##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612638
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.809874673134868	31.0	30.0	33.0	28.0	34.0
2	30.81486946614477	31.0	31.0	34.0	27.0	34.0
3	30.21239785974752	31.0	30.0	34.0	26.0	34.0
4	34.491605156715714	35.0	35.0	37.0	30.0	37.0
5	34.500060394556	35.0	35.0	37.0	30.0	37.0
6	34.73372040258685	35.0	35.0	37.0	32.0	37.0
7	34.770725942563146	35.0	35.0	37.0	32.0	37.0
8	34.82068040180335	35.0	35.0	37.0	32.0	37.0
9	36.52231660458541	38.0	35.0	39.0	32.0	39.0
10	36.26450530329493	38.0	35.0	39.0	32.0	39.0
11	36.35881874777601	38.0	35.0	39.0	32.0	39.0
12	36.27045824777438	38.0	35.0	39.0	32.0	39.0
13	36.37602140252482	38.0	35.0	39.0	32.0	39.0
14	37.31445323339395	39.0	36.0	40.0	32.0	41.0
15	37.34447259229757	39.0	36.0	40.0	32.0	41.0
16	37.32273218442212	39.0	36.0	40.0	32.0	41.0
17	37.26336107130149	39.0	36.0	40.0	32.0	41.0
18	37.32039148730572	39.0	36.0	40.0	32.0	41.0
19	37.351236782569806	39.0	36.0	40.0	32.0	41.0
20	37.343858853025765	39.0	36.0	40.0	32.0	41.0
21	37.28073021915063	39.0	36.0	40.0	32.0	41.0
22	37.119844018816984	39.0	36.0	40.0	31.0	41.0
23	37.136281131761336	39.0	36.0	40.0	31.0	41.0
24	37.03390256562603	39.0	36.0	40.0	31.0	41.0
25	36.95242704500863	39.0	36.0	40.0	31.0	41.0
26	36.830232535363464	39.0	36.0	40.0	31.0	41.0
27	36.71294141075154	39.0	36.0	40.0	30.0	41.0
28	36.7187605078366	38.0	36.0	40.0	30.0	41.0
29	36.58135473150539	38.0	35.0	40.0	30.0	41.0
30	36.49274122728267	38.0	35.0	40.0	30.0	41.0
31	36.48306667232526	38.0	35.0	40.0	30.0	41.0
32	36.258764555904136	38.0	35.0	40.0	30.0	41.0
33	36.240011230122846	38.0	35.0	40.0	30.0	41.0
34	36.22785233694286	38.0	35.0	40.0	30.0	41.0
35	36.157014419608316	38.0	35.0	40.0	29.0	41.0
36	36.20924591683833	38.0	35.0	40.0	30.0	41.0
37	36.19404934071997	38.0	35.0	40.0	30.0	41.0
38	36.125927546120224	38.0	35.0	40.0	29.0	41.0
39	36.019768607236244	38.0	35.0	40.0	29.0	41.0
40	35.889905621264106	38.0	34.0	40.0	29.0	41.0
41	35.8835576637427	38.0	34.0	40.0	29.0	41.0
42	35.835694161968405	38.0	34.0	40.0	29.0	41.0
43	34.995832775635854	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	17.0
11	21.0
12	18.0
13	17.0
14	11.0
15	17.0
16	19.0
17	12.0
18	45.0
19	70.0
20	128.0
21	262.0
22	515.0
23	904.0
24	1619.0
25	2615.0
26	4063.0
27	5755.0
28	8580.0
29	11455.0
30	15655.0
31	20196.0
32	26175.0
33	33422.0
34	43782.0
35	58547.0
36	80979.0
37	112193.0
38	134569.0
39	50961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.01115177315152	24.31648053173326	16.12436708137595	23.548000613739273
2	13.906417819332134	22.74099876272774	40.864262419242685	22.488320998697436
3	15.44500994061746	29.204358854658054	35.123351799921	20.22727940480349
4	12.130328187281886	19.842712988747024	35.66853508923703	32.35842373473405
5	11.741191372392832	40.13381474867703	35.07111867040569	13.053875208524445
6	26.968780911402817	39.51730059186665	18.072336355237514	15.441582141493019
7	22.748833732155042	35.04777699065353	22.405890591181088	19.797498686010336
8	23.73375468057809	36.19102961291987	22.829958311433508	17.24525739506854
9	22.49533982547606	15.734577352367957	18.82220822084167	42.94787460131432
10	13.495081924399074	29.29625651689905	33.97618169294102	23.232479865760858
11	29.663194251744095	23.327152413007354	25.279365628642037	21.73028770660651
12	18.936794648715882	27.699718267557678	28.51977840094803	24.843708682778413
13	28.63550742853039	20.64840901152067	27.26618329258061	23.44990026736833
14	21.03395479875555	23.94823696865033	25.628511453745933	29.389296778848195
15	24.031483518815353	29.52885717177191	22.80988120227606	23.62977810713668
16	24.75687110495921	28.36667004005628	22.481791857508025	24.394666997476484
17	20.298447043768096	32.04926889941532	24.045847629432064	23.606436427384526
18	22.86097173208322	26.175000571299854	25.376160146775096	25.58786754984183
19	24.9267920044137	28.471952441735578	25.460712525178003	21.140543028672724
20	22.43739369742001	25.83173097326643	25.957743398222117	25.77313193109144
