FastQCFastQC Report
Fri 10 Feb 2017
ERR1631113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868948
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT78470.9030459820380506No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG68210.7849721732485718No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT47160.5427252263656743TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC38430.44225891537813544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31690.3646938597016162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.3358083567716365No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC18520.21313128058295777No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC15330.17642022307433816RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG10150.11680791025469879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9860.11347054139027883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG702.0736479E-1029.0714281
CTTATAC11350.028.6872251
TTATACA11750.028.3404252
TATACAC12350.027.4129563
CGGATCG556.258797E-726.9090937
GGTATCA4550.026.8351671
TTTACCG358.867648E-426.4285730
TTACGGG706.5883796E-926.428573
CGCCGTT358.867648E-426.4285725
CGTCTTC2450.025.67346837
GCCGTCT7600.025.3157936
GTATCAA15350.025.1889251
CCGTCTT7750.024.82580637
CGAACTA851.9754225E-923.94117529
TTACCGG400.001930809723.12500231
AGGACCG450.00382506820.5555555
AGCTGCG450.00382506820.55555528
GACCTAG1351.8189894E-1220.55555511
GCGAACT1001.288754E-820.3528
TACGGTG555.1417074E-420.18181828
Produced by FastQC (version 0.11.5)