FastQCFastQC Report
Fri 10 Feb 2017
ERR1631107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269723
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT171366.353184563422475No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG129574.803817249548611No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT118304.38598117327777RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC68582.542608528008364No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC51581.912332281637087RNA PCR Primer, Index 27 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41741.5475135602080654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37431.3877199942162886No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC34851.2920663050611183RNA PCR Primer, Index 27 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24790.919091067502586No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15260.5657656188015112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14800.5487110850761707No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG14290.5298027976850324No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA13490.5001427390322664No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12550.46529217011526636No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT11010.40819655720869186No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10820.40115229327866No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10610.3933665278823089No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9860.3655602228953408No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9810.3637064692295429No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9710.35999896189794717No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8930.3310804047115003No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8870.32885590031254286No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT7570.28065830500179817No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6940.2573010088127449No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6430.2383927214216066No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6240.23134845749157468No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6090.22578719649418108No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5990.2220796891625853No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5830.21614767743203211No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5760.2135524222999151No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5610.20799116130252146No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5480.20317140177144702No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5430.20131764810564912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.20094689737248952No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5280.1957563871082555No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5130.1901951261108619No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4990.18500461584662783No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4980.18463386511346824No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4950.18352161291398952No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4830.17907260411607465No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4520.16757933138812783No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4410.1635010733233725No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4240.15719831085965974No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4020.14904179473014909No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4010.1486710439969895No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3880.14385128446591502No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3860.14310978299959587No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3840.14236828153327674No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT3820.14162678006695759No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3700.13717777126904268No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT3680.13643626980272355RNA PCR Primer, Index 27 (100% over 23bp)
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3650.1353240176032448No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3580.13272876247112778No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3560.13198726100480865No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3550.13161651027164906No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3470.12865050440637246No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3410.12642600000741502No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3350.12420149560845757No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3310.12271849267581926No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3300.1223477419426597No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3300.1223477419426597No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3220.1193817360773831No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3200.11864023461106395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.1164157302121065No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3000.11122521994787246No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2950.10937146628207457No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2920.10825921408259584No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2920.10825921408259584No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2910.10788846334943626No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2900.1075177126162767No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2890.10714696188311712No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2820.1045517067510001No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2720.10084419941940437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG251.2311217E-437.01
TCGCCGA200.001839439637.01
TTCTACA251.2311217E-437.02
AGCAACG200.001839439637.02
TGATACA308.288402E-637.02
TCAGAGT251.2311217E-437.03
TTACGGG501.7826096E-1037.03
GATTACG501.7826096E-1037.01
CTTATAC20350.035.9090961
ATTACGG554.965841E-1033.6363642
TTATACA21950.033.207292
CGTCTTC6700.032.3059737
CGTTACT352.3815843E-531.7142871
TATACAC23600.031.1207623
TTACTTT303.592242E-430.8333323
CGTTATT303.592242E-430.83333210
ACTCAGA303.592242E-430.8333321
GACAGAC250.00548897929.616
TTTACAC250.00548897929.62
TTAGCGG250.00548897929.63
Produced by FastQC (version 0.11.5)