##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	196374
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13778300589691	31.0	31.0	34.0	28.0	34.0
2	31.17030258588204	31.0	31.0	34.0	28.0	34.0
3	30.61850346787253	31.0	30.0	34.0	26.0	34.0
4	34.776640492122176	35.0	35.0	37.0	32.0	37.0
5	34.9853748459572	35.0	35.0	37.0	32.0	37.0
6	35.20346889099372	36.0	35.0	37.0	32.0	37.0
7	35.17839938077342	37.0	35.0	37.0	32.0	37.0
8	35.198096489351954	37.0	35.0	37.0	32.0	37.0
9	37.013851120820476	39.0	37.0	39.0	33.0	39.0
10	36.84106857323271	39.0	37.0	39.0	33.0	39.0
11	36.878614276839095	39.0	37.0	39.0	33.0	39.0
12	36.7410807948099	39.0	35.0	39.0	32.0	39.0
13	36.862726226486195	39.0	37.0	39.0	33.0	39.0
14	37.65346736329656	39.0	37.0	41.0	32.0	41.0
15	37.63854176214774	39.0	37.0	41.0	32.0	41.0
16	37.61949647101959	39.0	37.0	40.0	32.0	41.0
17	37.60407691445914	39.0	37.0	40.0	32.0	41.0
18	37.83339953354314	39.0	37.0	41.0	33.0	41.0
19	37.767698371474836	39.0	37.0	41.0	32.0	41.0
20	37.84882927475124	39.0	37.0	41.0	33.0	41.0
21	37.780699074215526	39.0	37.0	41.0	33.0	41.0
22	37.5079694867956	39.0	36.0	40.0	32.0	41.0
23	37.583982604621795	39.0	36.0	41.0	32.0	41.0
24	37.36725839469584	39.0	36.0	40.0	32.0	41.0
25	37.31145671015511	39.0	36.0	40.0	31.0	41.0
26	37.17130577367676	39.0	36.0	40.0	31.0	41.0
27	36.99147544990681	39.0	35.0	40.0	31.0	41.0
28	37.08591259535376	39.0	35.0	40.0	31.0	41.0
29	37.013138195484125	39.0	35.0	40.0	31.0	41.0
30	36.759199283000804	39.0	35.0	40.0	30.0	41.0
31	36.618732622444924	38.0	35.0	40.0	30.0	41.0
32	36.366479269149686	38.0	35.0	40.0	30.0	41.0
33	36.17700917636754	38.0	35.0	40.0	30.0	41.0
34	36.02844572092028	38.0	35.0	40.0	30.0	41.0
35	35.98846079419882	38.0	35.0	40.0	30.0	41.0
36	35.87165307016204	38.0	35.0	40.0	29.0	41.0
37	35.79492193467567	38.0	35.0	40.0	29.0	41.0
38	35.708158921242116	38.0	35.0	40.0	29.0	41.0
39	35.47539898357216	38.0	34.0	40.0	27.0	41.0
40	35.05446749569698	38.0	34.0	40.0	26.0	41.0
41	34.99570207868659	38.0	34.0	40.0	26.0	41.0
42	34.75879698941815	38.0	33.0	40.0	24.0	41.0
43	33.63657612514895	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	19.0
11	24.0
12	15.0
13	10.0
14	8.0
15	18.0
16	16.0
17	13.0
18	14.0
19	41.0
20	49.0
21	117.0
22	183.0
23	321.0
24	547.0
25	911.0
26	1458.0
27	2056.0
28	2954.0
29	3976.0
30	5032.0
31	6519.0
32	7971.0
33	10003.0
34	12732.0
35	16539.0
36	22051.0
37	31315.0
38	42496.0
39	28953.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51869392078381	22.648110238626295	17.722305396844796	26.1108904437451
2	11.961868679153044	21.204436432521618	43.29442797926406	23.539266909061283
3	14.200454235285731	31.038732215059024	36.86536914255451	17.895444407100737
4	9.186042958843839	21.163697841873162	27.99861488791795	41.65164431136505
5	13.377534704186909	37.298725900577466	30.271319013718724	19.052420381516903
6	25.70401376964364	37.0868852292055	15.200586635705339	22.008514365445528
7	21.819079918930203	33.78705938668052	15.472516728283786	28.921343966105496
8	27.67881695132757	28.90046543839816	24.34792793343314	19.07278967684113
9	20.227728721724873	11.102793648853718	14.51210445374642	54.157373175674984
10	15.726114455070428	27.14157678715105	27.096764337437744	30.035544420340777
11	30.014665892633445	19.25560410237608	23.078411602350617	27.651318402639863
12	18.84516280159288	26.93279151007771	21.075600639595873	33.14644504873354
13	35.778667237006935	15.739354497031174	24.49764225406622	23.984336011895667
14	22.930224978866857	25.08224102987157	22.343080041145978	29.644453950115597
15	29.053235153329872	24.423294326132787	18.33134732703922	28.19212319349812
16	29.839999185228187	21.343456873109474	17.697862242455724	31.11868169920662
17	18.4718954647764	34.41748907696538	18.654709890311345	28.455905567946875
18	27.193009257844725	15.711346715960362	23.46491898112785	33.63072504506707
19	24.24302606251337	23.998594518622628	26.62215975638323	25.136219662480773
20	20.11773452697404	18.527911026918023	22.064529927587156	39.28982451852078
21	28.1544399971483	17.320521046574395	25.44074062757799	29.08429832869932
22	19.770438041695947	33.6607697556703	27.199120046441994	19.369672156191754
23	29.726440363795614	18.785073380386404	23.174656522757594	28.313829733060388
24	26.458696161406298	28.09485980832493	26.86760976504018	18.578834265228593
25	25.15098740159084	25.43870369804557	22.07216841333374	27.33814048702985
26	25.405603593143695	21.41424017436117	28.004725676515218	25.175430555979915
27	20.212960982614806	29.416827074867346	27.913063847556195	22.457148094961653
28	18.802896513795105	18.86145823785226	28.1814293134529	34.15421593489973
29	18.159736013932598	22.072677645716844	35.51590332732439	24.251683013026167
30	25.523236273641114	17.88118590037378	29.916384042694045	26.679193783291066
31	22.205587297707435	22.298267591432676	21.745241223379878	33.75090388748001
32	27.48327171621498	20.352999887968874	33.670954403332416	18.49277399248373
