##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	755034
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.76343979211532	31.0	30.0	33.0	27.0	34.0
2	30.729645287496986	31.0	30.0	34.0	27.0	34.0
3	30.138964602918545	31.0	30.0	34.0	25.0	34.0
4	34.42161412598638	35.0	35.0	37.0	30.0	37.0
5	34.44869370121081	35.0	35.0	37.0	30.0	37.0
6	34.69321116664945	35.0	35.0	37.0	32.0	37.0
7	34.73066908245192	35.0	35.0	37.0	32.0	37.0
8	34.76729922096224	35.0	35.0	37.0	32.0	37.0
9	36.43038326750848	38.0	35.0	39.0	32.0	39.0
10	36.15924978212902	37.0	35.0	39.0	32.0	39.0
11	36.27734115284875	38.0	35.0	39.0	32.0	39.0
12	36.174545782044255	37.0	35.0	39.0	32.0	39.0
13	36.308657623365306	38.0	35.0	39.0	32.0	39.0
14	37.23093662007274	39.0	36.0	40.0	32.0	41.0
15	37.24395987465465	39.0	36.0	40.0	32.0	41.0
16	37.236064865953054	39.0	36.0	40.0	32.0	41.0
17	37.198923492187106	39.0	36.0	40.0	32.0	41.0
18	37.253934259914125	39.0	36.0	40.0	32.0	41.0
19	37.26331132107958	39.0	36.0	40.0	32.0	41.0
20	37.28741752027061	39.0	36.0	40.0	32.0	41.0
21	37.217282930305124	39.0	36.0	40.0	32.0	41.0
22	37.09449640678433	39.0	36.0	40.0	31.0	41.0
23	37.118511219362304	39.0	36.0	40.0	31.0	41.0
24	37.043930472005236	39.0	36.0	40.0	31.0	41.0
25	37.027364330612926	39.0	36.0	40.0	31.0	41.0
26	36.86171748557019	39.0	36.0	40.0	31.0	41.0
27	36.754977921524066	39.0	36.0	40.0	31.0	41.0
28	36.716227348702176	38.0	36.0	40.0	30.0	41.0
29	36.614972305882915	38.0	35.0	40.0	30.0	41.0
30	36.539207505887155	38.0	35.0	40.0	30.0	41.0
31	36.50550173899453	38.0	35.0	40.0	30.0	41.0
32	36.37799092491199	38.0	35.0	40.0	30.0	41.0
33	36.36981645859657	38.0	35.0	40.0	30.0	41.0
34	36.26426359607647	38.0	35.0	40.0	30.0	41.0
35	36.201928654868524	38.0	35.0	40.0	30.0	41.0
36	36.19752620411796	38.0	35.0	40.0	30.0	41.0
37	36.09488844210989	38.0	35.0	40.0	30.0	41.0
38	36.0478243363875	38.0	35.0	40.0	29.0	41.0
39	35.95844293104681	38.0	35.0	40.0	29.0	41.0
40	35.84635261458424	38.0	34.0	40.0	29.0	41.0
41	35.803479048625626	38.0	34.0	40.0	29.0	41.0
42	35.73131011318696	38.0	34.0	40.0	28.0	41.0
43	34.96135670711518	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	2.0
12	10.0
13	4.0
14	10.0
15	9.0
16	8.0
17	17.0
18	36.0
19	88.0
20	156.0
21	347.0
22	596.0
23	1136.0
24	1908.0
25	2974.0
26	5003.0
27	7338.0
28	10453.0
29	14738.0
30	19456.0
31	25738.0
32	32751.0
33	42330.0
34	55946.0
35	73506.0
36	100435.0
37	136798.0
38	159177.0
39	64052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32564626228753	22.656728041386216	15.682075244293634	22.335550452032624
2	15.242227502337643	23.36543784783202	38.56554804154515	22.82678660828519
3	16.60428536993036	28.375543353014564	33.621532275367734	21.398639001687343
4	12.405798944153508	18.90113557800046	39.091749510618065	29.601315967227965
5	12.769623619598589	38.461844102384795	35.300661956944985	13.467870321071635
6	27.45094922877645	41.06927635046899	17.34425204692769	14.135522373826875
7	24.731071713326816	33.38021863916062	23.090880675572226	18.797828971940337
8	25.534744130727887	33.99992583115463	22.39925619243637	18.06607384568112
9	25.13701369739641	14.905553922075033	19.91989764699338	40.03753473353518
10	14.912043696045476	29.09219982146499	33.40326395897403	22.5924925235155
11	32.68223682642106	23.62449902918279	24.688557071602073	19.004707072794073
12	21.741007689719932	26.663435024118122	29.14742912239714	22.44812816376481
13	29.440793394734545	21.202356450173106	26.218554396225862	23.13829575886649
14	21.606179324374796	22.178471433074538	28.164294588058286	28.05105465449238
