FastQCFastQC Report
Fri 10 Feb 2017
ERR1631103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830746
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT140771.6945010869748396No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT120811.4542351091669414RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG119881.443040351683908No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC61250.7372891353073021No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC48620.585257106263527RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC36180.4355121782109092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.2850450077400312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22330.2687945533291764No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG13870.1669583723544862No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA13000.1564858572897131No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT9780.11772551417641494No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT9560.11507729197612747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9500.11435504955786727No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT8310.10003057492903968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA200.001841642537.033
GTTCGCG200.001841642537.030
GATTACG750.036.9999961
CTTATAC17950.032.259051
TTATACA20000.029.972
TATACAC21100.028.9336513
CGTCTTC6150.028.27642437
TTACGGG1050.026.428573
GGTATCA5000.026.2699991
CCGTCTT18150.024.87052337
GCCGTCT18400.024.6331536
ATTACGG1200.023.1249982
GTATCAA12800.022.4023441
AGGGCGT603.7246864E-521.58333233
AACGGCC705.099115E-621.14285737
CGGATCG450.003824927820.55555737
TGCCGTC22500.020.30888735
TACTAGG555.1414454E-420.1818182
ATGCCGT23550.019.40339734
TTAACGG801.6157916E-518.535
Produced by FastQC (version 0.11.5)