FastQCFastQC Report
Fri 10 Feb 2017
ERR1631104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612638
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG134112.1890578122806614No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT107181.7494833817033877TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT104061.698556080425961No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC72441.182427469402812No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC41820.6826217113531972RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC33820.5520388875649241No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG17650.28809835498287734No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15380.25104547873295485No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT15150.247291222549042No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13380.2183997727858866No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC13180.2151352021911798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13040.21285000277488497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12690.20713700423414805No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11900.1942419503850561No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA11650.19016123714167257No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC11540.18836572331458382No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA11190.18265272477384686No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10940.17857201153046334No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10630.17351192710866775No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10230.1669827859192541No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT9850.16078010178931115No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT9390.15327158942148544No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT8760.14298819204815894No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC8720.14233527792921757No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8690.14184559234001157No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8460.1380913361560987No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG7900.1289505384909196No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7540.12307431142044732No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA7310.11932005523653447No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT7230.11801422699865174No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG7190.11736131287971036No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG7190.11736131287971036No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA6830.11148508580923809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC13550.033.4501841
TTACGGG352.3865003E-531.7142873
CGTCTTC4750.029.637
GATTACG250.005494364529.61
TTATACA15500.029.5999982
TATACAC16150.029.0959763
GGTATCA3250.029.030771
CCGTCTT15500.026.01935437
GCCGTCT15900.025.36477936
ATTACGG400.001930163423.1252
CTTATAG400.001930163423.1253
GGCGAAC400.001930163423.12529
GTATCAA8250.021.0787871
TGCCGTC19700.020.5659935
CGAACCG450.003823820.55555531
GCGAACC450.003823820.55555530
TCGTAAA450.003823820.55555521
ATGCCGT21000.019.4690534
TATTGTC2100.018.55
ACGCGCA609.2301023E-418.527
Produced by FastQC (version 0.11.5)