##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324494
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075388142769974	31.0	31.0	34.0	28.0	34.0
2	31.12335821309485	31.0	31.0	34.0	28.0	34.0
3	30.562620572337238	31.0	30.0	34.0	26.0	34.0
4	34.72634624985362	35.0	35.0	37.0	32.0	37.0
5	34.959148705368975	35.0	35.0	37.0	32.0	37.0
6	35.154927363834155	36.0	35.0	37.0	32.0	37.0
7	35.13946636917786	36.0	35.0	37.0	32.0	37.0
8	35.173497815059754	37.0	35.0	37.0	32.0	37.0
9	36.98999981509674	39.0	37.0	39.0	33.0	39.0
10	36.803907005984705	39.0	37.0	39.0	32.0	39.0
11	36.86202210210358	39.0	37.0	39.0	33.0	39.0
12	36.708598618156266	39.0	35.0	39.0	32.0	39.0
13	36.82975956412137	39.0	37.0	39.0	32.0	39.0
14	37.6124951462893	39.0	37.0	40.0	32.0	41.0
15	37.64809210647962	39.0	37.0	40.0	32.0	41.0
16	37.66731896429518	39.0	37.0	40.0	32.0	41.0
17	37.60536712543221	39.0	37.0	40.0	32.0	41.0
18	37.785549193513596	39.0	37.0	41.0	33.0	41.0
19	37.691424186579724	39.0	37.0	41.0	32.0	41.0
20	37.66784285687871	39.0	37.0	41.0	32.0	41.0
21	37.4977657522173	39.0	37.0	41.0	32.0	41.0
22	37.18940874099367	39.0	36.0	40.0	31.0	41.0
23	37.380429222111964	39.0	36.0	40.0	32.0	41.0
24	37.22999808933293	39.0	36.0	40.0	31.0	41.0
25	37.14780242469814	39.0	36.0	40.0	31.0	41.0
26	37.02784334995408	39.0	36.0	40.0	31.0	41.0
27	36.69420389899351	39.0	35.0	40.0	30.0	41.0
28	36.78516397837865	39.0	35.0	40.0	30.0	41.0
29	36.54539683322342	39.0	35.0	40.0	30.0	41.0
30	36.341201994489886	38.0	35.0	40.0	30.0	41.0
31	36.34501408346534	38.0	35.0	40.0	30.0	41.0
32	35.922195787903625	38.0	35.0	40.0	30.0	41.0
33	36.00470578808853	38.0	35.0	40.0	30.0	41.0
34	36.05739705510734	38.0	35.0	40.0	30.0	41.0
35	36.06683020333195	38.0	35.0	40.0	30.0	41.0
36	36.02714071754794	38.0	35.0	40.0	30.0	41.0
37	35.92802948590729	38.0	35.0	40.0	29.0	41.0
38	35.79096994089259	38.0	34.0	40.0	28.0	41.0
39	35.552028696986696	38.0	34.0	40.0	27.0	41.0
40	35.17725443305577	38.0	34.0	40.0	26.0	41.0
41	35.158656246340456	38.0	34.0	40.0	26.0	41.0
42	34.98327549970107	38.0	34.0	40.0	26.0	41.0
43	33.80068352573545	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	22.0
11	17.0
12	22.0
13	28.0
14	21.0
15	11.0
16	19.0
17	11.0
18	29.0
19	44.0
20	100.0
21	178.0
22	339.0
23	569.0
24	1028.0
25	1615.0
26	2499.0
27	3722.0
28	5192.0
29	6913.0
30	8657.0
31	10845.0
32	13324.0
33	16767.0
34	21504.0
35	27614.0
36	36360.0
37	51222.0
38	71094.0
39	44711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70599764556509	22.68485703895912	17.408642378595598	27.200502936880188
2	11.909619284177827	20.33042213415348	44.28155836471553	23.478400216953162
3	13.595628886820712	30.926611894210676	37.001916830511504	18.475842388457107
4	9.18476150560565	21.813346317651483	27.876324369633952	41.125567807108915
5	13.510265212916108	36.700832681035706	31.108433437906402	18.680468668141785
6	25.19584337460785	38.16834825913576	15.02862918882938	21.60717917742701
7	21.399471176662743	33.95779274809396	15.19658298766695	29.44615308757635
8	26.65103206838955	29.646156785641647	24.267320813327828	19.435490332640974
9	19.75013405486696	11.021775441148373	14.303500218802196	54.924590285182475
10	15.203979118257966	27.503127946895784	27.07107065153747	30.22182228330878
11	29.19006206586254	19.320233964264364	23.692888004092524	27.79681596578057
12	18.74302760605743	26.227911764162048	20.645374028487428	34.38368660129309
13	35.6807830036919	15.628948455133223	25.014638175128045	23.67563036604683
14	22.671297466208927	24.939444180786086	21.600707563159872	30.788550789845115
15	29.104082047742025	24.087348302279857	17.725751477685257	29.082818172292864
16	30.42151780926612	21.610877242722516	17.04191757012456	30.925687377886803
17	18.53347057264541	34.5963253557847	17.906956677165063	28.963247394404828
18	30.3669713461574	15.848675168107885	20.54799164237243	33.23636184336228
19	31.448655445092978	24.156070682354684	20.335044715772867	24.060229156779478
20	25.46980837858327	20.74460544725018	20.74799534043773	33.03759083372882
21	32.050515571936614	22.67807725258402	20.710090171158786	24.561317004320575
22	24.833124803540283	34.94363532145432	20.348604288523052	19.874635586482338
23	25.59246087755089	18.593564133697388	23.40382256682712	32.4101524219246
24	30.67637614254809	25.63683766109697	26.14686249976887	17.53992369658607
25	21.557563468045636	32.11892977990348	20.711014687482667	25.612492064568222
26	32.76116045288973	23.29873587801315	21.77883104156009	22.161272627537027
27	28.067082904460484	31.318298643426377	23.756987802547965	16.85763064956517
28	16.87458011550291	25.0389837716568	26.591554851553497	31.494881261286807
29	32.88227209131756	19.85830246476052	24.515399360234706	22.744026083687217
30	24.8929101924843	21.981916460704976	27.96538610883406	25.159787237976666
31	22.900885686638272	24.017085061665238	26.458116328807314	26.623912922889176
32	27.959222666674883	26.768753813629836	26.08399538974526	19.188028129950013
