FastQCFastQC Report
Fri 10 Feb 2017
ERR1631100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357666
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT83902.345763925002656No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG78592.1973013929196514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70881.981737151420599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64891.814262468336381No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT52001.4538703706810265No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC50501.4119318022959968No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA33530.9374667986333619No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC24860.6950618733678907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23350.652843714526961No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23270.6506069908797594No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19430.5432442558140835No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19400.542405484446383No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC15870.44371005351361326Illumina PCR Primer Index 7 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT15310.42805298798320224No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14170.3961796760105797No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13830.386673600509973No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13790.3855552386863722No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13050.3648655449497576No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG12270.3430574893895422No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11760.3287983761386321No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10590.29608629279830906No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9850.2753965990616944No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9470.2647721617374869No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9340.26113748581078433No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG9300.2600191239871836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.24911509620707586No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8900.2488355057511757No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8630.24128656344187036No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8580.23988861116236937No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8540.23877024933876856No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8150.22786622155866088No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8150.22786622155866088No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8070.2256294979114593No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA7990.22339277426425774No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7640.21360710830775081No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7460.20857448010154722No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7240.20242349007174293No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7200.20130512824814212No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7030.19655209049783876No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6950.1943153668506372No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT6920.19347659548293658No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6870.1920786432034356No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6630.18536847226183087No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6390.17865830132022614No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6380.17837871086432594No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.17558280630532397No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6260.17502362539352356No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6170.1725073112904218No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6110.1708297685550206No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6070.16971140673141982No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6050.16915222581961942No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT5840.16328082624571527RNA PCR Primer, Index 41 (95% over 21bp)
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5560.15545229348050976No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5430.15181761755380718No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA5370.150140074818406No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5350.1495808939066056No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5290.14790335117120443No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5250.14678498934760362No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5240.14650539889170344No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG5110.14287072296500086No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5050.14119318022959967No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5050.14119318022959967No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5010.1400748184059989No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4970.1389564565823981No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4910.13727891384699692No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4820.13476259974389515No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4710.13168710472899298No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4680.13084833336129237No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4660.13028915244949196No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4530.1266544765227894No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4420.12357898150788724No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4360.12190143877248608No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4350.12162184831658587No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4300.12022389603708489No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4300.12022389603708489No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4290.11994430558118468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4270.11938512466938428No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4220.1179871723898833No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4020.11239536327187936No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3970.11099741099237838No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3950.11043823008057797No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3940.11015863962467777No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3900.10904027780107699No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3840.1073627350656758No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT3830.1070831446097756No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3770.10540560187437442No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3750.10484642096257402No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3730.10428724005077361No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3710.10372805913897323No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3710.10372805913897323No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG3700.10344846868307303No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3690.10316887822717283No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG251.2319256E-437.02
TAGCAAC251.2319256E-437.01
ACTCAGA750.037.01
CATCAGA200.001840241537.01
ATTACGG1100.035.318182
TTACGGG1100.035.318183
GATTACG1100.035.318181
TCAGAGT800.034.68753
CTTATAC10800.034.087961
CGTCTTC2550.032.6470637
GGTCGCT401.5959904E-632.37528
GAGGTCG401.5959904E-632.37526
GCGAGGT401.5959904E-632.37524
TTATACA11500.031.8521752
GTATACC352.383743E-531.7142871
CTCAGAG850.030.4705892
TATACAC12400.029.838713
AGCTTCC502.7119313E-729.610
GTCTCCC250.00549134429.619
AACATTC250.00549134429.631
Produced by FastQC (version 0.11.5)