##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	418647
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3886448487628	31.0	31.0	34.0	28.0	34.0
2	31.3571600895265	31.0	31.0	34.0	28.0	34.0
3	30.796580412614922	31.0	31.0	34.0	26.0	34.0
4	34.98719446216025	37.0	35.0	37.0	32.0	37.0
5	35.21334919395099	37.0	35.0	37.0	33.0	37.0
6	35.428743069937205	37.0	35.0	37.0	33.0	37.0
7	35.43123681765306	37.0	35.0	37.0	33.0	37.0
8	35.45052036680067	37.0	35.0	37.0	33.0	37.0
9	37.23531758259345	39.0	37.0	39.0	34.0	39.0
10	37.07285851803548	39.0	37.0	39.0	33.0	39.0
11	37.13177211349896	39.0	37.0	39.0	33.0	39.0
12	37.022694537402636	39.0	37.0	39.0	33.0	39.0
13	37.1009155684861	39.0	37.0	39.0	33.0	39.0
14	38.07819953325833	40.0	37.0	41.0	33.0	41.0
15	38.072791635912836	40.0	37.0	41.0	33.0	41.0
16	38.10387032511878	40.0	37.0	41.0	33.0	41.0
17	38.14386105716749	40.0	37.0	41.0	33.0	41.0
18	38.2223782805084	40.0	38.0	41.0	33.0	41.0
19	38.23482790990978	40.0	38.0	41.0	33.0	41.0
20	38.23287877376406	40.0	38.0	41.0	34.0	41.0
21	38.177779847938716	40.0	37.0	41.0	33.0	41.0
22	38.03967781926062	40.0	37.0	41.0	33.0	41.0
23	38.08670550607075	40.0	37.0	41.0	33.0	41.0
24	38.01275776489501	40.0	37.0	41.0	33.0	41.0
25	37.92194139692868	40.0	37.0	41.0	33.0	41.0
26	37.841326941313326	40.0	37.0	41.0	33.0	41.0
27	37.7059623023693	40.0	37.0	41.0	33.0	41.0
28	37.69457323234133	40.0	37.0	41.0	33.0	41.0
29	37.605834987471546	39.0	37.0	41.0	33.0	41.0
30	37.42275711996025	39.0	36.0	41.0	32.0	41.0
31	37.35005386399521	39.0	36.0	41.0	32.0	41.0
32	37.12140777313584	39.0	35.0	41.0	31.0	41.0
33	37.066280183543654	39.0	35.0	41.0	31.0	41.0
34	36.96490360614074	39.0	35.0	41.0	31.0	41.0
35	36.878597004158635	39.0	35.0	41.0	31.0	41.0
36	36.781553432844376	39.0	35.0	41.0	31.0	41.0
37	36.688150159919935	39.0	35.0	40.0	31.0	41.0
38	36.576022281301434	39.0	35.0	40.0	30.0	41.0
39	36.4191717604569	39.0	35.0	40.0	30.0	41.0
40	36.19206395841843	39.0	35.0	40.0	30.0	41.0
41	36.079114385150255	39.0	35.0	40.0	29.0	41.0
42	35.901387087450765	38.0	35.0	40.0	28.0	41.0
43	35.08823424030269	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	15.0
11	15.0
12	10.0
13	8.0
14	6.0
15	14.0
16	7.0
17	6.0
18	12.0
19	30.0
20	65.0
21	144.0
22	242.0
23	462.0
24	772.0
25	1341.0
26	2044.0
27	3281.0
28	4674.0
29	6459.0
30	8289.0
31	10917.0
32	13793.0
33	17593.0
34	23228.0
35	30965.0
36	42288.0
37	62192.0
38	94522.0
39	95243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67949131368432	21.499974919204007	15.945892362778189	22.874641404333484
2	13.72373383781563	23.62825960773635	41.22805131769725	21.41995523675077
3	15.198723506916329	30.176974873819713	34.505920262177916	20.11838135708604
4	10.661488079455962	19.114432923202603	35.00992482927144	35.214154168069996
5	13.819757456759513	37.50773324543111	31.41047230721825	17.262036990591117
6	26.736606257778035	39.3746999261908	16.008952649845813	17.879741166185354
7	23.600312435058655	32.87399646957938	19.676003888717702	23.84968720664426
8	26.71534729736508	31.355772285481564	22.6498696992932	19.279010717860153
9	24.17502096037951	12.42908703513939	18.156346516277434	45.23954548820367
10	16.187862327927824	28.11342252542118	29.491194251959286	26.20752089469171
11	32.50757798336068	22.511328159523416	22.709824744952193	22.271269112163708
12	21.666463631651485	27.165607301616873	26.610007954195304	24.557921112536338
13	32.93180173272471	20.385670982952224	24.961841360382376	21.720685923940692
14	22.765241360860102	22.18288916437954	27.381063282431263	27.6708061923291
15	26.06467978989459	26.141833095662932	22.791277615747873	25.002209498694604
16	24.747340838462954	25.466801386370857	21.730957107061556	28.054900668104633
17	20.641256237355098	29.16848801018519	23.953115632024115	26.237140120435594
18	25.656221112297473	21.33372507148027	26.78437920252623	26.225674613696025
19	25.746511977871574	24.195324461897492	28.16358411740679	21.894579442824146
20	23.855658824737784	23.617749559891745	26.137294665911853	26.38929694945861
21	27.128822134160764	22.204625854239968	26.476721438347823	24.18983057325145
22	22.15709177421551	30.315755278313233	27.13789899366292	20.38925395380834
23	23.474908455094624	22.33409053450759	27.780206235802478	26.410794774595303
