FastQCFastQC Report
Fri 10 Feb 2017
ERR1631108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416288
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT100482.413713582904143RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG95012.282314167115074No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT94732.2755880544238605No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC55211.3262452917211163No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC32480.7802290721807978RNA PCR Primer, Index 46 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC32250.7747040510415866No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG14260.34255131063110156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.3197305711430548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13040.31324467676224155No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA9100.21859866246444767No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT7820.18785071873318474No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7600.1825659159043739No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT6650.15974517641632716No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6470.15542124682911831No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC6140.14749404258590207No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5770.13860596510108386No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5750.13812552848028287No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA5360.1287570143746637No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5220.1253939580290568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4630.11122107771542777No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4600.1105004227842263No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4590.11026020447382581No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4270.10257321854101008No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4260.1023330002306096No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT4230.10161234529940812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC200.001840587837.03
CTTATAC11400.034.8903541
TTACGGG401.5968235E-632.3753
GATTACG401.5968235E-632.3751
CGTCTTC3650.031.42465837
TTATACA13150.030.8098872
TATACAC13800.029.6268123
GTGTCGG250.005492366429.615
GTCGGTT250.005492366429.617
ATTACGG454.000185E-628.7777792
CCGTCTT14750.028.09491537
GCCGTCT15150.027.2310236
GCTCTAG451.3212318E-424.6666681
GTATCAA5300.023.3867931
GGTATCA2100.022.9047641
TGCCGTC18750.022.10133435
ATGCCGT19900.020.91708634
ACGGGAC759.242509E-619.7333323
TATGCCG21550.019.40139233
GCCTTAA609.223331E-418.51
Produced by FastQC (version 0.11.5)