21	25.85556233860779	26.404499884107747	25.086755963554335	22.653181813730132
22	19.53665949549326	32.57747642163888	26.525941910230838	21.35992217263702
23	23.67140138221919	27.981287481351142	25.05672191408303	23.29058922234664
24	26.337902643975724	28.53952905304601	25.635040594935344	19.487527708042922
25	21.298385016926797	28.500843891498732	27.021177269447865	23.179593822126606
26	26.432738419751956	28.069757344467693	25.01020178310846	20.487302452671887
27	20.202958353872923	29.107564336524995	27.794554043333907	22.894923266268172
28	21.881600553671174	27.6961272399035	27.532082567519485	22.89018963890585
29	23.655078529245653	26.634652111034573	27.96839242750205	21.74187693221772
30	19.50091244748122	29.253653870638125	28.625387259686796	22.620046422193855
31	25.77819201551324	26.837055487906397	25.641569736124758	21.743182760455603
32	21.531801814448336	27.24839138283946	31.126374792291696	20.093432010420507
33	20.078088528625386	30.19564571574078	28.88655290726334	20.83971284837049
34	21.984597755934175	29.795899046418928	26.44041016064952	21.77909303699738
35	20.916103800286628	26.04996751752258	32.878633059000585	20.155295623190202
36	22.23515354907792	28.625387259686796	24.725367998720287	24.41409119251499
37	22.446697723614925	26.779109359850352	27.913547641510974	22.86064527502375
38	22.18373656221129	25.551141130651377	28.29109523078882	23.974027076348513
39	24.47726063352257	26.179407741602706	26.67921349965232	22.664118125222398
40	20.71076230987957	24.634449707657705	32.431876573114955	22.22291140934777
41	21.79965983174403	27.676213359275785	26.4144568244216	24.109669984558582
42	22.561284151489133	24.853339166032796	30.543485712606795	22.04189096987128
43	22.609599796290794	24.844524825427087	29.41557657213558	23.130298806146534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	13.0
2	16.0
3	52.0
4	88.0
5	88.0
6	114.5
7	141.0
8	150.0
9	159.0
10	257.5
11	356.0
12	356.0
13	533.5
14	711.0
15	1894.0
16	3077.0
17	3304.0
18	3531.0
19	3531.0
20	3896.5
21	4262.0
22	5553.0
23	6844.0
24	8572.5
25	10301.0
26	10301.0
27	12720.5
28	15140.0
29	20257.0
30	25374.0
31	26429.0
32	27484.0
33	27484.0
34	29992.0
35	32500.0
36	33906.5
37	35313.0
38	39203.5
39	43094.0
40	43094.0
41	46219.5
42	49345.0
43	44358.5
44	39372.0
45	40130.5
46	40889.0
47	40889.0
48	42256.0
49	43623.0
50	55448.5
51	67274.0
52	56106.0
53	44938.0
54	44938.0
55	46158.5
56	47379.0
57	38910.0
58	30441.0
59	22052.0
60	13663.0
61	13663.0
62	11822.5
63	9982.0
64	8294.5
65	6607.0
66	5425.5
67	4244.0
68	4244.0
69	3415.5
70	2587.0
71	2185.0
72	1783.0
73	1406.5
74	1030.0
75	1030.0
76	769.5
77	509.0
78	424.0
79	339.0
80	234.0
81	129.0
82	129.0
83	90.5
84	52.0
85	44.0
86	36.0
87	24.5
88	13.0
89	13.0
90	7.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	612638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.17904336237021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11225123285796	41.85739094049669
2	9.135907101100395	8.985903429592044
3	2.252863942674555	3.3238108057893676
4	0.9349263509544012	1.839151342168362
5	0.500812730336678	1.2314745490827255
6	0.32241664095549627	0.9513685179780058
7	0.22731198625808793	0.7825290220281106
8	0.16861829691777364	0.6633989228646564
9	0.13757918087301538	0.6089411251722058
>10	0.9396284732429993	9.061148820872797
>50	0.13218146108881298	4.605432871274899
>100	0.1202253485093982	12.197202356184953
>500	0.008634969782442966	2.916757809217586
>1k	0.00531382755842644	4.1517004565181885
>5k	3.321142224016525E-4	1.183166847909743
>10k+	9.963426672049574E-4	5.640622182849666
