FastQCFastQC Report
Fri 10 Feb 2017
ERR1631106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336443
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT202206.009933331946273No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG198315.894311963690729No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT148574.415904031292076Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC108233.216889636580342No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC57751.7164868937680382Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC48431.4394711734231356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46051.3687311074981496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44571.3247414866708476No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27520.8179691656536173No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG25590.7606043222774734No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA18070.5370894921279384No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18050.5364950377924343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17280.5136085458755272No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17190.5109335013657589No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT15670.4657549718674486No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15110.4491102504733342No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT13910.4134429903430893No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12380.36796723367702705No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11830.3516197394506647No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT11730.3486474677731443No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10790.3207081140044525No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10410.30941348162987486No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10100.3001994394295616No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8620.25620981860225955No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8060.23956509720814523No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7710.22916214633682377No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7540.22410928448503906No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7410.22024533130426252No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7220.21459801511697374No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7120.2116257434394533No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7120.2116257434394533No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7090.2107340619361972No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6770.20122279256813191No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6740.20033111106487578No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6380.18963093302580228No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6300.18725311568378594No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6220.18487529834176963No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6160.1830919353352574No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6110.1816057994964972No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5680.1688250312831594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.16347494226362266No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT5480.16288048792811857Illumina PCR Primer Index 10 (95% over 21bp)
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5250.15604426306982164No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5230.15544980873431755No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5210.15485535439881348No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5070.15069417405028487No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4960.14742467520501246No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4810.14296626768873183No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4750.14118290468221958No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4720.14029122317896345No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4600.13672449716593896No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4440.13196886248190628No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4380.13018549947539404No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4380.13018549947539404No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4190.12453818328810526No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4110.12216036594608895No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4100.12186313877833689No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4070.12097145727508077No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4060.12067423010732874No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4020.11948532143632057No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4020.11948532143632057No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3910.1162158225910481No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3850.11443245958453586No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3800.11294632374577566No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3790.1126490965780236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.11146018790701545No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3750.11146018790701545No Hit
CTGATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT3710.11027127923600728No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3690.1096768249005032No Hit
TCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT3690.1096768249005032Illumina PCR Primer Index 10 (95% over 24bp)
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3680.10937959773275117No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3650.10848791622949505No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3650.10848791622949505No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3640.10819068906174299No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3610.10729900755848687No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3570.10611009888747872No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3520.1046239630487185No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3510.10432673588096646No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3510.10432673588096646No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC3480.10343505437771035No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3450.10254337287445422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG551.2732926E-1137.02
TCAGAGT403.819514E-837.03
GTATACA200.001840085837.02
TTGTTAG200.001840085837.03
GATTACG551.2732926E-1137.01
TAGCAAC251.2317704E-436.9999961
TTACGGG501.7826096E-1036.9999963
CTTATAC20000.035.7051
CAGACAC651.8189894E-1234.15384718
CGTCTTC5800.033.81034537
TTATACA21650.033.0692862
CTCAGAG451.0761141E-732.888892
GCCGAGC401.5956157E-632.3753
CGCCGAG401.5956157E-632.3752
TATACAC23200.031.0991383
GTTTGTG303.5941158E-430.8333321
TCGCCGA453.9971837E-628.7777791
CCGTCTT21900.027.36986237
ACTCAGA556.239643E-726.909091
GGTATCA5450.026.4770641
Produced by FastQC (version 0.11.5)