9743589743589745	1.7855601691583316
8	0.8168498168498168	1.7107622673353726
9	0.5915750915750916	1.3938301320470652
>10	4.336996336996337	21.92777207736788
>50	0.3827838827838828	6.732770111525589
>100	0.13003663003663005	5.325994188778397
>500	0.007326007326007326	1.3573901285948544
>1k	0.010989010989010988	6.090275313815556
>5k	0.003663003663003663	7.5262991340704435
>10k+	0.003663003663003663	11.204821587825203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	12096	5.799714233657138	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	11273	5.405107354168065	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	9585	4.595755698545276	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	6112	2.9305434355251676	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	3811	1.8272743836365208	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2998	1.4374622414437912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1763	0.8453121853453649	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1619	0.776267968278018	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1454	0.6971548028883497	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1057	0.5068037322235115	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	878	0.42097793461896227	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	712	0.34138529549965957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	651	0.312137397991964	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	590	0.2828895004842685	No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT	343	0.1644594892645832	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	342	0.16398001553494884	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	325	0.15582896213116484	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	313	0.1500752773755526	No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	274	0.1313758019198128	TruSeq Adapter, Index 10 (95% over 23bp)
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	272	0.1304168544605441	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	247	0.11843001121968529	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	244	0.11699159003078222	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	243	0.11651211630114786	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	238	0.11411474765297609	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	229	0.10979948408626691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.1016484306824829	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	4.794737296343533E-4	0.0
18	0.0	0.0	0.0	4.794737296343533E-4	0.0
19	0.0	0.0	0.0	4.794737296343533E-4	0.0
20	0.0	0.0	0.0	9.589474592687066E-4	0.0
21	0.0	0.0	0.0	0.0019178949185374133	0.0
22	0.0	0.0	0.0	0.00287684237780612	0.0
23	0.0	0.0	0.0	0.0033563161074404734	0.0
24	0.0	0.0	0.0	0.0052742110259778865	0.0
25	0.0	0.0	0.0	0.0052742110259778865	0.0
26	0.0	0.0	0.0	0.00575368475561224	0.0
27	0.0	0.0	0.0	0.00575368475561224	0.0
28	0.0	0.0	0.0	0.01294579070012754	0.0
29	0.0	0.0	0.0	0.03548105599294215	0.0
30	0.0	0.0	0.0	0.06568790095990641	0.0
31	0.0	0.0	0.0	0.10404579933065468	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCTTC	30	8.279378E-6	37.0	37
TGTGCTA	40	3.8076905E-8	37.0	26
TCTGTGC	35	5.601887E-7	37.0	24
CTCTCTG	35	5.601887E-7	37.0	21
GATTACG	20	0.0018384841	37.0	1
GAGGCAC	20	0.0018384841	37.0	7
CTTATAC	1305	0.0	35.582375	1
CGTCTTC	475	0.0	35.052635	37
TTATACA	1425	0.0	32.585964	2
GGTATCA	125	0.0	32.559998	1
GTGCTAG	40	1.5917667E-6	32.375	27
CTCTGTG	40	1.5917667E-6	32.375	23
TGCTAGT	40	1.5917667E-6	32.375	28
CTGTGCT	40	1.5917667E-6	32.375	25
GACCGTT	35	2.3790128E-5	31.714287	7
ATTACCG	35	2.3790128E-5	31.714287	5
AGGACCG	35	2.3790128E-5	31.714287	5
TACGGCC	35	2.3790128E-5	31.714287	19
GCCGCCG	35	2.3790128E-5	31.714287	23
CGCCGTT	35	2.3790128E-5	31.714287	25
>>END_MODULE