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	13411	2.1890578122806614	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	10718	1.7494833817033877	TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	10406	1.698556080425961	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	7244	1.182427469402812	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	4182	0.6826217113531972	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3382	0.5520388875649241	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1765	0.28809835498287734	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1538	0.25104547873295485	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1515	0.247291222549042	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1338	0.2183997727858866	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1318	0.2151352021911798	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	0.21285000277488497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1269	0.20713700423414805	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1190	0.1942419503850561	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1165	0.19016123714167257	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1154	0.18836572331458382	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1119	0.18265272477384686	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1094	0.17857201153046334	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1063	0.17351192710866775	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1023	0.1669827859192541	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	985	0.16078010178931115	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	939	0.15327158942148544	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	876	0.14298819204815894	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	872	0.14233527792921757	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	869	0.14184559234001157	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	846	0.1380913361560987	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	790	0.1289505384909196	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	754	0.12307431142044732	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	731	0.11932005523653447	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	723	0.11801422699865174	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	719	0.11736131287971036	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	719	0.11736131287971036	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	683	0.11148508580923809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.896855892060238E-4	0.0
5	0.0	0.0	0.0	4.896855892060238E-4	0.0
6	0.0	0.0	0.0	4.896855892060238E-4	0.0
7	0.0	0.0	0.0	4.896855892060238E-4	0.0
8	0.0	0.0	0.0	4.896855892060238E-4	0.0
9	0.0	0.0	0.0	4.896855892060238E-4	0.0
10	0.0	0.0	0.0	4.896855892060238E-4	0.0
11	0.0	0.0	0.0	4.896855892060238E-4	0.0
12	0.0	0.0	0.0	4.896855892060238E-4	0.0
13	0.0	0.0	0.0	4.896855892060238E-4	0.0
14	0.0	0.0	0.0	4.896855892060238E-4	0.0
15	0.0	0.0	0.0	4.896855892060238E-4	0.0
16	0.0	0.0	0.0	4.896855892060238E-4	0.0
17	0.0	0.0	0.0	6.52914118941365E-4	0.0
18	0.0	0.0	0.0	6.52914118941365E-4	0.0
19	0.0	0.0	0.0	8.161426486767063E-4	0.0
20	0.0	0.0	0.0	8.161426486767063E-4	0.0
21	0.0	0.0	0.0	9.793711784120477E-4	0.0
22	0.0	0.0	0.0	0.0011425997081473888	0.0
23	0.0	0.0	0.0	0.0011425997081473888	0.0
24	0.0	0.0	0.0	0.0011425997081473888	0.0
25	0.0	0.0	0.0	0.00130582823788273	0.0
26	0.0	0.0	0.0	0.0019587423568240953	0.0
27	0.0	0.0	0.0	0.0022851994162947776	0.0
28	0.0	0.0	0.0	0.005712998540736944	0.0
29	0.0	0.0	0.0	0.01289505384909196	0.0
30	0.0	0.0	0.0	0.0233416797521538	0.0
31	0.0	0.0	0.0	0.056313842758692736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1355	0.0	33.450184	1
TTACGGG	35	2.3865003E-5	31.714287	3
CGTCTTC	475	0.0	29.6	37
GATTACG	25	0.0054943645	29.6	1
TTATACA	1550	0.0	29.599998	2
TATACAC	1615	0.0	29.095976	3
GGTATCA	325	0.0	29.03077	1
CCGTCTT	1550	0.0	26.019354	37
GCCGTCT	1590	0.0	25.364779	36
ATTACGG	40	0.0019301634	23.125	2
CTTATAG	40	0.0019301634	23.125	3
GGCGAAC	40	0.0019301634	23.125	29
GTATCAA	825	0.0	21.078787	1
TGCCGTC	1970	0.0	20.56599	35
CGAACCG	45	0.0038238	20.555555	31
GCGAACC	45	0.0038238	20.555555	30
TCGTAAA	45	0.0038238	20.555555	21
ATGCCGT	2100	0.0	19.46905	34
TATTGTC	210	0.0	18.5	5
ACGCGCA	60	9.2301023E-4	18.5	27
>>END_MODULE