33	24.282746188395613	19.565217391304348	31.253119048346523	24.898917371953516
34	17.358204242924216	35.650340676464296	24.433988206178007	22.55746687443348
35	25.390326621650523	19.53873730738285	37.5869514294153	17.483984641551327
36	22.991842097222648	19.678266980353815	22.62010245755548	34.709788464868055
37	21.23142574882622	25.14385814822736	29.334331428804223	24.2903846741422
38	20.241987228451833	17.816004155336245	30.99035513866398	30.951653477547946
39	28.840376017191687	19.070752747308706	25.351115728151385	26.737755507348222
40	17.72637925590964	18.893539877987923	41.99283000804587	21.387250858056568
41	23.968040575636284	21.441738723048875	23.652825730493852	30.937394970820986
42	24.55365781620785	17.591941906769733	36.16670231293349	21.68769796408893
43	24.737999938892113	18.120015888050354	33.14899121064907	23.992992962408465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	47.0
4	90.0
5	90.0
6	143.0
7	196.0
8	229.5
9	263.0
10	372.0
11	481.0
12	481.0
13	806.5
14	1132.0
15	2888.0
16	4644.0
17	4606.0
18	4568.0
19	4568.0
20	3704.0
21	2840.0
22	2206.5
23	1573.0
24	1495.0
25	1417.0
26	1417.0
27	1321.0
28	1225.0
29	1177.0
30	1129.0
31	1173.0
32	1217.0
33	1217.0
34	1456.5
35	1696.0
36	1626.0
37	1556.0
38	1720.0
39	1884.0
40	1884.0
41	2594.0
42	3304.0
43	4538.0
44	5772.0
45	8243.0
46	10714.0
47	10714.0
48	13724.0
49	16734.0
50	17487.5
51	18241.0
52	30681.5
53	43122.0
54	43122.0
55	29988.5
56	16855.0
57	23218.0
58	29581.0
59	22690.0
60	15799.0
61	15799.0
62	10279.5
63	4760.0
64	3682.0
65	2604.0
66	2097.5
67	1591.0
68	1591.0
69	1246.5
70	902.0
71	631.5
72	361.0
73	211.0
74	61.0
75	61.0
76	44.5
77	28.0
78	22.5
79	17.0
80	13.0
81	9.0
82	9.0
83	6.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	196374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.039821972358865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72126648540137	14.573212339719108
2	10.713287424084568	4.293847454347317
3	4.474881203466063	2.6902746799474473
4	2.5512667395116	2.0450772505525174
5	1.664422026274998	1.6677360546711886
6	1.12316722994435	1.3504842799963335
7	0.7877417223591594	1.105034271339383
8	0.6733921175005718	1.0795726521840976
9	0.5463370009910299	0.985364661309542
>10	3.8548522348994996	15.964944442747003
>50	0.4929738520570224	6.875655636693248
>100	0.33542550758519046	13.028201289376392
>500	0.027952125632099208	4.028028150366138
>1k	0.02286992097171753	9.727356982085205
>5k	0.005082204660381674	8.090174870400359
>10k+	0.005082204660381674	12.49503498426472
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	13097	6.669416521535437	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	11440	5.825618462729282	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	9904	5.043437522278917	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	5983	3.0467373481214417	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	3675	1.871429007913471	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	3144	1.6010266124843413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3121	1.58931426767291	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2777	1.4141383278845467	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1662	0.846344220721684	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	1360	0.6925560410237608	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	1207	0.6146434864085877	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.5784879872080826	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1020	0.5194170307678205	No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	937	0.4771507429700469	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	930	0.473586116288307	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	754	0.38396121686170265	RNA PCR Primer, Index 26 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	750	0.38192428732927985	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	742	0.37785042826443416	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	726	0.3697027101347429	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	691	0.3518795767260432	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	668	0.3401672319146119	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	644	0.327945654720075	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	554	0.2821147402405614	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	514	0.2617454449163331	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	473	0.24086691720899917	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	452	0.23017303716377932	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	420	0.21387760090439673	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	413	0.21031297422265677	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	412	0.20980374183955106	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	410	0.20878527707333966	