FastQCFastQC Report
Fri 10 Feb 2017
ERR1631101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140578
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG120968.604475807025281No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT111607.938653274338801TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC63994.55192135327007No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT60614.311485438688842No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC49553.5247335998520395RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC33882.4100499366899517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19851.4120274865199391No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15871.1289106403562434No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG13900.9887749149938113No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA10910.7760816059411857No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT10310.733400674358719No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7780.5534294128526512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7500.5335116447808333No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7410.5271095050434634No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6270.4460157350367767No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5260.37416950020629114No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4850.3450041969582723No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4090.2909416836204812No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3770.2681785201098323TruSeq Adapter, Index 11 (95% over 23bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3730.2653331246710011No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3700.2631990780918778No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3290.23403377484385893No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3010.21411600677204112No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2910.20700251817496337No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2590.18423935466431446No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2580.1835280058046067No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2550.18139395922548335No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA2450.1742804706284056No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2390.1700123774701589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.1607648422939578No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2000.1422697719415556No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1970.14013572536243224No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1960.1394243765027245No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT1920.13657898106389338No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1910.13586763220418557No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1910.13586763220418557No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1900.1351562833444778No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1890.13444493448477002No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1880.13373358562506224No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1870.13302223676535446No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1800.12804279474740002No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1800.12804279474740002No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1760.1251973993085689No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1740.12377470158915335No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1740.12377470158915335No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1720.12235200386973781No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1720.12235200386973781No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1720.12235200386973781No Hit
TCGCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1720.12235200386973781TruSeq Adapter, Index 11 (95% over 21bp)
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1710.12164065501003002No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1650.11737256185178335No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1630.11594986413236778No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.11381581755324446No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1580.11239311983382891No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1570.11168177097412113No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1530.10883637553529002No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1510.10741367781587445No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1510.10741367781587445No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1510.10741367781587445No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1470.10456828237704334No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1430.10172288693821223No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1420.10101153807850445No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1420.10101153807850445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC355.584552E-737.01
CTTATAC7850.036.0573231
CGTCTTC6250.032.85637
AGCAACG401.5868773E-632.3752
TTTACAC401.5868773E-632.3752
AGACAGA401.5868773E-632.37515
TTCTGCT352.3735316E-531.71428737
TTATACA8950.031.62572
GATACAC601.2496457E-930.8333343
TGATACA601.2496457E-930.8333342
GGCAGGG303.583566E-430.8333342
CTATACA250.005480148429.5999981
GTCAACG250.005480148429.5999983
GACAGAC453.975443E-628.77777716
GCAACGC453.975443E-628.7777773
ATGTGTA453.975443E-628.7777774
CAGACAC453.975443E-628.77777718
TGTGTAT453.975443E-628.7777775
TATACAC10350.027.5265713
GAGATGT358.830557E-426.4285721
Produced by FastQC (version 0.11.5)