FastQCFastQC Report
Fri 10 Feb 2017
ERR1631098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314131
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT120280.9152816576125211No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG107480.8178788872646638No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT80740.6143984123348433RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC59760.45474918406155856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33750.25682371087813927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.2445722686703228No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC29130.22166739845570954RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC28800.2191562332826788RNA PCR Primer, Index 29 (95% over 24bp)
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG15490.11787257130377413No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14980.11399167967272669No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC14020.1066864718966374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17400.028.7068981
TATACAC18200.028.1565953
TTATACA17850.028.0868362
CGTCTTC4500.027.13333337
GGTATCA7000.026.1642861
GCGCTAT509.092386E-625.89999819
GTATCAA20400.025.5735281
GATTACG1250.025.1600021
CCGTCTT13500.025.07777837
GCCGTCT13550.024.71217536
TTACGGG1300.024.1923083
ACGTTAA555.143632E-420.1818181
ACGCTAA555.143632E-420.1818186
TGCCGTC16800.019.9315535
GTTCGCA759.267433E-619.73333235
TTAACGG1153.0522642E-919.30434835
ATGCCGT17850.018.75910434
TTAGCGA1351.1532393E-917.81481627
TATGCCG19300.017.73316233
CTACCGC1156.4070264E-817.69565216
Produced by FastQC (version 0.11.5)