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	400	0.20369295324228257	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	397	0.20216525609296548	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	396	0.20165602370985974	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	395	0.20114679132675403	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	379	0.19299907319706275	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	367	0.18688828459979429	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	353	0.17975903123631437	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	342	0.17415747502215162	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	342	0.17415747502215162	No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT	329	0.16753745404177742	RNA PCR Primer, Index 26 (95% over 23bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	311	0.1583712711458747	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	307	0.1563343416134519	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	294	0.14971432063307769	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	290	0.14767739110065486	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	278	0.1415666025033864	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	268	0.13647427867232934	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	265	0.1349465815230122	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	265	0.1349465815230122	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	258	0.13138195484127227	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	255	0.12985425769195513	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	254	0.12934502530884945	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	254	0.12934502530884945	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	250	0.1273080957764266	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	247	0.1257803986271095	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	242	0.12323423671158096	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	241	0.12272500432847526	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	233	0.1186511452636296	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	228	0.11610498334810107	No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	222	0.11304958904946683	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	219	0.11152189190014972	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	218	0.11101265951704402	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	218	0.11101265951704402	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	217	0.1105034271339383	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	215	0.10948496236772688	No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC	209	0.10642956806909264	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	204	0.10388340615356412	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	204	0.10388340615356412	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	201	0.10235570900424701	No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG	199	0.10133724423803558	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	199	0.10133724423803558	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	198	0.10082801185492987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.092323831057064E-4	0.0
10	0.0	0.0	0.0	5.092323831057064E-4	0.0
11	0.0	0.0	0.0	5.092323831057064E-4	0.0
12	0.0	0.0	0.0	5.092323831057064E-4	0.0
13	0.0	0.0	0.0	5.092323831057064E-4	0.0
14	0.0	0.0	0.0	5.092323831057064E-4	0.0
15	0.0	0.0	0.0	5.092323831057064E-4	0.0
16	0.0	0.0	0.0	0.0010184647662114129	0.0
17	0.0	0.0	0.0	0.0010184647662114129	0.0
18	0.0	0.0	0.0	0.0010184647662114129	0.0
19	0.0	0.0	0.0	0.0010184647662114129	0.0
20	0.0	0.0	0.0	0.0015276971493171194	0.0
21	0.0	0.0	0.0	0.0025461619155285322	0.0
22	0.0	0.0	0.0	0.004073859064845651	0.0
23	0.0	0.0	0.0	0.004583091447951358	0.0
24	0.0	0.0	0.0	0.007129253363479891	0.0
25	0.0	0.0	0.0	0.008147718129691303	0.0
26	0.0	0.0	0.0	0.008147718129691303	0.0
27	0.0	0.0	0.0	0.011203112428325543	0.0
28	0.0	0.0	0.0	0.024952386772179615	0.0
29	0.0	0.0	0.0	0.059070956440261946	0.0
30	0.0	0.0	0.0	0.09777261755629564	0.0
31	0.0	0.0	0.0	0.16346359497693178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACA	30	8.276904E-6	37.0	3
TGATACA	25	1.2299007E-4	37.0	2
ATTACGG	20	0.0018382218	37.0	2
GATTACG	20	0.0018382218	37.0	1
TTTACAC	30	8.276904E-6	37.0	2
TTACGGG	20	0.0018382218	37.0	3
CTTATAC	1410	0.0	35.687943	1
CGTCTTC	355	0.0	35.43662	37
TTATACA	1520	0.0	33.105263	2
TTATGAG	35	2.378309E-5	31.714285	3
ATAACAC	35	2.378309E-5	31.714285	4
CTTATAA	35	2.378309E-5	31.714285	1
TATACAC	1600	0.0	31.681252	3
GATACAC	30	3.588713E-4	30.833332	3
ACTCAGA	30	3.588713E-4	30.833332	1
TCGCCGA	30	3.588713E-4	30.833332	1
TCAGAGT	30	3.588713E-4	30.833332	3
GAGGTCG	25	0.005485387	29.6	26
CTTTATG	25	0.005485387	29.6	1
GTCGCTT	25	0.005485387	29.6	29
>>END_MODULE