##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631103.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	830746
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291631858594563	31.0	31.0	34.0	28.0	34.0
2	31.293763677465797	31.0	31.0	34.0	28.0	34.0
3	30.751139337414806	31.0	31.0	34.0	26.0	34.0
4	34.943537495215146	37.0	35.0	37.0	32.0	37.0
5	35.12179053525386	37.0	35.0	37.0	32.0	37.0
6	35.322658189145656	37.0	35.0	37.0	33.0	37.0
7	35.32984690868208	37.0	35.0	37.0	33.0	37.0
8	35.35271430738156	37.0	35.0	37.0	33.0	37.0
9	37.101026065728874	39.0	37.0	39.0	33.0	39.0
10	36.915124478480784	39.0	37.0	39.0	33.0	39.0
11	36.99047723371524	39.0	37.0	39.0	33.0	39.0
12	36.925429674052	39.0	37.0	39.0	33.0	39.0
13	37.007025011254946	39.0	37.0	39.0	33.0	39.0
14	38.099355278267964	40.0	37.0	41.0	33.0	41.0
15	38.10136913087755	40.0	37.0	41.0	33.0	41.0
16	38.09596796132632	40.0	37.0	41.0	33.0	41.0
17	38.05365900046464	40.0	37.0	41.0	33.0	41.0
18	38.11290695350925	40.0	37.0	41.0	33.0	41.0
19	38.125811018048836	40.0	37.0	41.0	33.0	41.0
20	38.1333271541482	40.0	37.0	41.0	33.0	41.0
21	38.079532131361454	40.0	37.0	41.0	33.0	41.0
22	38.008867933158875	40.0	37.0	41.0	33.0	41.0
23	38.00461633279004	40.0	37.0	41.0	33.0	41.0
24	37.955973305920224	40.0	37.0	41.0	33.0	41.0
25	37.91443353323398	40.0	37.0	41.0	33.0	41.0
26	37.775877343977584	40.0	37.0	41.0	33.0	41.0
27	37.7149405474116	40.0	37.0	41.0	32.0	41.0
28	37.68428857918064	40.0	37.0	41.0	32.0	41.0
29	37.630052988518756	39.0	37.0	41.0	32.0	41.0
30	37.54146393723232	39.0	37.0	41.0	32.0	41.0
31	37.51448457169821	39.0	37.0	41.0	32.0	41.0
32	37.43039147946544	39.0	36.0	41.0	32.0	41.0
33	37.3931177519964	39.0	36.0	41.0	32.0	41.0
34	37.34102240636729	39.0	36.0	41.0	31.0	41.0
35	37.29177389960349	39.0	36.0	40.0	31.0	41.0
36	37.2701824625096	39.0	36.0	40.0	31.0	41.0
37	37.22646512893231	39.0	36.0	40.0	31.0	41.0
38	37.18122747506458	39.0	36.0	40.0	31.0	41.0
39	37.09813107736902	39.0	36.0	40.0	31.0	41.0
40	36.99612757690076	39.0	36.0	40.0	31.0	41.0
41	36.960605287296	39.0	36.0	40.0	31.0	41.0
42	36.91365953010908	39.0	36.0	40.0	31.0	41.0
43	36.22699477337237	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	8.0
11	8.0
12	8.0
13	6.0
14	4.0
15	3.0
16	6.0
17	23.0
18	21.0
19	54.0
20	100.0
21	198.0
22	366.0
23	683.0
24	1369.0
25	2157.0
26	3504.0
27	5417.0
28	7862.0
29	11119.0
30	15168.0
31	20295.0
32	26124.0
33	33723.0
34	44431.0
35	59523.0
36	82053.0
37	122323.0
38	207839.0
39	186341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.48498337638701	22.718616761320547	15.557944305479655	24.238455556812795
2	13.661094967655577	22.336189400851765	41.079343144595335	22.92337248689732
3	14.733263837562866	28.80290726648097	34.50729825963652	21.956530636319645
4	11.656390762037976	19.59648316091802	38.06085133121315	30.686274745830854
5	12.267407847886117	38.47505735808539	36.09538896365436	13.162145830374145
6	27.034135584161707	40.594718481942735	18.06003278980579	14.31111314408977
7	24.090997729751333	33.72486897318795	23.143415676993932	19.040717620066783
8	24.504963009150813	35.5945138465909	22.470767238120917	17.429755906137377
9	23.676189834197213	15.255324732228623	20.035967672429358	41.0325177611448
10	14.216379013561307	28.95806901267054	34.11933370729441	22.706218266473748
11	30.505954888738557	23.356116069171566	25.521759960324818	20.616169081765065
12	19.841564088180984	26.829740979794064	29.778777147286895	23.549917784738057