33	16.581816612941996	19.607142196774056	30.839707359766283	32.971333830517665
34	16.39044173389955	29.245224873187176	31.83109703106991	22.53323636184336
35	18.440710768149795	27.11760463983926	36.71778214697344	17.723902445037503
36	22.639555739089168	19.561840896904105	21.68792026971223	36.1106830942945
37	21.098387027186945	25.898167608646077	28.642440230019663	24.36100513414732
38	20.326724068857978	17.772901810202963	31.147571295617176	30.752802825321883
39	28.749375951481383	18.959364425844548	24.906469765234487	27.384789857439586
40	17.721128896065874	18.514055729844003	42.206943733936534	21.557871640153593
41	23.637725196767892	21.715655759428525	23.320307925570273	31.326311118233313
42	25.100926365356525	17.630218124218015	35.7556688259259	21.513186684499562
43	24.69906993657818	17.890623555443245	33.347303802227465	24.063002705751106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	64.0
4	120.0
5	120.0
6	197.0
7	274.0
8	315.0
9	356.0
10	543.5
11	731.0
12	731.0
13	1145.5
14	1560.0
15	3881.0
16	6202.0
17	6286.5
18	6371.0
19	6371.0
20	5235.5
21	4100.0
22	3180.0
23	2260.0
24	2206.0
25	2152.0
26	2152.0
27	2175.0
28	2198.0
29	2100.0
30	2002.0
31	2161.5
32	2321.0
33	2321.0
34	2694.5
35	3068.0
36	3096.0
37	3124.0
38	3496.5
39	3869.0
40	3869.0
41	4997.5
42	6126.0
43	7953.5
44	9781.0
45	13520.5
46	17260.0
47	17260.0
48	22180.0
49	27100.0
50	28743.5
51	30387.0
52	52352.5
53	74318.0
54	74318.0
55	50830.0
56	27342.0
57	37702.5
58	48063.0
59	37218.5
60	26374.0
61	26374.0
62	17132.0
63	7890.0
64	6009.0
65	4128.0
66	3416.0
67	2704.0
68	2704.0
69	2119.5
70	1535.0
71	1054.0
72	573.0
73	336.0
74	99.0
75	99.0
76	78.5
77	58.0
78	38.5
79	19.0
80	14.5
81	10.0
82	10.0
83	9.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	324494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.487534438233066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58390018679731	12.518259197396562
2	11.084481725584181	3.876805118122369
3	4.3791632890424	2.297423064833248
4	2.5763930497303775	1.8021904873433716
5	1.5895393507912452	1.3898562068944262
6	1.205371303704226	1.2647383310631322
7	0.9639445952137596	1.1799910013744477
8	0.7313290804638212	1.0231313984233916
9	0.5533429669051563	0.8708943770917181
>10	4.2364219504458465	15.05174209692629
>50	0.5868255031191626	7.094121925212793
>100	0.44055968702639836	14.756205045393752
>500	0.03172029746590068	3.5723310754590223
>1k	0.028195819969689492	9.890167460723465
>5k	0.0035244774962111865	5.163423668850579
>10k+	0.00528671624431678	18.248719544891433
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	23917	7.370552306051883	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	19448	5.99333115558377	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	15851	4.8848360832557765	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	9737	3.0006718151953504	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	7018	2.1627518536552293	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	4770	1.4699809549637282	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4144	1.2770652153814863	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3949	1.21697165432951	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2487	0.7664240324936671	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	2148	0.6619536878956159	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	2051	0.6320609934236072	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1592	0.4906099958704937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1569	0.48352203738744015	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1513	0.4662643993417444	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1357	0.4181895505001633	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	1145	0.35285706361288655	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1114	0.3433037282661621	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1087	0.334983081351273	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1078	0.3322095323796434	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1073	0.33066867183984916	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1016	0.3131028616861945	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	975	0.30046780525988154	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	867	0.26718521760032543	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	745	0.22958822042934537	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	703	0.21664499189507358	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	696	0.2144877871393616	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	640	0.19723014909366582	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	638	0.19661380487774813	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	637	0.19630563276978927	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	634	0.19538111644591272	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	605	0.18644412531510599	