##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631097.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	140127
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.858806653963903	31.0	30.0	34.0	27.0	34.0
2	31.063292584583984	31.0	31.0	34.0	28.0	34.0
3	30.570446808966153	31.0	30.0	34.0	26.0	34.0
4	34.61364333782925	35.0	35.0	37.0	30.0	37.0
5	34.75940396925646	35.0	35.0	37.0	32.0	37.0
6	34.937321144390445	35.0	35.0	37.0	32.0	37.0
7	34.87432828791025	35.0	35.0	37.0	32.0	37.0
8	34.965502722530275	35.0	35.0	37.0	32.0	37.0
9	36.8102007464657	39.0	37.0	39.0	32.0	39.0
10	36.62337022843563	38.0	35.0	39.0	32.0	39.0
11	36.5777758747422	38.0	35.0	39.0	32.0	39.0
12	36.442512863331125	38.0	35.0	39.0	32.0	39.0
13	36.61957367245427	39.0	35.0	39.0	32.0	39.0
14	37.243864494351556	39.0	36.0	40.0	31.0	41.0
15	37.21606114453317	39.0	36.0	40.0	31.0	41.0
16	37.20579902517002	39.0	36.0	40.0	31.0	41.0
17	37.00962698123845	39.0	36.0	40.0	31.0	41.0
18	37.22370421117986	39.0	36.0	40.0	31.0	41.0
19	37.22322607349048	39.0	36.0	40.0	31.0	41.0
20	37.329515368201704	39.0	36.0	40.0	31.0	41.0
21	37.34257494986691	39.0	36.0	40.0	31.0	41.0
22	37.00254055249881	39.0	36.0	40.0	31.0	41.0
23	37.109208075531484	39.0	36.0	40.0	31.0	41.0
24	36.94449320973117	39.0	36.0	40.0	31.0	41.0
25	36.66149278868455	38.0	35.0	40.0	30.0	41.0
26	36.489056356019894	38.0	35.0	40.0	30.0	41.0
27	36.3740107188479	38.0	35.0	40.0	30.0	41.0
28	36.461117414916465	38.0	35.0	40.0	30.0	41.0
29	36.56515874884926	38.0	35.0	40.0	30.0	41.0
30	36.42667722851414	38.0	35.0	40.0	30.0	41.0
31	36.372376487043894	38.0	35.0	40.0	30.0	41.0
32	36.04822767917675	38.0	35.0	40.0	30.0	41.0
33	35.548566657389365	38.0	34.0	40.0	28.0	41.0
34	35.621892997066944	38.0	34.0	40.0	28.0	41.0
35	35.840180693228284	38.0	35.0	40.0	29.0	41.0
36	35.70879273801623	38.0	34.0	40.0	29.0	41.0
37	35.68336580387791	38.0	34.0	40.0	28.0	41.0
38	35.63069929421168	38.0	34.0	40.0	27.0	41.0
39	35.140401207476074	38.0	33.0	40.0	26.0	41.0
40	34.56139073840159	37.0	33.0	40.0	24.0	41.0
41	34.72057490704861	38.0	33.0	40.0	25.0	41.0
42	34.65433499611067	38.0	33.0	40.0	24.0	41.0
43	33.22421803078636	37.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	11.0
10	16.0
11	14.0
12	27.0
13	23.0
14	10.0
15	18.0
16	16.0
17	25.0
18	21.0
19	29.0
20	45.0
21	91.0
22	172.0
23	282.0
24	520.0
25	831.0
26	1260.0
27	1859.0
28	2565.0
29	3359.0
30	4235.0
31	5334.0
32	6454.0
33	7908.0
34	9795.0
35	12433.0
36	15956.0
37	21021.0
38	29159.0
39	16636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.43347106553341	27.920386506526224	21.252149835506362	33.39399259243401
2	6.952264731279483	16.23884048042133	48.560948282629326	28.24794650566986
3	8.532973659608784	32.36563974109201	43.39920215233324	15.702184446965967
4	5.598492795820934	24.554868083952414	17.122324748264077	52.72431437196258
5	11.134185417514113	36.49974665838846	31.176718262718815	21.189349661378607
6	23.380219372426442	35.698330799917215	13.769651815852763	27.151798011803578
7	15.693620786857638	39.12664939661878	9.621272131709093	35.558457684814485
8	26.96839295781684	27.233152782832715	27.241002804598686	18.55745145475176
9	11.810714566072205	10.49333818607406	8.916197449456565	68.77974979839718
10	12.196079270947068	26.112740585326165	25.71167583691937	35.9795043068074
11	21.30281815781398	15.73001634231804	23.80412054778879	39.16304495207918
12	11.224817486993942	29.037230512320967	13.041027068302323	46.696924932382764
13	37.74932739586232	9.40289879894667	23.325982858407016	29.521790946783987
14	23.319560113325768	29.925710248560232	11.54809565608341	35.20663398203059
15	30.922663012838353	24.95878738572866	9.337957709791832	34.78059189164115