15	24.115072963601637	29.426489403126215	23.382258282408475	23.076179350863672
16	23.181207733691465	27.808151685884347	24.721933052021498	24.288707528402696
17	22.099269701761777	29.353115223950184	25.444814405708883	23.102800668579164
18	23.41854803889626	26.223454837795384	27.230694246881598	23.127302876426757
19	24.965895575563486	27.54909050453357	26.95123663305229	20.533777286850658
20	24.36552526111407	25.911945687214082	26.93309175480839	22.78943729686345
21	25.11754437548508	26.0918051372521	26.63482704090147	22.155823446361357
22	21.924178248926538	29.12848957795278	27.37558838409926	21.571743789021422
23	24.33956616523229	25.802414195917006	26.89998066312246	22.958038975728247
24	22.274096265863523	27.832521449365196	26.972295287364545	22.92108699740674
25	22.95141675739106	28.282699851927195	26.942362860480458	21.82352053020129
26	24.904176500660895	27.500086088838383	26.251135710444828	21.34460170005589
27	22.347338000672817	27.895035190468242	27.88338008619479	21.874246722664147
28	23.907532640914184	26.207826402519625	27.51187363747858	22.372767319087615
29	22.212642079694426	27.967879592177308	26.508210226294445	23.311268101833825
30	23.969781493283744	26.62913193313149	28.111846618827762	21.289239954757004
31	24.247782219078875	25.77645510003523	26.382520522254627	23.593242158631266
32	22.088011930588554	28.030525777647096	27.394527928543617	22.48693436322073
33	23.113396217918663	26.696943448904285	28.47673084920679	21.71292948397026
34	22.811423061742914	27.13970496692864	26.87190245737278	23.176969513955665
35	21.89702715374407	27.104872098475035	29.25020595099029	21.74789479679061
36	23.80608025598847	26.4529809253623	26.73561720399346	23.005321614655763
37	23.091278008672457	26.166106426995338	28.532092594505677	22.210522969826524
38	22.897511900126354	25.39024732661046	28.14005726894418	23.57218350431901
39	24.18526847797583	26.027437175014633	27.22301247361046	22.56428187339908
40	21.967222668118257	25.386009106874656	30.413199935367146	22.233568289639937
41	22.59646585451781	25.94598388946723	26.98381794727125	24.473732308743713
42	22.2301247361046	25.604939645102075	29.216829970570863	22.948105648222462
43	23.24570814029567	25.708113806795456	28.541231255810995	22.50494679709788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	31.5
2	35.0
3	102.0
4	169.0
5	169.0
6	250.0
7	331.0
8	318.5
9	306.0
10	473.0
11	640.0
12	640.0
13	1012.5
14	1385.0
15	3367.0
16	5349.0
17	5985.0
18	6621.0
19	6621.0
20	6896.0
21	7171.0
22	8726.5
23	10282.0
24	13045.5
25	15809.0
26	15809.0
27	18551.0
28	21293.0
29	24364.0
30	27435.0
31	30377.0
32	33319.0
33	33319.0
34	36092.5
35	38866.0
36	41361.0
37	43856.0
38	44358.0
39	44860.0
40	44860.0
41	46045.5
42	47231.0
43	47881.0
44	48531.0
45	49767.5
46	51004.0
47	51004.0
48	52484.0
49	53964.0
50	52371.0
51	50778.0
52	56805.0
53	62832.0
54	62832.0
55	53390.0
56	43948.0
57	44351.5
58	44755.0
59	38777.0
60	32799.0
61	32799.0
62	27294.5
63	21790.0
64	18865.5
65	15941.0
66	13178.5
67	10416.0
68	10416.0
69	8429.5
70	6443.0
71	5426.5
72	4410.0
73	2871.0
74	1332.0
75	1332.0
76	929.0
77	526.0
78	407.5
79	289.0
80	229.0
81	169.0
82	169.0
83	113.0
84	57.0
85	50.0
86	43.0
87	27.5
88	12.0
89	12.0
90	10.0
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	755034.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.63342209530168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.71613306560013	41.676501684766684
2	11.448120614928524	11.822112878170584
3	3.2542861602557127	5.040897927941454
4	1.3531251480053568	2.7946592765891265
5	0.6924493460303006	1.7876764681599062
6	0.4290255164446511	1.329123334814488