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	593	0.18274606001959975	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	589	0.18151337158776434	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	589	0.18151337158776434	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	561	0.17288455256491644	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	551	0.1698028314853279	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	546	0.16826197094553366	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	515	0.1587086355988092	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	508	0.15655143084309725	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	493	0.15192884922371447	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	480	0.14792261182024938	No Hit
TCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT	480	0.14792261182024938	TruSeq Adapter, Index 11 (96% over 25bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	477	0.14699809549637283	No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT	465	0.14330003020086657	TruSeq Adapter, Index 11 (95% over 22bp)
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	440	0.13559572750189525	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	438	0.13497938328597756	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	437	0.1346712111780187	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	432	0.13313035063822443	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	422	0.1300486295586359	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	392	0.12080346631987032	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	387	0.11926260578007605	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	386	0.11895443367211721	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	383	0.11802991734824064	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	380	0.11710540102436409	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	378	0.11648905680844639	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	363	0.11186647518906358	No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	362	0.11155830308110473	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	361	0.11125013097314589	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	353	0.10878475410947507	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	351	0.10816840989355736	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	351	0.10816840989355736	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	351	0.10816840989355736	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	348	0.1072438935696808	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	343	0.10570303302988654	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	341	0.10508668881396882	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	334	0.10292948405825686	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	329	0.1013886235184626	No Hit
CTGATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	327	0.10077227930254488	No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT	325	0.10015593508662718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.0817210795885285E-4	0.0
4	0.0	0.0	0.0	3.0817210795885285E-4	0.0
5	0.0	0.0	0.0	3.0817210795885285E-4	0.0
6	0.0	0.0	0.0	3.0817210795885285E-4	0.0
7	0.0	0.0	0.0	3.0817210795885285E-4	0.0
8	0.0	0.0	0.0	9.245163238765586E-4	0.0
9	0.0	0.0	0.0	9.245163238765586E-4	0.0
10	0.0	0.0	0.0	0.0012326884318354114	0.0
11	0.0	0.0	0.0	0.0015408605397942642	0.0
12	0.0	0.0	0.0	0.0015408605397942642	0.0
13	0.0	0.0	0.0	0.0015408605397942642	0.0
14	0.0	0.0	0.0	0.0015408605397942642	0.0
15	0.0	0.0	0.0	0.0018490326477531172	0.0
16	0.0	0.0	0.0	0.0018490326477531172	0.0
17	0.0	0.0	0.0	0.0021572047557119702	0.0
18	0.0	0.0	0.0	0.0021572047557119702	0.0
19	0.0	0.0	0.0	0.0021572047557119702	0.0
20	0.0	0.0	0.0	0.0021572047557119702	0.0
21	0.0	0.0	0.0	0.002465376863670823	0.0
22	0.0	0.0	0.0	0.0033898931875473814	0.0
23	0.0	0.0	0.0	0.0036980652955062344	0.0
24	0.0	0.0	0.0	0.005238925835300498	0.0
25	0.0	0.0	0.0	0.00647161426713591	0.0
26	0.0	0.0	0.0	0.0077043026989713215	0.0
27	0.0	0.0	0.0	0.009861507454683291	0.0
28	0.0	0.0	0.0	0.024345596528749375	0.0
29	0.0	0.0	0.0	0.06040173315993516	0.0
30	0.0	0.0	0.0	0.10108045141050374	0.0
31	0.0	0.0	0.0	0.16425573354206857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	0.00183999	37.0	21
GTTTGTG	20	0.00183999	37.0	1
ACAACGA	20	0.00183999	37.0	15
GTTGACA	20	0.00183999	37.0	2
ACGTTTA	20	0.00183999	37.0	26
ATTGTAA	20	0.00183999	37.0	32
GATCACG	20	0.00183999	37.0	22
ATCACGT	20	0.00183999	37.0	23
TATTGTA	20	0.00183999	37.0	31
TTACGGG	30	8.293597E-6	37.0	3
GGTTGAC	20	0.00183999	37.0	1
TAAGCCG	20	0.00183999	37.0	36
TCACGTT	20	0.00183999	37.0	24
AGCAACG	25	1.2316738E-4	36.999996	2
AGCCCTA	25	1.2316738E-4	36.999996	14
GCCCTAA	25	1.2316738E-4	36.999996	15
CTTATAC	2890	0.0	35.46367	1
CGTCTTC	770	0.0	35.077927	37
ATTACTT	70	0.0	34.357143	2
TTACTTT	60	3.45608E-11	33.916664	3
>>END_MODULE