13	28.436971107895797	21.048671916566555	27.27548492559699	23.238872049940657
14	21.48839717555065	22.903631194131542	26.870547676425765	28.737423953892044
15	23.54931591605617	29.273087080768367	23.182055646370852	23.995541356804605
16	24.497860958704585	28.019394616405012	23.953530922809136	23.529213502081262
17	20.45101631545623	31.173066135738242	25.551251525737108	22.824666023068424
18	22.94780835538179	27.040756139662424	26.257002742113716	23.75443276284207
19	23.467461775320015	28.916540073620578	26.173944864013787	21.44205328704562
20	24.12831358802811	26.14938862179294	26.23954855033909	23.482749239839855
21	24.75726636059638	26.36052415539768	25.75709061494127	23.12511886906467
22	21.004013260370797	30.550733918670687	26.120739672535287	22.324513148423225
23	23.160990242504926	27.488305691511005	26.508824598613778	22.84187946737029
24	23.944864013790017	28.88909486172669	25.895881532983605	21.270159591499688
25	21.986383322941066	30.26304068873037	25.952938684026165	21.7976373043024
26	23.64417042032101	29.90504919674606	25.965818673818468	20.484961709114458
27	22.405163551795614	29.904086206855045	26.019866481451608	21.67088375989773
28	22.239770038014026	27.406812671983978	27.809823941373175	22.543593348628825
29	21.931372525416915	28.24882695793901	26.436239235578622	23.38356128106545
30	22.604382085499058	28.59983677321347	27.78755480014349	21.008226341143985
31	25.058080327801758	26.506417123886244	26.041533753999417	22.39396879431258
32	21.745515476451285	28.321532694710537	27.317976854537967	22.614974974300207
33	20.67912454589008	29.747720723301708	28.684339136149916	20.888815594658293
34	22.94082667867194	28.63859711632677	26.910391383166456	21.510184821834834
35	21.135461380494156	28.23895631155612	30.413267111728494	20.21231519622123
36	22.575251641295896	28.191529059423697	26.09654455152357	23.136674747756835
37	21.764534526798805	27.24466924908456	28.215122311753532	22.775673912363107
38	22.161406735632795	25.483481112157026	28.5330293495244	23.822082802685777
39	23.72205222775674	26.119897056380655	27.228779915882832	22.92927079997978
40	20.71379218196657	25.781165362216612	31.613754384613348	21.891288071203473
41	21.942687656636327	26.40903477115749	26.96058723123554	24.687690340970644
42	22.506157116615668	25.544871717709146	30.076581771082857	21.872389394592332
43	22.3881908549665	25.00114355049558	29.882057813098108	22.728607781439813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	17.0
2	27.0
3	90.0
4	153.0
5	153.0
6	221.5
7	290.0
8	303.0
9	316.0
10	446.0
11	576.0
12	576.0
13	939.0
14	1302.0
15	2823.5
16	4345.0
17	4898.5
18	5452.0
19	5452.0
20	6195.5
21	6939.0
22	8595.0
23	10251.0
24	13140.5
25	16030.0
26	16030.0
27	19619.0
28	23208.0
29	27171.5
30	31135.0
31	35322.0
32	39509.0
33	39509.0
34	42971.0
35	46433.0
36	49308.0
37	52183.0
38	55035.5
39	57888.0
40	57888.0
41	58046.5
42	58205.0
43	57908.0
44	57611.0
45	57694.0
46	57777.0
47	57777.0
48	57423.5
49	57070.0
50	67972.0
51	78874.0
52	64731.0
53	50588.0
54	50588.0
55	55946.5
56	61305.0
57	52105.5
58	42906.0
59	32941.0
60	22976.0
61	22976.0
62	20213.5
63	17451.0
64	14426.5
65	11402.0
66	9515.0
67	7628.0
68	7628.0
69	6083.5
70	4539.0
71	3789.0
72	3039.0
73	2379.0
74	1719.0
75	1719.0
76	1272.5
77	826.0
78	624.5
79	423.0
80	296.5
81	170.0
82	170.0
83	136.0
84	102.0
85	78.5
86	55.0
87	41.0
88	27.0
89	27.0
90	17.0
91	7.0
