##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140578
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.346014312339058	31.0	30.0	33.0	27.0	34.0
2	30.385465720098452	31.0	30.0	33.0	27.0	34.0
3	29.800303034614235	31.0	30.0	34.0	25.0	34.0
4	33.94375364566291	35.0	33.0	37.0	28.0	37.0
5	33.8607534607122	35.0	33.0	37.0	28.0	37.0
6	34.124863065344506	35.0	35.0	37.0	30.0	37.0
7	34.14490887621107	35.0	35.0	37.0	30.0	37.0
8	34.23558451535802	35.0	35.0	37.0	30.0	37.0
9	35.8931482877833	37.0	35.0	39.0	30.0	39.0
10	35.664392721478464	37.0	35.0	39.0	30.0	39.0
11	35.66797791973139	37.0	35.0	39.0	30.0	39.0
12	35.49389662678371	37.0	35.0	39.0	30.0	39.0
13	35.689332612499825	37.0	35.0	39.0	30.0	39.0
14	36.15438404302238	38.0	35.0	40.0	30.0	41.0
15	36.20644766606439	38.0	35.0	40.0	30.0	41.0
16	36.19826715417775	38.0	35.0	40.0	30.0	41.0
17	36.2485524050705	38.0	35.0	40.0	30.0	41.0
18	36.38522386148615	38.0	35.0	40.0	30.0	41.0
19	36.21158360483148	38.0	35.0	40.0	30.0	41.0
20	36.20631250978105	38.0	35.0	40.0	30.0	41.0
21	36.092304628035684	38.0	34.0	40.0	30.0	41.0
22	35.776913884107046	38.0	34.0	40.0	29.0	41.0
23	35.99505612542503	38.0	34.0	40.0	30.0	41.0
24	35.756419923458864	38.0	34.0	40.0	29.0	41.0
25	35.637176514106045	38.0	34.0	40.0	28.0	41.0
26	35.343289846206375	38.0	34.0	40.0	27.0	41.0
27	34.96102519597661	37.0	33.0	40.0	26.0	40.0
28	35.06471140576762	38.0	33.0	40.0	27.0	41.0
29	34.97560073411202	37.0	33.0	40.0	26.0	40.0
30	34.77487942636828	37.0	33.0	40.0	25.0	40.0
31	34.74286872768143	37.0	33.0	39.0	25.0	41.0
32	34.43770718035539	37.0	33.0	39.0	25.0	40.0
33	34.36491485154149	37.0	33.0	39.0	25.0	40.0
34	34.22412468522813	37.0	33.0	39.0	24.0	40.0
35	34.26477827256043	37.0	33.0	39.0	24.0	40.0
36	34.20829005961104	37.0	33.0	39.0	24.0	40.0
37	34.103586620950644	37.0	33.0	40.0	24.0	41.0
38	33.896143066482665	37.0	32.0	39.0	23.0	41.0
39	33.635896086158574	37.0	32.0	39.0	23.0	40.0
40	33.072066752976994	36.0	31.0	39.0	21.0	40.0
41	33.15473260396364	36.0	31.0	39.0	21.0	40.0
42	32.99539757287769	36.0	31.0	39.0	20.0	40.0
43	31.85450070423537	35.0	29.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	13.0
11	19.0
12	18.0
13	19.0
14	24.0
15	17.0
16	13.0
17	21.0
18	32.0
19	68.0
20	119.0
21	202.0
22	380.0
23	628.0
24	905.0
25	1367.0
26	2018.0
27	2835.0
28	3763.0
29	4861.0
30	6090.0
31	7622.0
32	9178.0
33	10985.0
34	13375.0
35	15577.0
36	17878.0
37	17555.0
38	16963.0
39	8026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.288053607250067	26.97648280669806	20.613467256611987	25.121996329439884
2	11.442046408399607	20.663261676791535	41.661568666505424	26.233123248303432
3	13.539814195677844	32.88210103999204	35.79222922505655	17.78585553927357
4	8.783024370811933	18.953890366913743	23.554183442643943	48.708901819630384
5	13.001323108879056	39.127744028226324	26.765212195364853	21.10572066752977
6	25.752251419140975	33.77199846348646	15.40425955697193	25.071490560400633
7	19.637496621092918	38.078504460157355	13.628732803141316	28.65526611560842
8	28.73778258333452	24.875869623980993	26.795088847472577	19.591258945211912
9	17.168404729047218	11.063608815035069	12.7125154718377	59.05547098408002
10	14.79605628192178	23.381325669734952	27.84361706668184	33.97900098166143
11	27.721265062812105	18.734083569264037	19.306008052469092	34.238643315454766
12	16.331858470030873	29.747897964119563	18.36133676677716	35.558906799072396
13	38.77278094723214	13.71409466630625	20.217245941754754	27.295878444706855
14	23.99024029364481	28.597646857972087	19.365761356684548	28.046351491698555
15	31.01054219010087	25.915861656873762	15.525900211981961	27.547695941043404
16	29.812630710352973	21.606510264764044	15.200102434235799	33.38075659064719
17	16.513963778116064	35.95939620708788	16.15686665054276	31.369773364253295
18	23.969611176713286	13.840714763334233	18.607463472236056	43.58221058771643
19	26.51766279218654	26.289319808220345	18.687134544523325	28.505882855069782
20	26.004780264337235	18.93539529656134	22.8186487217061	32.24117571739533
21	30.31626570302608	19.532928338715873	27.266001792599127	22.884804165658924
22	17.80079386532743	42.78265446940488	19.128882186401857	20.287669478865826
23	25.828365747129705	15.347351648195309	25.617806484656203	33.20647612001878
24	21.425116305538563	30.518288779183088	28.20213689197456	19.85445802330379
25	24.15740727567614	30.607918735506267	20.262060919916344	24.97261306890125
26	28.278251219963295	20.731551167323477	26.532601118240407	24.45759649447282
27	24.597020870975545	35.29143962782227	23.820939265034358	16.290600236167823
28	16.29344563160665	16.2429398625674	38.32676521219536	29.136849293630583
29	16.55308796539999	27.325043747954876	27.389065145328573	28.732803141316566
30	20.195194127103814	21.141999459374865	31.662137745593196	27.000668667928124
31	25.462021084380204	15.49744625759365	33.52729445574699	25.513238202279158
