##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791470
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081909611229737	31.0	31.0	34.0	28.0	34.0
2	31.08017233754912	31.0	31.0	34.0	28.0	34.0
3	30.515442151945116	31.0	30.0	34.0	26.0	34.0
4	34.74117401796657	37.0	35.0	37.0	32.0	37.0
5	34.8684334213552	35.0	35.0	37.0	32.0	37.0
6	35.07473688200437	36.0	35.0	37.0	32.0	37.0
7	35.0942145627756	36.0	35.0	37.0	32.0	37.0
8	35.130846399737194	37.0	35.0	37.0	32.0	37.0
9	36.86584330423137	39.0	37.0	39.0	33.0	39.0
10	36.63138842912555	38.0	35.0	39.0	32.0	39.0
11	36.713737728530454	38.0	35.0	39.0	32.0	39.0
12	36.630368807409	38.0	35.0	39.0	32.0	39.0
13	36.731973416554005	38.0	37.0	39.0	32.0	39.0
14	37.77498325899908	39.0	37.0	40.0	33.0	41.0
15	37.7839779145135	39.0	37.0	40.0	33.0	41.0
16	37.764812311268905	39.0	37.0	40.0	33.0	41.0
17	37.70128874120308	39.0	37.0	40.0	32.0	41.0
18	37.76196191896092	39.0	37.0	40.0	33.0	41.0
19	37.78280035882598	39.0	37.0	41.0	32.0	41.0
20	37.80356804427205	39.0	37.0	41.0	33.0	41.0
21	37.76813271507448	39.0	37.0	40.0	33.0	41.0
22	37.68327163379534	39.0	37.0	40.0	32.0	41.0
23	37.67553160574627	39.0	37.0	40.0	32.0	41.0
24	37.60606087406977	39.0	37.0	40.0	32.0	41.0
25	37.59035212958167	39.0	37.0	40.0	32.0	41.0
26	37.42919125172148	39.0	36.0	40.0	32.0	41.0
27	37.34745220918039	39.0	36.0	40.0	32.0	41.0
28	37.339223217557205	39.0	36.0	40.0	32.0	41.0
29	37.26932669589498	39.0	36.0	40.0	31.0	41.0
30	37.20700847789556	39.0	36.0	40.0	31.0	41.0
31	37.18288248449088	39.0	36.0	40.0	31.0	41.0
32	37.08788962310637	39.0	36.0	40.0	31.0	41.0
33	37.03560084399914	39.0	36.0	40.0	31.0	41.0
34	36.94568966606441	39.0	36.0	40.0	31.0	41.0
35	36.92464022641414	39.0	36.0	40.0	31.0	41.0
36	36.91629373191656	39.0	36.0	40.0	31.0	41.0
37	36.85151427091362	39.0	36.0	40.0	30.0	41.0
38	36.81550785247703	39.0	36.0	40.0	30.0	41.0
39	36.760572100016425	39.0	35.0	40.0	30.0	41.0
40	36.66425764716288	39.0	35.0	40.0	30.0	41.0
41	36.636107496178	39.0	35.0	40.0	30.0	41.0
42	36.5757261803985	39.0	35.0	40.0	30.0	41.0
43	35.852009551846564	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	16.0
11	21.0
12	17.0
13	22.0
14	14.0
15	19.0
16	19.0
17	12.0
18	22.0
19	55.0
20	124.0
21	240.0
22	400.0
23	854.0
24	1430.0
25	2418.0
26	3798.0
27	5931.0
28	8388.0
29	11968.0
30	16364.0
31	21589.0
32	27908.0
33	36393.0
34	47629.0
35	63954.0
36	88661.0
37	130235.0
38	200230.0
39	122728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.55720368428367	22.905226982703073	15.299632329715593	24.23793700329766
2	14.355439877695932	22.089403262284105	40.086674163265826	23.46848269675414
3	15.47980340379294	28.583395454028583	33.4787168180727	22.45808432410578
4	11.988957256750098	19.536179514068756	37.259150694277736	31.215712534903407
5	12.66428291659823	38.44441355957901	35.45642917609006	13.4348743477327
6	28.141433029678954	39.921664750401156	17.65057424791843	14.286327972001466
7	24.591456403906655	33.468988085461234	22.74653492867702	19.193020581955096
8	24.651597660050285	35.679684637446776	21.942840537228196	17.725877165274742
9	23.735580628450858	15.057677486196571	19.523418449214752	41.68332343613782
10	14.047026419194664	28.980630977800804	33.92914450326607	23.04319809973846
11	31.59247981603851	22.900425789985725	25.06146790149974	20.445626492476027
12	20.31978470441078	26.478072447471163	29.622727330157804	23.57941551796025
13	28.93173462038991	20.84728416743528	26.15437098058044	24.066610231594375
14	21.52981161635943	22.333885049338573	26.276927742049605	29.85937559225239
