FastQCFastQC Report
Fri 10 Feb 2017
ERR1631097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140127
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT1777712.686348812148978No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG1671911.93131944593119No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1567311.184853739821733TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC86336.16083980960129No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC68024.8541680047385585RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC47593.396204871295325No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG19421.385885660864787No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA18101.2916853996731537No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT14971.068316598514205No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11380.8121204336066569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.680097340269898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8350.5958880158713167No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT6030.43032392044359763TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT5160.36823738465820294TruSeq Adapter, Index 8 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3720.26547346335823935No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA3330.23764156800616582No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3240.2312188229249181No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT2990.21337786436589667No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2750.19625054414923607No Hit
TCGCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT2160.1541458819499454TruSeq Adapter, Index 8 (95% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2130.15200496692286283No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2100.14986405189578025No Hit
CTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA1990.14201403012981081No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1890.13487764670620225No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1680.1198912415166242No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG1530.10918666638121133No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1410.10062300627288104No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1410.10062300627288104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCAA200.001836425237.028
GGCACAA200.001836425237.036
CTATACA200.001836425237.01
GAAGCCA200.001836425237.020
AGGGCAC200.001836425237.034
AAGGGCA200.001836425237.033
TGATACA251.2281003E-437.02
CGGAAGC200.001836425237.018
TCGCCGA200.001836425237.01
TTTACAC403.792411E-837.02
AAGCCAC200.001836425237.021
CTCAAGG200.001836425237.030
CTTATAC19500.036.6205141
CGTCTTC8750.033.40571637
TTATACA21800.032.5871542
CAGACAC352.3734774E-531.71428718
GATACAC303.5835087E-430.8333323
GGAAGCC303.5835087E-430.83333219
TATACAC24000.029.9083333
ACGCCTC250.00548008829.626
Produced by FastQC (version 0.11.5)