FastQCFastQC Report
Fri 10 Feb 2017
ERR1631111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755034
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG84921.1247175623879189No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT69340.9183692390011575No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT61850.8191684083100894RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC49500.655599615381559No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA39060.5173276965010848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33200.43971529758924766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32270.4273979714820789No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC23320.30886026324642335No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC20710.27429228352630475RNA PCR Primer, Index 47 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12170.1611847943271429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11920.15787368515854913No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG11500.1523110217553117No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA10960.1451590259511492No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC9280.12290837233819933No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7880.10436616099407443No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7810.1034390504268682No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC7710.1021146067594307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10650.030.572771
CGTTATT250.005495164529.5999982
ACAATCG250.005495164529.59999837
GATTACG502.718698E-729.5999981
GGTATCA5500.029.2636381
TATACAC11200.028.9062483
TTATACA11500.027.8304332
GCAACGC556.256978E-726.909093
CACGCAA358.8665646E-426.4285715
CGTCTTC3250.025.61538537
CCGTCTT9150.024.66666637
GCCGTCT9300.024.26881636
GGGCCGC400.001930576623.12500233
GTATCAA15550.023.0803871
TATTGAG7250.022.9655175
AGCAACG652.6808211E-622.769232
TTATTGA7550.022.2980124
GTCTAAT502.700913E-422.1999991
TTACGGG1005.329639E-1022.1999993
GCTTTAT7500.021.9533351
Produced by FastQC (version 0.11.5)