24	24.624564370460078	24.10145062546728	30.340119480134813	20.93386552393783
25	23.258497015385277	26.890196275143495	26.330536227418328	23.5207704820529
26	24.74447446177806	23.140975571304704	29.026124634835554	23.088425332081684
27	22.885629181625568	29.406636139754973	28.107928636775135	19.59980604184432
28	19.0905464508285	23.53247485351621	29.270960976670086	28.106017718985203
29	23.39942720239247	22.82877937737521	30.915783464350632	22.856009955881685
30	21.72498548896803	25.72238664077373	29.302729984927634	23.2498978853306
31	23.7782666542457	21.70515971689753	27.67104505705284	26.84552857180393
32	26.23116850234207	23.5487176547306	29.0182420989521	21.201871743975236
33	19.90125332320547	22.85051606723564	30.971200080258548	26.277030529300344
34	19.660238816950795	25.949785857775165	31.21245345123696	23.177521874037076
35	23.597446058373762	23.07146593669607	32.86898031038083	20.462107694549346
36	22.92169775491046	23.75414131714786	26.131800777265813	27.192360150675864
37	22.321191839425577	25.867855257532003	29.451064978370802	22.35988792467162
38	21.127823679615524	23.063105671365136	30.04631586993338	25.76275477908596
39	24.91024658005432	22.314264762437087	28.062544339264345	24.712944318244247
40	20.92216115247452	22.84741082582701	34.37669444663404	21.853733575064435
41	24.161644535850012	23.89459377470757	26.0391212644543	25.904640424988116
42	22.981413935845712	22.918353648778087	31.866465064839826	22.23376735053637
43	23.431912804821245	22.632671439183845	30.47913874935208	23.45627700664283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	17.0
2	22.0
3	104.0
4	186.0
5	186.0
6	275.5
7	365.0
8	395.0
9	425.0
10	681.5
11	938.0
12	938.0
13	1489.0
14	2040.0
15	5311.0
16	8582.0
17	8744.5
18	8907.0
19	8907.0
20	7292.5
21	5678.0
22	4865.0
23	4052.0
24	4438.0
25	4824.0
26	4824.0
27	5445.5
28	6067.0
29	6869.0
30	7671.0
31	8618.0
32	9565.0
33	9565.0
34	10994.0
35	12423.0
36	13396.0
37	14369.0
38	15003.0
39	15637.0
40	15637.0
41	16680.0
42	17723.0
43	19136.0
44	20549.0
45	23695.0
46	26841.0
47	26841.0
48	30933.5
49	35026.0
50	34825.0
51	34624.0
52	46907.0
53	59190.0
54	59190.0
55	45945.0
56	32700.0
57	36491.5
58	40283.0
59	32256.0
60	24229.0
61	24229.0
62	17788.5
63	11348.0
64	8907.5
65	6467.0
66	5305.5
67	4144.0
68	4144.0
69	3147.5
70	2151.0
71	1534.0
72	917.0
73	621.0
74	325.0
75	325.0
76	255.0
77	185.0
78	140.0
79	95.0
80	71.0
81	47.0
82	47.0
83	36.5
84	26.0
85	17.5
86	9.0
87	6.0
88	3.0
89	3.0
90	2.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	418647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.979134135850764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.92421067246929	32.34245815713263
2	11.767871249568824	9.644743488590052
3	3.764319279328898	4.62775634033364
4	1.6488067374650646	2.702666898347014
5	0.8748991176102054	1.792630414794304
6	0.5263802949945783	1.294236522903091
7	0.3625554819477185	1.0400046808496504
8	0.24504898905362466	0.8033516313826489
9	0.1930397048154769	0.7119539961460618
>10	1.3717908515863488	11.287652716032829
>50	0.1699133396649343	4.989039001716706
>100	0.13007159105384625	9.968474001312584
>500	0.011718161356202366	3.271603322459783
>1k	0.005859080678101183	4.064173597481047
>5k	0.0023436322712404732	5.977771020055866
>10k+	0.0011718161356202366	5.481484210462121
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	12064	2.8816640272114693	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	10766	2.5716176157956463	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	7466	1.7833640274503342	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	5876	1.403569116702138	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5867	1.401419334188469	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5688	1.3586625486388293	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2912	0.6955740755338029	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	2278	0.5441338406820065	Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2202	0.5259801216776903	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2119	0.506154349607187	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1470	0.3511311438992755	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1434	