FastQCFastQC Report
Fri 10 Feb 2017
ERR1631102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324494
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT239177.370552306051883No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG194485.99333115558377No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT158514.8848360832557765TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC97373.0006718151953504No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC70182.1627518536552293RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC47701.4699809549637282TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41441.2770652153814863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39491.21697165432951No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24870.7664240324936671No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG21480.6619536878956159No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA20510.6320609934236072No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT15920.4906099958704937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15690.48352203738744015No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15130.4662643993417444No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13570.4181895505001633No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT11450.35285706361288655No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11140.3433037282661621No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10870.334983081351273No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10780.3322095323796434No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10730.33066867183984916No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10160.3131028616861945No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9750.30046780525988154No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8670.26718521760032543No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7450.22958822042934537No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7030.21664499189507358No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6960.2144877871393616No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6400.19723014909366582No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6380.19661380487774813No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6370.19630563276978927No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6340.19538111644591272No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6050.18644412531510599No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5930.18274606001959975No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5890.18151337158776434No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5890.18151337158776434No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5610.17288455256491644No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5510.1698028314853279No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5460.16826197094553366No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5150.1587086355988092No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5080.15655143084309725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.15192884922371447No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4800.14792261182024938No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT4800.14792261182024938TruSeq Adapter, Index 11 (96% over 25bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4770.14699809549637283No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT4650.14330003020086657TruSeq Adapter, Index 11 (95% over 22bp)
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4400.13559572750189525No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4380.13497938328597756No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4370.1346712111780187No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4320.13313035063822443No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4220.1300486295586359No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3920.12080346631987032No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3870.11926260578007605No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3860.11895443367211721No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3830.11802991734824064No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3800.11710540102436409No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3780.11648905680844639No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3630.11186647518906358No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA3620.11155830308110473No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3610.11125013097314589No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3530.10878475410947507No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3510.10816840989355736No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3510.10816840989355736No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3510.10816840989355736No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3480.1072438935696808No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3430.10570303302988654No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3410.10508668881396882No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3340.10292948405825686No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3290.1013886235184626No Hit
CTGATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT3270.10077227930254488No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3250.10015593508662718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAC200.0018399937.021
GTTTGTG200.0018399937.01
ACAACGA200.0018399937.015
GTTGACA200.0018399937.02
ACGTTTA200.0018399937.026
ATTGTAA200.0018399937.032
GATCACG200.0018399937.022
ATCACGT200.0018399937.023
TATTGTA200.0018399937.031
TTACGGG308.293597E-637.03
GGTTGAC200.0018399937.01
TAAGCCG200.0018399937.036
TCACGTT200.0018399937.024
AGCAACG251.2316738E-436.9999962
AGCCCTA251.2316738E-436.99999614
GCCCTAA251.2316738E-436.99999615
CTTATAC28900.035.463671
CGTCTTC7700.035.07792737
ATTACTT700.034.3571432
TTACTTT603.45608E-1133.9166643
Produced by FastQC (version 0.11.5)