7	0.28557558176445164	1.0321671187348689
8	0.2188958158756762	0.9041872044803384
9	0.17215447630228836	0.8000032266460498
>10	1.202247478828486	12.219323751928984
>50	0.13601932155658586	4.926041544057756
>100	0.083723891267739	7.883732516733512
>500	0.00489462748946122	1.6553577049151478
>1k	0.002576119731295379	3.2536524564025253
>5k	7.728359193886137E-4	2.8745629056585984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	8492	1.1247175623879189	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	6934	0.9183692390011575	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	6185	0.8191684083100894	RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	4950	0.655599615381559	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3906	0.5173276965010848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3320	0.43971529758924766	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3227	0.4273979714820789	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	2332	0.30886026324642335	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	2071	0.27429228352630475	RNA PCR Primer, Index 47 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1217	0.1611847943271429	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1192	0.15787368515854913	No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1150	0.1523110217553117	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1096	0.1451590259511492	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	928	0.12290837233819933	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	788	0.10436616099407443	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	781	0.1034390504268682	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	771	0.1021146067594307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.3244436674374928E-4	0.0
6	0.0	0.0	0.0	1.3244436674374928E-4	0.0
7	0.0	0.0	0.0	1.3244436674374928E-4	0.0
8	0.0	0.0	0.0	1.3244436674374928E-4	0.0
9	0.0	0.0	0.0	1.3244436674374928E-4	0.0
10	0.0	0.0	0.0	1.3244436674374928E-4	0.0
11	0.0	0.0	0.0	1.3244436674374928E-4	0.0
12	0.0	0.0	0.0	1.3244436674374928E-4	0.0
13	0.0	0.0	0.0	1.3244436674374928E-4	0.0
14	0.0	0.0	0.0	1.3244436674374928E-4	0.0
15	0.0	0.0	0.0	1.3244436674374928E-4	0.0
16	0.0	0.0	0.0	1.3244436674374928E-4	0.0
17	0.0	0.0	0.0	2.6488873348749856E-4	0.0
18	0.0	0.0	0.0	2.6488873348749856E-4	0.0
19	0.0	0.0	0.0	3.973331002312479E-4	0.0
20	0.0	0.0	0.0	6.622218337187464E-4	0.0
21	0.0	0.0	0.0	0.0010595549339499943	0.0
22	0.0	0.0	0.0	0.0013244436674374929	0.0
23	0.0	0.0	0.0	0.0018542211344124901	0.0
24	0.0	0.0	0.0	0.001986665501156239	0.0
25	0.0	0.0	0.0	0.0026488873348749858	0.0
26	0.0	0.0	0.0	0.002781331701618735	0.0
27	0.0	0.0	0.0	0.0037084422688249802	0.0
28	0.0	0.0	0.0	0.008476439471599954	0.0
29	0.0	0.0	0.0	0.019866655011562394	0.0
30	0.0	0.0	0.0	0.03642220085453105	0.0
31	0.0	0.0	0.0	0.07774484327858083	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1065	0.0	30.57277	1
CGTTATT	25	0.0054951645	29.599998	2
ACAATCG	25	0.0054951645	29.599998	37
GATTACG	50	2.718698E-7	29.599998	1
GGTATCA	550	0.0	29.263638	1
TATACAC	1120	0.0	28.906248	3
TTATACA	1150	0.0	27.830433	2
GCAACGC	55	6.256978E-7	26.90909	3
CACGCAA	35	8.8665646E-4	26.42857	15
CGTCTTC	325	0.0	25.615385	37
CCGTCTT	915	0.0	24.666666	37
GCCGTCT	930	0.0	24.268816	36
GGGCCGC	40	0.0019305766	23.125002	33
GTATCAA	1555	0.0	23.080387	1
TATTGAG	725	0.0	22.965517	5
AGCAACG	65	2.6808211E-6	22.76923	2
TTATTGA	755	0.0	22.298012	4
GTCTAAT	50	2.700913E-4	22.199999	1
TTACGGG	100	5.329639E-10	22.199999	3
GCTTTAT	750	0.0	21.953335	1
>>END_MODULE