0.34253201384459936	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1201	0.28687653321294554	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1177	0.2811437798431614	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1100	0.26275119611510417	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	1034	0.2469861243481979	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	942	0.22501056976402556	No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT	909	0.21712803388057242	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	897	0.21426165719568036	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	893	0.21330619830071634	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	874	0.20876776854963727	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	834	0.19921317959999715	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.16051709435395453	No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT	657	0.15693412349783947	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	647	0.15454547626042944	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	637	0.15215682902301939	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	626	0.14952931706186834	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	598	0.14284110479712023	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	593	0.14164678117841523	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	583	0.1392581339410052	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	568	0.13567516308489014	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	562	0.13424197474244412	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	560	0.1337642452949621	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	551	0.13161446278129307	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	517	0.12349306217409894	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	506	0.1208655502129479	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	500	0.11943236187050188	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	471	0.11250528488201278	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	461	0.11011663764460274	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	460	0.10987777292086173	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	459	0.10963890819712073	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	454	0.1084445845784157	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	440	0.10510047844604165	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	430	0.10271183120863163	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	430	0.10271183120863163	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	424	0.1012786428661856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	4.7772944748200754E-4	0.0
14	0.0	0.0	0.0	4.7772944748200754E-4	0.0
15	0.0	0.0	0.0	4.7772944748200754E-4	0.0
16	0.0	0.0	0.0	4.7772944748200754E-4	0.0
17	0.0	0.0	0.0	4.7772944748200754E-4	0.0
18	0.0	0.0	0.0	4.7772944748200754E-4	0.0
19	0.0	0.0	0.0	4.7772944748200754E-4	0.0
20	0.0	0.0	0.0	7.165941712230112E-4	0.0
21	0.0	0.0	0.0	0.0021497825136690338	0.0
22	0.0	0.0	0.0	0.0042995650273380676	0.0
23	0.0	0.0	0.0	0.005493888646043087	0.0
24	0.0	0.0	0.0	0.007882535883453124	0.0
25	0.0	0.0	0.0	0.008360265330935132	0.0
26	0.0	0.0	0.0	0.009076859502158143	0.0
27	0.0	0.0	0.0	0.011943236187050187	0.0
28	0.0	0.0	0.0	0.027469443230215433	0.0
29	0.0	0.0	0.0	0.05947731621150994	0.0
30	0.0	0.0	0.0	0.09339610698273247	0.0
31	0.0	0.0	0.0	0.15072364068057337	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTTAC	20	0.0018405998	37.0	1
CTTATAC	1420	0.0	34.524647	1
GATTACG	65	1.8189894E-12	34.153847	1
TTACGGG	60	3.45608E-11	33.916664	3
CGTCTTC	340	0.0	33.735294	37
TTATACA	1515	0.0	32.237625	2
TATACAC	1615	0.0	30.92879	3
ACGTTAA	25	0.0054924022	29.599998	20
GGTATCA	665	0.0	28.097746	1
GACGTAT	40	5.9312486E-5	27.75	28
ATTACGG	80	3.274181E-11	27.75	2
AAACACG	40	5.9312486E-5	27.75	15
ATACGGT	40	5.9312486E-5	27.75	33
TATACGG	40	5.9312486E-5	27.75	32
CGGGTAT	35	8.8599394E-4	26.42857	17
TAGCAAC	35	8.8599394E-4	26.42857	1
GTATCAA	2165	0.0	25.720554	1
CCGTCTT	1155	0.0	25.30736	37
GCCGTCT	1155	0.0	25.30736	36
TGGACGT	45	1.3212497E-4	24.666666	26
>>END_MODULE