FastQCFastQC Report
Fri 10 Feb 2017
ERR1631112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600364
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT150892.5133085927870424No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT142942.3808889273840537RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG137212.2854468289237864No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC78091.3007109020527545No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC54500.9077826118821248RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC40510.6747573138962363RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG19550.3256357809595512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16920.2818290237256065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16570.27599922713553776No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA13380.22286479535748313No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT11630.19371581240713967No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11230.18705318773277543No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11060.18422157224617064No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10390.1730616759166106No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC9430.15707137669813645No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8620.14357956173254893No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8530.14208047118081696No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8470.14108107747966234No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8050.1340853215715799No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT7620.12692300004663837No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7220.12026037537227416No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6670.11109926644502334No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6670.11109926644502334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6220.10360381368636361No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6180.10293755121892718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18550.034.2075461
TCGCCGA352.3864211E-531.7142871
GATTACG352.3864211E-531.7142871
CGTCTTC7950.031.18239237
TTATACA20600.030.9830072
CTGTCCG303.5974485E-430.83333211
TATACAC20850.030.7889693
ACGTTAT250.00549427829.61
GCGTCAC250.00549427829.626
TGTCCGA250.00549427829.612
CGCCGAG405.9354745E-527.7500022
GGTTGTG358.864438E-426.42857217
GGTATCA2200.026.0681821
GTATCAA7800.025.8525661
GCCGAGC551.9006966E-523.5454543
GACTATT551.9006966E-523.5454547
CCGTCTT22050.023.49206437
GCCGTCT22200.023.33333436
CGAGGGT400.001930118223.12500216
TTACGGG400.001930118223.1250023
Produced by FastQC (version 0.11.5)