##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	168307
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192950976489392	31.0	31.0	34.0	28.0	34.0
2	31.264267083365517	31.0	31.0	34.0	28.0	34.0
3	30.690844706399616	31.0	31.0	34.0	26.0	34.0
4	34.845419382438045	37.0	35.0	37.0	32.0	37.0
5	35.051911091041966	35.0	35.0	37.0	32.0	37.0
6	35.25762446006405	37.0	35.0	37.0	32.0	37.0
7	35.228742714206774	37.0	35.0	37.0	32.0	37.0
8	35.27628678545753	37.0	35.0	37.0	32.0	37.0
9	37.07146464496426	39.0	37.0	39.0	33.0	39.0
10	36.89959419394321	39.0	37.0	39.0	33.0	39.0
11	36.95243216265515	39.0	37.0	39.0	33.0	39.0
12	36.81693571865698	39.0	37.0	39.0	32.0	39.0
13	36.9553375676591	39.0	37.0	39.0	33.0	39.0
14	37.75254148668802	39.0	37.0	41.0	32.0	41.0
15	37.76307580789866	39.0	37.0	41.0	32.0	41.0
16	37.78622398355386	39.0	37.0	41.0	33.0	41.0
17	37.69405907062689	39.0	37.0	41.0	32.0	41.0
18	37.9097185500306	40.0	37.0	41.0	33.0	41.0
19	37.822586107529695	40.0	37.0	41.0	33.0	41.0
20	37.81481459475839	39.0	37.0	41.0	33.0	41.0
21	37.82029267944886	39.0	37.0	41.0	33.0	41.0
22	37.51457752796972	39.0	36.0	41.0	32.0	41.0
23	37.677173260767525	39.0	37.0	41.0	32.0	41.0
24	37.41460545313029	39.0	36.0	41.0	32.0	41.0
25	37.38066152922932	39.0	36.0	40.0	32.0	41.0
26	37.300902517423516	39.0	36.0	40.0	32.0	41.0
27	36.94466064988384	39.0	35.0	40.0	31.0	41.0
28	37.14216283339374	39.0	36.0	40.0	31.0	41.0
29	36.99354156392782	39.0	35.0	40.0	31.0	41.0
30	36.80558740872335	39.0	35.0	40.0	30.0	41.0
31	36.75348024740504	38.0	35.0	40.0	31.0	41.0
32	36.43569192012216	38.0	35.0	40.0	30.0	41.0
33	36.38306190473361	38.0	35.0	40.0	30.0	41.0
34	36.23778571301253	38.0	35.0	40.0	30.0	41.0
35	36.24841509859958	38.0	35.0	40.0	30.0	41.0
36	36.21513662533347	38.0	35.0	40.0	30.0	41.0
37	36.02791327752262	38.0	35.0	40.0	30.0	41.0
38	35.923669247268386	38.0	35.0	40.0	30.0	41.0
39	35.73897104695586	38.0	35.0	40.0	29.0	41.0
40	35.33072896552134	38.0	34.0	40.0	26.0	41.0
41	35.29977362795368	38.0	34.0	40.0	26.0	41.0
42	35.09918779373407	38.0	34.0	40.0	26.0	41.0
43	34.03569073181745	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	9.0
10	15.0
11	16.0
12	10.0
13	8.0
14	13.0
15	7.0
16	13.0
17	5.0
18	16.0
19	27.0
20	39.0
21	58.0
22	131.0
23	252.0
24	455.0
25	703.0
26	1109.0
27	1677.0
28	2387.0
29	3242.0
30	4269.0
31	5276.0
32	6515.0
33	8216.0
34	10667.0
35	13947.0
36	18613.0
37	26231.0
38	36816.0
39	27561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.402775879791093	24.59612493835669	17.643354108860592	27.35774507299162
2	10.906854735691326	20.216033795385812	44.87335642605477	24.003755042868093
3	12.558598275769873	32.37833245200734	37.77739487959503	17.285674392627758
4	8.253370329219818	22.166635968795116	26.350062683073194	43.22993101891187
5	12.527702353437467	37.54151639563417	31.072385581110705	18.858395669817654
6	24.439268717284488	36.962217851901585	15.666015079586707	22.93249835122722
7	19.874396192671725	34.96408349028858	14.781916378997902	30.379603938041793
8	26.778446529259032	28.842531802004668	26.064869553851	18.3141521148853
9	18.518540524161207	11.074405699109366	13.729672562638513	56.677381214090914
10	15.087904840559215	27.13731455019696	27.246044430712924	30.528736178530895
11	28.303041465892687	18.485267992418617	23.697172428954232	29.514518112734468
12	17.21912932914258	27.46469249644994	19.651589060466883	35.6645891139406
13	36.312215178216	14.664868365546294	24.716143713571036	24.306772742666674
14	22.30685592399603	25.989412205077628	20.653329926859847	31.050401944066497
15	29.067715543619695	24.0423749457836	16.827582928814607	30.062326581782102
16	31.17755054751139	20.906438829044543	16.367709007943816	31.548301615500247
17	17.507887372480052	36.74475809086966	16.907199344055805	28.840155192594487
18	27.68571717159714	14.95600301829395	22.123262847059245	35.23501696304967
19	23.293742981575335	27.16405140606154	25.91276655159916	23.62943906076396
20	20.65570653627003	24.692377619469184	20.410321614668433	34.24159422959235
21	37.35792331869738	16.89353383994724	22.188619605839328	23.55992323551605
22	22.473812735061525	38.67753569370258	20.213657185975627	18.634994385260267
23	26.843209135686575	18.771055273993355	21.207079919432942	33.17865567088713
24	27.10107125669164	30.30414658926842	26.688135371672004	15.906646782367934
25	20.26713089770479	30.226906783437407	25.950198149809573	23.555764169048228
26	35.85234125734521	21.09240851539152	27.558568568152246	15.496681659111028
27	20.88326688729524	38.16418806110263	25.42021425133833	15.532330800263805
28	23.678753706025297	18.55597212237162	27.90377108498161	29.86150308662147
29	25.18433576737747	23.12738032286239	30.261367619885092	21.42691628987505