16	39.887387869575456	18.549601432985792	9.479971739921643	32.083038957517104
17	12.866185674423916	46.209509944550305	9.71904058461253	31.205263796413252
18	34.010576120233786	10.167205463615149	11.011439622628044	44.81077879352302
19	30.590107545298196	26.88846546347242	11.585918488228536	30.935508503000847
20	12.472970947783082	16.771928322164893	16.288795164386592	54.46630556566543
21	33.129232767418124	12.723458005951743	23.292441856316056	30.85486737031407
22	11.236235700471715	48.229106453431534	11.844255568163167	28.69040227793359
23	26.05350860291022	22.910645343152996	20.17812413025327	30.857721923683517
24	36.18217759603788	24.327931091081663	24.51276342175312	14.977127891127335
25	23.415901289544486	34.504413853147504	13.300077786579317	28.779607070728698
26	36.709556331042556	29.70590963911309	22.10637493131231	11.478159098532045
27	14.259207718712311	37.17484853026183	17.985827142520712	30.580116608505143
28	11.227672040363384	20.574193410263547	38.500074932025946	29.698059617347123
29	24.6590592819371	13.920229506090903	24.230162638178225	37.19054857379378
30	16.230276820313	24.43354956575107	29.35694048969863	29.979233124237297
31	15.71217538375902	17.92516788342004	39.4071092651666	26.95554746765434
32	33.27981045765627	18.574578774968423	23.159705124636936	24.985905642738373
33	10.795920843235066	41.5237605886089	33.36045159034305	14.319866977812984
34	14.50826750019625	35.61911694391517	32.55404026347527	17.31857529241331
35	29.004403148572365	13.19374567356755	46.60129739450641	11.200553783353671
36	22.902081683044667	28.927330207597397	13.88526122731522	34.28532688204272
37	17.08307463943423	24.94308734219672	30.50946641261142	27.46437160575763
38	18.341932675358784	11.027853304502344	33.49604287539161	37.13417114474726
39	35.13883833950631	15.59014322721531	20.347970055735153	28.923048377543232
40	12.124001798368623	9.883177403355528	56.6000842093244	21.39273658895145
41	21.89085615191933	21.504064170359747	18.642374417492704	37.96270526022822
42	23.586460853368727	10.898684764535028	46.594874649425165	18.919979732671077
43	23.789134142599213	11.211971996831446	39.59336887252278	25.405524988046558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	15.0
4	26.0
5	26.0
6	46.0
7	66.0
8	74.0
9	82.0
10	135.0
11	188.0
12	188.0
13	281.5
14	375.0
15	897.0
16	1419.0
17	1391.0
18	1363.0
19	1363.0
20	1157.0
21	951.0
22	740.0
23	529.0
24	553.0
25	577.0
26	577.0
27	634.5
28	692.0
29	656.0
30	620.0
31	626.5
32	633.0
33	633.0
34	748.5
35	864.0
36	875.5
37	887.0
38	1030.0
39	1173.0
40	1173.0
41	1421.5
42	1670.0
43	2086.5
44	2503.0
45	3224.5
46	3946.0
47	3946.0
48	6883.5
49	9821.0
50	26139.0
51	42457.0
52	25740.5
53	9024.0
54	9024.0
55	21071.0
56	33118.0
57	26281.5
58	19445.0
59	11409.5
60	3374.0
61	3374.0
62	2616.5
63	1859.0
64	1484.0
65	1109.0
66	890.5
67	672.0
68	672.0
69	507.5
70	343.0
71	265.5
72	188.0
73	130.0
74	72.0
75	72.0
76	54.0
77	36.0
78	28.0
79	20.0
80	15.0
81	10.0
82	10.0
83	9.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	140127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.30073433385429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6919523161325	13.095263582321751
2	9.611021738233717	3.3255546754016
3	3.9434063440993277	2.046714765890942
4	2.4213174937095245	1.6756228278632954
5	1.4932145361547662	1.2916853996731537
6	1.1302231572000165	1.1732214348412513
7	0.8208555046817637	0.9940982109086757
8	0.5651115786000083	0.7821476232275008
9	0.5774862847007384	0.8991843113746815
>10	3.3081714309285153	11.21482655020089
>50	0.2846182403167925	3.537505263082775
>100	0.09487274677226415	3.11788591777459
>500	0.01649960813430681	2.0745466612430152
