FastQCFastQC Report
Fri 10 Feb 2017
ERR1631105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196374
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT130976.669416521535437No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG114405.825618462729282No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT99045.043437522278917RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC59833.0467373481214417No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC36751.871429007913471RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC31441.6010266124843413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31211.58931426767291No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27771.4141383278845467No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16620.846344220721684No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG13600.6925560410237608No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA12070.6146434864085877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11360.5784879872080826No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10200.5194170307678205No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT9370.4771507429700469No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9300.473586116288307No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7540.38396121686170265RNA PCR Primer, Index 26 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7500.38192428732927985No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7420.37785042826443416No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7260.3697027101347429No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6910.3518795767260432No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC6680.3401672319146119No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6440.327945654720075No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5540.2821147402405614No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5140.2617454449163331No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4730.24086691720899917No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4520.23017303716377932No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4200.21387760090439673No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4130.21031297422265677No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4120.20980374183955106No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4100.20878527707333966No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4000.20369295324228257No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3970.20216525609296548No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3960.20165602370985974No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3950.20114679132675403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.19299907319706275No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3670.18688828459979429No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3530.17975903123631437No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3420.17415747502215162No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3420.17415747502215162No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3290.16753745404177742RNA PCR Primer, Index 26 (95% over 23bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3110.1583712711458747No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3070.1563343416134519No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2940.14971432063307769No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2900.14767739110065486No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2780.1415666025033864No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.13647427867232934No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2650.1349465815230122No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2650.1349465815230122No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2580.13138195484127227No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2550.12985425769195513No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2540.12934502530884945No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2540.12934502530884945No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2500.1273080957764266No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2470.1257803986271095No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2420.12323423671158096No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2410.12272500432847526No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2330.1186511452636296No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2280.11610498334810107No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA2220.11304958904946683No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2190.11152189190014972No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2180.11101265951704402No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2180.11101265951704402No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2170.1105034271339383No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2150.10948496236772688No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2090.10642956806909264No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2040.10388340615356412No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2040.10388340615356412No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2010.10235570900424701No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG1990.10133724423803558No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1990.10133724423803558No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG1980.10082801185492987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACA308.276904E-637.03
TGATACA251.2299007E-437.02
ATTACGG200.001838221837.02
GATTACG200.001838221837.01
TTTACAC308.276904E-637.02
TTACGGG200.001838221837.03
CTTATAC14100.035.6879431
CGTCTTC3550.035.4366237
TTATACA15200.033.1052632
TTATGAG352.378309E-531.7142853
ATAACAC352.378309E-531.7142854
CTTATAA352.378309E-531.7142851
TATACAC16000.031.6812523
GATACAC303.588713E-430.8333323
ACTCAGA303.588713E-430.8333321
TCGCCGA303.588713E-430.8333321
TCAGAGT303.588713E-430.8333323
GAGGTCG250.00548538729.626
CTTTATG250.00548538729.61
GTCGCTT250.00548538729.629
Produced by FastQC (version 0.11.5)