32	21.12492708674188	34.44137774047148	27.26457909487971	17.169116077906928
33	23.517904650798847	19.279688144659904	34.46769764828067	22.734709556260583
34	16.203815675283472	33.49243836162131	29.297614135924537	21.006131827170684
35	19.033561439201012	21.923060507334007	43.646231985090125	15.397146068374854
36	28.91633114712117	18.345687091863592	20.624848838367313	32.11313292264792
37	20.72514902758611	20.54944585923829	31.613054674273357	27.112350438902244
38	20.785613680661267	15.252030900994464	29.056466872483604	34.905888545860655
39	33.052113417462195	18.206974064220574	24.984705999516283	23.75620651880095
40	16.21092916388055	15.78269715033647	45.10520849635078	22.901165189432202
41	24.429853888944216	22.20973409779624	23.321572365519497	30.038839647740044
42	20.114811705956836	15.472549047503877	42.688045071063755	21.724594175475538
43	25.606424902900883	15.934214457454225	32.064761200187796	26.3945994394571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.0
2	8.0
3	25.5
4	43.0
5	43.0
6	72.5
7	102.0
8	130.0
9	158.0
10	220.0
11	282.0
12	282.0
13	489.5
14	697.0
15	1831.5
16	2966.0
17	2892.5
18	2819.0
19	2819.0
20	2364.5
21	1910.0
22	1495.5
23	1081.0
24	998.5
25	916.0
26	916.0
27	903.5
28	891.0
29	840.5
30	790.0
31	791.5
32	793.0
33	793.0
34	981.0
35	1169.0
36	1145.5
37	1122.0
38	1247.5
39	1373.0
40	1373.0
41	1813.0
42	2253.0
43	3010.0
44	3767.0
45	4948.0
46	6129.0
47	6129.0
48	9282.5
49	12436.0
50	20662.0
51	28888.0
52	20483.0
53	12078.0
54	12078.0
55	20463.5
56	28849.0
57	23499.0
58	18149.0
59	11356.0
60	4563.0
61	4563.0
62	3733.0
63	2903.0
64	2209.5
65	1516.0
66	1276.0
67	1036.0
68	1036.0
69	797.5
70	559.0
71	388.0
72	217.0
73	143.0
74	69.0
75	69.0
76	44.0
77	19.0
78	17.0
79	15.0
80	11.5
81	8.0
82	8.0
83	4.0
84	0.0
85	0.5
86	1.0
87	2.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	140578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.929619143820513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11112489917478	17.68128725689653
2	9.29453372215673	4.262402367369005
3	3.8220512502326733	2.629145385479947
4	2.08475522739964	1.9121057348945072
5	1.396041446919402	1.6005349343425002
6	0.9213873549668051	1.2676236679992603
7	0.7104299807656511	1.1402922221115679
8	0.4188124340758206	0.7682567684844002
9	0.35676614754606933	0.7362460697975501
>10	3.248123099832475	14.515784831196916
>50	0.37848234783148227	5.815988276970792
>100	0.2078550598746665	8.855581954502128
>500	0.015511571632437798	2.434235797920016
>1k	0.021716200285412917	10.97397885871189
>5k	0.00620462865297512	8.863406791958912
>10k+	0.00620462865297512	16.543129081364082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	12096	8.604475807025281	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	11160	7.938653274338801	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	6399	4.55192135327007	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	6061	4.311485438688842	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	4955	3.5247335998520395	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3388	2.4100499366899517	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1985	1.4120274865199391	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1587	1.1289106403562434	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1390	0.9887749149938113	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1091	0.7760816059411857	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1031	0.733400674358719	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	778	0.5534294128526512	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	750	0.5335116447808333	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	741	0.5271095050434634	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	627	0.4460157350367767	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	526	0.37416950020629114	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	485	0.3450041969582723	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	409	0.2909416836204812	No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	377	0.2681785201098323	TruSeq Adapter, Index 11 (95% over 23bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT	373	0.2653331246710011	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	370	0.2631990780918778	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	329	0.23403377484385893	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	301	0.21411600677204112	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	291	0.20700251817496337	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	259	0.18423935466431446	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	258	0.1835280058046067	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	255	0.18139395922548335	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	245	0.1742804706284056	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	239	0.1700123774701589	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.1607648422939578	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	200	0.1422697719415556	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	197	0.14013572536243224	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	196	0.1394243765027245	No Hit
CTGATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	192	0.13657898106389338	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	191	0.13586763220418557	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	191	0.13586763220418557	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	190	0.1351562833444778	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	189	0.13444493448477002	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	188	0.13373358562506224	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	187	0.13302223676535446	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	180	0.12804279474740002	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	180	0.12804279474740002	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	176	0.1251973993085689	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	174	0.12377470158915335	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	174	0.12377470158915335	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	172	0.12235200386973781	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	172	0.12235200386973781	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	172	0.12235200386973781	No Hit
TCGCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	172	0.12235200386973781	TruSeq Adapter, Index 11 (95% over 21bp)
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	171	0.12164065501003002	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	165	0.11737256185178335	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	163	0.11594986413236778	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	160	0.11381581755324446	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	158	0.11239311983382891	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	157	0.11168177097412113	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	153	0.10883637553529002	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	151	0.10741367781587445	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	151	0.10741367781587445	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	151	0.10741367781587445	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	147	0.10456828237704334	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	143	0.10172288693821223	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	142	0.10101153807850445	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	142	0.10101153807850445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.113488597077779E-4	0.0
2	0.0	0.0	0.0	7.113488597077779E-4	0.0
3	0.0	0.0	0.0	7.113488597077779E-4	0.0
4	0.0	0.0	0.0	7.113488597077779E-4	0.0
5	0.0	0.0	0.0	7.113488597077779E-4	0.0
6	0.0	0.0	0.0	7.113488597077779E-4	0.0
7	0.0	0.0	0.0	7.113488597077779E-4	0.0
8	0.0	0.0	0.0	7.113488597077779E-4	0.0
9	0.0	0.0	0.0	7.113488597077779E-4	0.0
10	0.0	0.0	0.0	7.113488597077779E-4	0.0
11	0.0	0.0	0.0	7.113488597077779E-4	0.0
12	0.0	0.0	0.0	7.113488597077779E-4	0.0
13	0.0	0.0	0.0	7.113488597077779E-4	0.0
14	0.0	0.0	0.0	7.113488597077779E-4	0.0
15	0.0	0.0	0.0	7.113488597077779E-4	0.0
16	0.0	0.0	0.0	7.113488597077779E-4	0.0
17	0.0	0.0	0.0	7.113488597077779E-4	0.0
18	0.0	0.0	0.0	7.113488597077779E-4	0.0
19	0.0	0.0	0.0	7.113488597077779E-4	0.0
20	0.0	0.0	0.0	7.113488597077779E-4	0.0
21	0.0	0.0	0.0	7.113488597077779E-4	0.0
22	0.0	0.0	0.0	7.113488597077779E-4	0.0
23	0.0	0.0	0.0	7.113488597077779E-4	0.0
24	0.0	0.0	0.0	0.0021340465791233336	0.0
25	0.0	0.0	0.0	0.0021340465791233336	0.0
26	0.0	0.0	0.0	0.0028453954388311118	0.0
27	0.0	0.0	0.0	0.004268093158246667	0.0
28	0.0	0.0	0.0	0.011381581755324447	0.0
29	0.0	0.0	0.0	0.029165303248018894	0.0
30	0.0	0.0	0.0	0.046237675881005566	0.0
31	0.0	0.0	0.0	0.07682567684844001	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCAAC	35	5.584552E-7	37.0	1
CTTATAC	785	0.0	36.057323	1
CGTCTTC	625	0.0	32.856	37
AGCAACG	40	1.5868773E-6	32.375	2
TTTACAC	40	1.5868773E-6	32.375	2
AGACAGA	40	1.5868773E-6	32.375	15
TTCTGCT	35	2.3735316E-5	31.714287	37
TTATACA	895	0.0	31.6257	2
GATACAC	60	1.2496457E-9	30.833334	3
TGATACA	60	1.2496457E-9	30.833334	2
GGCAGGG	30	3.583566E-4	30.833334	2
CTATACA	25	0.0054801484	29.599998	1
GTCAACG	25	0.0054801484	29.599998	3
GACAGAC	45	3.975443E-6	28.777777	16
GCAACGC	45	3.975443E-6	28.777777	3
ATGTGTA	45	3.975443E-6	28.777777	4
CAGACAC	45	3.975443E-6	28.777777	18
TGTGTAT	45	3.975443E-6	28.777777	5
TATACAC	1035	0.0	27.526571	3
GAGATGT	35	8.830557E-4	26.428572	1
>>END_MODULE