30	17.167438074471058	25.564593273007063	32.921387702234604	24.346580950287272
31	34.26179540957893	23.146987350496413	19.177455483134985	23.413761756789675
32	28.497329285175304	20.217222100090908	33.29689198904383	17.98855662568996
33	23.009144004705686	18.788879844569745	31.823988307081702	26.37798784364287
34	16.112223496348935	37.817203087215624	24.42441490847083	21.646158507964614
35	17.968949598055932	18.247012899047572	48.34261201257226	15.441425490324228
36	31.015941107618815	18.928505647418113	21.456029755149817	28.599523489813254
37	20.67471941155151	25.602024871217477	29.98449262359854	23.73876309363247
38	19.572566797578236	16.77173260767526	32.25534291503027	31.400357679716233
39	29.8169416601805	18.629647014087354	24.53670970310207	27.016701622630073
40	16.69805771595953	17.448472137225426	45.03912493241517	20.814345214399875
41	23.949093026433836	21.783407701402794	22.439946050966388	31.82755322119698
42	24.46362896373888	16.72122965770883	37.659158561438325	21.155982817113962
43	24.844480621721022	16.89531629700488	34.140588329659494	24.11961475161461
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	30.5
4	57.0
5	57.0
6	93.0
7	129.0
8	150.5
9	172.0
10	279.0
11	386.0
12	386.0
13	607.5
14	829.0
15	2133.5
16	3438.0
17	3504.0
18	3570.0
19	3570.0
20	2940.0
21	2310.0
22	1777.5
23	1245.0
24	1226.0
25	1207.0
26	1207.0
27	1248.5
28	1290.0
29	1230.5
30	1171.0
31	1253.0
32	1335.0
33	1335.0
34	1554.5
35	1774.0
36	1796.5
37	1819.0
38	1907.5
39	1996.0
40	1996.0
41	2411.5
42	2827.0
43	3802.0
44	4777.0
45	6687.5
46	8598.0
47	8598.0
48	12217.0
49	15836.0
50	27369.0
51	38902.0
52	26664.5
53	14427.0
54	14427.0
55	22044.5
56	29662.0
57	23813.5
58	17965.0
59	11504.0
60	5043.0
61	5043.0
62	4212.0
63	3381.0
64	2659.5
65	1938.0
66	1547.0
67	1156.0
68	1156.0
69	942.0
70	728.0
71	492.0
72	256.0
73	145.0
74	34.0
75	34.0
76	26.0
77	18.0
78	15.0
79	12.0
80	9.0
81	6.0
82	6.0
83	7.0
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	168307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.892262353912788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65141365537629	13.536573048060985
2	10.906689310312293	4.12104071726072
3	4.607352894927194	2.6112995894407245
4	2.638613705695506	1.993975295145181
5	1.6542441110796617	1.562620687196611
6	1.2296757555744253	1.3938814190734787
7	1.0063842500864861	1.3309012697035774
8	0.7233386797496619	1.0932403286850814
9	0.5094820266062836	0.8662741300124177
>10	4.236248702707803	15.78128063598068
>50	0.4685976664465201	6.2106745411658455
>100	0.2956253734629053	10.40360769308466
>500	0.03773940937824323	4.898191994391202
>1k	0.022014655470641884	9.209955616819265
>5k	0.006289901563040538	9.655569881228946
>10k+	0.006289901563040538	15.330913152750627
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	13571	8.063241576405021	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	12232	7.267671576345607	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	9607	5.708021650911727	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	6644	3.947548230317218	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3581	2.127659574468085	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3381	2.008829103958837	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2342	1.3915048096632938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2185	1.2982228903135342	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1472	0.8745922629480651	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1408	0.8365665123851057	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1132	0.6725804630823435	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	988	0.587022524315685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.5501850784578182	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	829	0.49255230026083285	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	805	0.47829264379972314	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	691	0.41055927560945177	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	657	0.3903580956228796	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	630	0.37431598210413114	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	586	0.34817327859209657	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	554	0.3291604033106169	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	534	0.3172773562596921	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	531	0.31549489920205337	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	513	0.3048001568562211	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	432	0.25667381629997565	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	389	0.23112526514048734	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	365	0.2168656086793776	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	362	0.21508315162173886	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	359	0.2133006945641001	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	349	0.20735917103863774	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	347	0.20617086633354523	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	326	0.1936936669300742	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	325	0.19309951457752797	No Hit
ATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT	320	0.19012875281479677	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	318	0.18894044810970428	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	309	0.18359307693678814	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	307	0.18240477223169566	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.16755096341803966	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	278	0.1651743540078547	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	271	0.161015287540031	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	269	0.15982698283493854	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	267	0.15863867812984606	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	267	0.15863867812984606	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	256	0.15210300225183743	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	247	0.14675563107892126	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	242	0.14378486931619006	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	241	0.14319071696364383	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	234	0.13903165049582014	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	223	0.1324959746178115	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	215	0.12774275579744157	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	214	0.12714860344489534	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	213	0.12655445109234909	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	202	0.12001877521434046	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	198	0.1176421658041555	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	197	0.11704801345160927	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.11585970874651678	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	189	0.11229479463123934	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	188	0.1117006422786931	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	187	0.11110648992614686	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	187	0.11110648992614686	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	185	0.10991818522105437	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	183	0.10872988051596191	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	179	0.10635327110577694	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	178	0.1057591187532307	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	169	0.10041174758031454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.9415235254624E-4	0.0
10	0.0	0.0	0.0	5.9415235254624E-4	0.0
11	0.0	0.0	0.0	5.9415235254624E-4	0.0
12	0.0	0.0	0.0	5.9415235254624E-4	0.0
13	0.0	0.0	0.0	5.9415235254624E-4	0.0
14	0.0	0.0	0.0	5.9415235254624E-4	0.0
15	0.0	0.0	0.0	5.9415235254624E-4	0.0
16	0.0	0.0	0.0	5.9415235254624E-4	0.0
17	0.0	0.0	0.0	0.00118830470509248	0.0
18	0.0	0.0	0.0	0.00118830470509248	0.0
19	0.0	0.0	0.0	0.00118830470509248	0.0
20	0.0	0.0	0.0	0.00118830470509248	0.0
21	0.0	0.0	0.0	0.00118830470509248	0.0
22	0.0	0.0	0.0	0.0035649141152774393	0.0
23	0.0	0.0	0.0	0.0035649141152774393	0.0
24	0.0	0.0	0.0	0.0035649141152774393	0.0
25	0.0	0.0	0.0	0.005347371172916159	0.0
26	0.0	0.0	0.0	0.005347371172916159	0.0
27	0.0	0.0	0.0	0.005347371172916159	0.0
28	0.0	0.0	0.0	0.01901287528147968	0.0
29	0.0	0.0	0.0	0.052285407024069114	0.0
30	0.0	0.0	0.0	0.09149946229212094	0.0
31	0.0	0.0	0.0	0.14200241225855134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	20	0.0018374756	37.0	1
TTTACAC	20	0.0018374756	37.0	2
GTTCCAG	25	1.2291536E-4	36.999996	19
TCCAGTG	25	1.2291536E-4	36.999996	21
ACTCAGA	25	1.2291536E-4	36.999996	1
CTCAGTT	25	1.2291536E-4	36.999996	15
GTCTCAG	25	1.2291536E-4	36.999996	13
GTGTGGC	25	1.2291536E-4	36.999996	25
CTTATAC	1500	0.0	36.013332	1
TTATACA	1625	0.0	33.243076	2
CGTCTTC	525	0.0	33.12381	37
CTGGTCA	35	2.3763027E-5	31.714285	31
CAGACAC	35	2.3763027E-5	31.714285	18
TATACAC	1750	0.0	30.974287	3
TGGTCAT	30	3.5865523E-4	30.833332	32
CCGTGTC	30	3.5865523E-4	30.833332	9
GGCTGGT	30	3.5865523E-4	30.833332	29
TCAGAGT	30	3.5865523E-4	30.833332	3
CTGGACC	30	3.5865523E-4	30.833332	4
TGGACCG	30	3.5865523E-4	30.833332	5
>>END_MODULE