FastQCFastQC Report
Fri 10 Feb 2017
ERR1631109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418647
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT120642.8816640272114693No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG107662.5716176157956463No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT74661.7833640274503342Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC58761.403569116702138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58671.401419334188469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56881.3586625486388293No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC29120.6955740755338029No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC22780.5441338406820065Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22020.5259801216776903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21190.506154349607187No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14700.3511311438992755No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG14340.34253201384459936No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12010.28687653321294554No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11770.2811437798431614No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11000.26275119611510417No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA10340.2469861243481979No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9420.22501056976402556No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT9090.21712803388057242No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8970.21426165719568036No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8930.21330619830071634No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8740.20876776854963727No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8340.19921317959999715No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.16051709435395453No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT6570.15693412349783947No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6470.15454547626042944No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6370.15215682902301939No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6260.14952931706186834No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5980.14284110479712023No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5930.14164678117841523No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5830.1392581339410052No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5680.13567516308489014No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5620.13424197474244412No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5600.1337642452949621No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5510.13161446278129307No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5170.12349306217409894No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5060.1208655502129479No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5000.11943236187050188No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4710.11250528488201278No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4610.11011663764460274No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4600.10987777292086173No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.10963890819712073No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4540.1084445845784157No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4400.10510047844604165No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4300.10271183120863163No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4300.10271183120863163No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4240.1012786428661856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAC200.001840599837.01
CTTATAC14200.034.5246471
GATTACG651.8189894E-1234.1538471
TTACGGG603.45608E-1133.9166643
CGTCTTC3400.033.73529437
TTATACA15150.032.2376252
TATACAC16150.030.928793
ACGTTAA250.005492402229.59999820
GGTATCA6650.028.0977461
GACGTAT405.9312486E-527.7528
ATTACGG803.274181E-1127.752
AAACACG405.9312486E-527.7515
ATACGGT405.9312486E-527.7533
TATACGG405.9312486E-527.7532
CGGGTAT358.8599394E-426.4285717
TAGCAAC358.8599394E-426.428571
GTATCAA21650.025.7205541
CCGTCTT11550.025.3073637
GCCGTCT11550.025.3073636
TGGACGT451.3212497E-424.66666626
Produced by FastQC (version 0.11.5)