FastQCFastQC Report
Fri 10 Feb 2017
ERR1631110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168307
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT135718.063241576405021No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG122327.267671576345607No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT96075.708021650911727TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC66443.947548230317218No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC35812.127659574468085RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC33812.008829103958837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23421.3915048096632938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21851.2982228903135342No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG14720.8745922629480651No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14080.8365665123851057No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA11320.6725804630823435No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT9880.587022524315685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9260.5501850784578182No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8290.49255230026083285No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8050.47829264379972314No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6910.41055927560945177No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6570.3903580956228796No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6300.37431598210413114No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5860.34817327859209657No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5540.3291604033106169No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5340.3172773562596921No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5310.31549489920205337No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5130.3048001568562211No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4320.25667381629997565No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3890.23112526514048734No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3650.2168656086793776No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3620.21508315162173886No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3590.2133006945641001No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3490.20735917103863774No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3470.20617086633354523No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3260.1936936669300742No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3250.19309951457752797No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT3200.19012875281479677No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3180.18894044810970428No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3090.18359307693678814No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3070.18240477223169566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.16755096341803966No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2780.1651743540078547No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2710.161015287540031No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2690.15982698283493854No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2670.15863867812984606No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2670.15863867812984606No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2560.15210300225183743No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA2470.14675563107892126No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2420.14378486931619006No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2410.14319071696364383No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2340.13903165049582014No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2230.1324959746178115No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2150.12774275579744157No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2140.12714860344489534No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2130.12655445109234909No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2020.12001877521434046No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1980.1176421658041555No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1970.11704801345160927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.11585970874651678No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1890.11229479463123934No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1880.1117006422786931No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1870.11110648992614686No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1870.11110648992614686No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1850.10991818522105437No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1830.10872988051596191No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG1790.10635327110577694No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1780.1057591187532307No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1690.10041174758031454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG200.001837475637.01
TTTACAC200.001837475637.02
GTTCCAG251.2291536E-436.99999619
TCCAGTG251.2291536E-436.99999621
ACTCAGA251.2291536E-436.9999961
CTCAGTT251.2291536E-436.99999615
GTCTCAG251.2291536E-436.99999613
GTGTGGC251.2291536E-436.99999625
CTTATAC15000.036.0133321
TTATACA16250.033.2430762
CGTCTTC5250.033.1238137
CTGGTCA352.3763027E-531.71428531
CAGACAC352.3763027E-531.71428518
TATACAC17500.030.9742873
TGGTCAT303.5865523E-430.83333232
CCGTGTC303.5865523E-430.8333329
GGCTGGT303.5865523E-430.83333229
TCAGAGT303.5865523E-430.8333323
CTGGACC303.5865523E-430.8333324
TGGACCG303.5865523E-430.8333325
Produced by FastQC (version 0.11.5)