15	23.76110275815887	29.322652785323513	22.4570735466916	24.45917090982602
16	24.83795974578948	27.690120914248173	23.509798223558693	23.962121116403654
17	20.87078474231493	30.706912454041213	25.0954552920515	23.32684751159235
18	23.314591835445437	26.598986695642285	25.839892857594098	24.24652861131818
19	23.02083464944976	29.11430629082593	25.90230836291963	21.96255069680468
20	22.01460573363488	26.31938039344511	26.90575764084552	24.760256232074497
21	23.638040607982614	26.22790503746194	26.103705762694734	24.030348591860715
22	21.019748063729516	29.60238543469746	27.52119473890356	21.856671762669464
23	22.705977484933097	26.285772044423666	25.895738309727466	25.112512160915763
24	23.134294414191313	29.883255208662362	25.681832539451904	21.300617837694418
25	23.472020417703767	27.239693229054794	26.53972986973606	22.748556483505375
26	24.34318420154902	28.29898795911405	25.658711006102568	21.69911683323436
27	21.48900147826197	28.796669488420278	28.356854966075783	21.35747406724197
28	21.408391979481216	25.996689703968567	27.06318622310385	25.531732093446376
29	21.762290421620527	28.259946681491403	27.81065612088898	22.16710677599909
30	22.257318660214537	25.33854726016147	29.17596371309083	23.22817036653316
31	22.97711852628653	26.90601033519906	25.72201094166551	24.394860196848903
32	23.761481799689186	26.497656259870872	28.359508256788	21.381353683651938
33	22.70446131881183	26.105980011876635	28.215725169621088	22.97383349969045
34	21.919087267994996	29.58229623359066	26.476556281349893	22.02206021706445
35	23.686305229509646	25.594147598771904	29.889193525970665	20.830353645747785
36	24.854637573123426	25.99100408101381	25.314541296574728	23.839817049288033
37	22.06312304951546	26.80872300908436	27.888359634603965	23.239794306796217
38	22.205642664914652	25.658584658925797	27.960251178187423	24.175521497972127
39	24.10413534309576	25.964597521068395	26.71017221120194	23.22109492463391
40	21.307440585240123	25.249219806183433	31.31274716666456	22.130592441911887
41	22.466170543419206	26.426017410640963	26.41186652684246	24.695945519097375
42	22.55625608045788	25.317826323170813	29.79759182281072	22.32832577356059
43	23.172577608753333	24.9164213425651	29.08802607805729	22.822974970624283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	8.0
2	11.0
3	61.5
4	112.0
5	112.0
6	144.0
7	176.0
8	181.0
9	186.0
10	287.0
11	388.0
12	388.0
13	582.5
14	777.0
15	1892.0
16	3007.0
17	3550.0
18	4093.0
19	4093.0
20	4523.0
21	4953.0
22	6229.5
23	7506.0
24	9827.0
25	12148.0
26	12148.0
27	15695.0
28	19242.0
29	22798.5
30	26355.0
31	30007.5
32	33660.0
33	33660.0
34	38109.0
35	42558.0
36	44723.5
37	46889.0
38	50194.0
39	53499.0
40	53499.0
41	55781.5
42	58064.0
43	58865.0
44	59666.0
45	59409.0
46	59152.0
47	59152.0
48	58412.0
49	57672.0
50	67498.0
51	77324.0
52	62575.5
53	47827.0
54	47827.0
55	53792.5
56	59758.0
57	50176.5
58	40595.0
59	32703.5
60	24812.0
61	24812.0
62	21639.0
63	18466.0
64	15473.0
65	12480.0
66	10280.5
67	8081.0
68	8081.0
69	6559.5
70	5038.0
71	4141.0
72	3244.0
73	2495.0
74	1746.0
75	1746.0
76	1360.5
77	975.0
78	751.0
79	527.0
80	389.0
81	251.0
82	251.0
83	196.5
84	142.0
85	100.0
86	58.0
87	39.0
88	20.0
89	20.0
90	13.0
91	6.0
92	3.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	791470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.12788596352888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.60443739361438	50.26958077648235
2	9.874930199064833	11.875173538143557
3	2.6528735770393386	4.785350397476409