92	4.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	830746.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.10497836867027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29944988873092	46.17408855760711
2	10.892417116896828	12.222376534520606
3	2.804658466639711	4.720659078069868
4	1.1580940421784713	2.598993647412362
5	0.6114786436548968	1.715349803758091
6	0.38781281196838435	1.3054937655947638
7	0.27846751706827555	1.0936389815045042
8	0.20322073666111024	0.9121356027549463
9	0.15858130593323194	0.8007480665153515
>10	1.0434850029242517	11.524345618595087
>50	0.10373212637000682	4.007789982948393
>100	0.05496642931076252	5.04318064746719
>500	0.0014971400965673352	0.67677593116125
>1k	0.0012832629399148587	1.8920927096720905
>5k	2.1387715665247646E-4	0.7349738614118185
>10k+	6.416314699574293E-4	4.577357211006569
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	14077	1.6945010869748396	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	12081	1.4542351091669414	RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	11988	1.443040351683908	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	6125	0.7372891353073021	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	4862	0.585257106263527	RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	3618	0.4355121782109092	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2368	0.2850450077400312	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2233	0.2687945533291764	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1387	0.1669583723544862	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1300	0.1564858572897131	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	978	0.11772551417641494	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	956	0.11507729197612747	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.11435504955786727	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	831	0.10003057492903968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2037373637670238E-4	0.0
11	0.0	0.0	0.0	1.2037373637670238E-4	0.0
12	0.0	0.0	0.0	1.2037373637670238E-4	0.0
13	0.0	0.0	0.0	1.2037373637670238E-4	0.0
14	0.0	0.0	0.0	1.2037373637670238E-4	0.0
15	0.0	0.0	0.0	1.2037373637670238E-4	0.0
16	0.0	0.0	0.0	2.4074747275340476E-4	0.0
17	0.0	0.0	0.0	2.4074747275340476E-4	0.0
18	0.0	0.0	0.0	2.4074747275340476E-4	0.0
19	0.0	0.0	0.0	2.4074747275340476E-4	0.0
20	0.0	0.0	0.0	8.426161546369167E-4	0.0
21	0.0	0.0	0.0	0.0010833636273903215	0.0
22	0.0	0.0	0.0	0.001203737363767024	0.0
23	0.0	0.0	0.0	0.0014444848365204287	0.0
24	0.0	0.0	0.0	0.001925979782027238	0.0
25	0.0	0.0	0.0	0.001925979782027238	0.0
26	0.0	0.0	0.0	0.0022871009911573452	0.0
27	0.0	0.0	0.0	0.0032500908821709645	0.0
28	0.0	0.0	0.0	0.008185414073615763	0.0
29	0.0	0.0	0.0	0.020343161447662704	0.0
30	0.0	0.0	0.0	0.035750999703880605	0.0
31	0.0	0.0	0.0	0.07342797918978845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGAA	20	0.0018416425	37.0	33
GTTCGCG	20	0.0018416425	37.0	30
GATTACG	75	0.0	36.999996	1
CTTATAC	1795	0.0	32.25905	1
TTATACA	2000	0.0	29.97	2
TATACAC	2110	0.0	28.933651	3
CGTCTTC	615	0.0	28.276424	37
TTACGGG	105	0.0	26.42857	3
GGTATCA	500	0.0	26.269999	1
CCGTCTT	1815	0.0	24.870523	37
GCCGTCT	1840	0.0	24.63315	36
ATTACGG	120	0.0	23.124998	2
GTATCAA	1280	0.0	22.402344	1
AGGGCGT	60	3.7246864E-5	21.583332	33
AACGGCC	70	5.099115E-6	21.142857	37
CGGATCG	45	0.0038249278	20.555557	37
TGCCGTC	2250	0.0	20.308887	35
TACTAGG	55	5.1414454E-4	20.181818	2
ATGCCGT	2355	0.0	19.403397	34
TTAACGG	80	1.6157916E-5	18.5	35
>>END_MODULE