FastQCFastQC Report
Fri 10 Feb 2017
ERR1631099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791470
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT121101.5300643107129772No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG119871.5145236079699798No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT108471.370487826449518TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC63510.8024309196810997No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC42500.5369755012824239RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC30800.38914930445879187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17350.21921235170000125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17130.2164327138110099No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG13840.17486449265291165No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA11610.14668907223268096No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT9550.12066155381757994No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT8900.11244898732737817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15950.030.968651
TTATACA17750.028.036622
CGTCTTC5900.027.5932237
TATACAC18800.027.3563843
ATAATCG451.3227036E-424.66666615
CCGTCTT16950.023.90265737
GCCGTCT17000.023.83235236
GGTATCA3900.023.7179491
GTCGCAA400.001930658423.12500218
GTATCAA8750.021.9885711
ATACCGC450.003824771420.5555556
TGCCGTC19800.020.55555535
ATGCCGT21000.019.29285634
CGAAATT801.6156337E-518.520
CGGCGTC500.00703299618.49999824
CGCTATC500.00703299618.49999812
CGTCGCA500.00703299618.49999817
ATCGCTA500.00703299618.49999810
TGCACGC500.00703299618.49999837
CGCCATT500.00703299618.49999833