4	1.1398866748906369	2.741559039966813
5	0.613868072211948	1.8455294721305657
6	0.3786772781261512	1.366143851768924
7	0.27633082744609594	1.1630631936620504
8	0.19631464848745722	0.944318783777927
9	0.15275606134109582	0.8266409132899709
>10	1.0056954535160523	11.498673713701486
>50	0.07691188645117138	3.1511261684740686
>100	0.024375997126451984	2.2440022954300898
>500	8.405516241049273E-4	0.38777269365941086
>1k	0.001260827436157391	1.6833807747228189
>5k	2.1013790602623182E-4	0.8024582526656627
>10k+	6.304137180786955E-4	4.415226134647916
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	12110	1.5300643107129772	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	11987	1.5145236079699798	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	10847	1.370487826449518	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	6351	0.8024309196810997	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	4250	0.5369755012824239	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	3080	0.38914930445879187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1735	0.21921235170000125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1713	0.2164327138110099	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1384	0.17486449265291165	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1161	0.14668907223268096	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	955	0.12066155381757994	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	890	0.11244898732737817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.26347176772335E-4	0.0
4	0.0	0.0	0.0	1.26347176772335E-4	0.0
5	0.0	0.0	0.0	1.26347176772335E-4	0.0
6	0.0	0.0	0.0	1.26347176772335E-4	0.0
7	0.0	0.0	0.0	1.26347176772335E-4	0.0
8	0.0	0.0	0.0	1.26347176772335E-4	0.0
9	0.0	0.0	0.0	1.26347176772335E-4	0.0
10	0.0	0.0	0.0	2.5269435354467E-4	0.0
11	0.0	0.0	0.0	2.5269435354467E-4	0.0
12	0.0	0.0	0.0	2.5269435354467E-4	0.0
13	0.0	0.0	0.0	2.5269435354467E-4	0.0
14	0.0	0.0	0.0	2.5269435354467E-4	0.0
15	0.0	0.0	0.0	2.5269435354467E-4	0.0
16	0.0	0.0	0.0	2.5269435354467E-4	0.0
17	0.0	0.0	0.0	3.790415303170051E-4	0.0
18	0.0	0.0	0.0	5.0538870708934E-4	0.0
19	0.0	0.0	0.0	5.0538870708934E-4	0.0
20	0.0	0.0	0.0	5.0538870708934E-4	0.0
21	0.0	0.0	0.0	0.0012634717677233501	2.5269435354467E-4
22	0.0	0.0	0.0	0.0016425132980403554	2.5269435354467E-4
23	0.0	0.0	0.0	0.00202155482835736	2.5269435354467E-4
24	0.0	0.0	0.0	0.0026532907122190355	2.5269435354467E-4
25	0.0	0.0	0.0	0.0026532907122190355	2.5269435354467E-4
26	0.0	0.0	0.0	0.0027796378889913707	2.5269435354467E-4
27	0.0	0.0	0.0	0.0030323322425360407	2.5269435354467E-4
28	0.0	0.0	0.0	0.005938317308299746	2.5269435354467E-4
29	0.0	0.0	0.0	0.011371245909510152	2.5269435354467E-4
30	0.0	0.0	0.0	0.022742491819020303	2.5269435354467E-4
31	0.0	0.0	0.0	0.057993354138501776	2.5269435354467E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1595	0.0	30.96865	1
TTATACA	1775	0.0	28.03662	2
CGTCTTC	590	0.0	27.59322	37
TATACAC	1880	0.0	27.356384	3
ATAATCG	45	1.3227036E-4	24.666666	15
CCGTCTT	1695	0.0	23.902657	37
GCCGTCT	1700	0.0	23.832352	36
GGTATCA	390	0.0	23.717949	1
GTCGCAA	40	0.0019306584	23.125002	18
GTATCAA	875	0.0	21.988571	1
ATACCGC	45	0.0038247714	20.555555	6
TGCCGTC	1980	0.0	20.555555	35
ATGCCGT	2100	0.0	19.292856	34
CGAAATT	80	1.6156337E-5	18.5	20
CGGCGTC	50	0.007032996	18.499998	24
CGCTATC	50	0.007032996	18.499998	12
CGTCGCA	50	0.007032996	18.499998	17
ATCGCTA	50	0.007032996	18.499998	10
TGCACGC	50	0.007032996	18.499998	37
CGCCATT	50	0.007032996	18.499998	33
>>END_MODULE