>1k	0.020624510167883515	7.954212963954127
>5k	0.008249804067153406	11.015007814339848
>10k+	0.012374706100730107	35.802521997901906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	17777	12.686348812148978	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	16719	11.93131944593119	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	15673	11.184853739821733	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	8633	6.16083980960129	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	6802	4.8541680047385585	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	4759	3.396204871295325	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1942	1.385885660864787	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1810	1.2916853996731537	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1497	1.068316598514205	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1138	0.8121204336066569	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.680097340269898	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	835	0.5958880158713167	No Hit
TCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT	603	0.43032392044359763	TruSeq Adapter, Index 8 (95% over 24bp)
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT	516	0.36823738465820294	TruSeq Adapter, Index 8 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.26547346335823935	No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	333	0.23764156800616582	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	324	0.2312188229249181	No Hit
CTGATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	299	0.21337786436589667	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	275	0.19625054414923607	No Hit
TCGCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	216	0.1541458819499454	TruSeq Adapter, Index 8 (95% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	213	0.15200496692286283	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	210	0.14986405189578025	No Hit
CTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	199	0.14201403012981081	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	189	0.13487764670620225	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	168	0.1198912415166242	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	153	0.10918666638121133	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	141	0.10062300627288104	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	141	0.10062300627288104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.136383423608584E-4	0.0
9	0.0	0.0	0.0	0.0014272766847217167	0.0
10	0.0	0.0	0.0	0.0014272766847217167	0.0
11	0.0	0.0	0.0	0.0014272766847217167	0.0
12	0.0	0.0	0.0	0.0014272766847217167	0.0
13	0.0	0.0	0.0	0.0014272766847217167	0.0
14	0.0	0.0	0.0	0.0014272766847217167	0.0
15	0.0	0.0	0.0	0.0014272766847217167	0.0
16	0.0	0.0	0.0	0.0014272766847217167	0.0
17	0.0	0.0	0.0	0.0014272766847217167	0.0
18	0.0	0.0	0.0	0.0014272766847217167	0.0
19	0.0	0.0	0.0	0.0014272766847217167	0.0
20	0.0	0.0	0.0	0.0014272766847217167	0.0
21	0.0	0.0	0.0	0.002140915027082575	0.0
22	0.0	0.0	0.0	0.0028545533694434334	0.0
23	0.0	0.0	0.0	0.003568191711804292	0.0
24	0.0	0.0	0.0	0.00428183005416515	0.0
25	0.0	0.0	0.0	0.00428183005416515	0.0
26	0.0	0.0	0.0	0.0049954683965260085	0.0
27	0.0	0.0	0.0	0.0049954683965260085	0.0
28	0.0	0.0	0.0	0.01355912850485631	0.0
29	0.0	0.0	0.0	0.024977341982630043	0.0
30	0.0	0.0	0.0	0.03925010882984721	0.0
31	0.0	0.0	0.0	0.07850021765969442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTCAA	20	0.0018364252	37.0	28
GGCACAA	20	0.0018364252	37.0	36
CTATACA	20	0.0018364252	37.0	1
GAAGCCA	20	0.0018364252	37.0	20
AGGGCAC	20	0.0018364252	37.0	34
AAGGGCA	20	0.0018364252	37.0	33
TGATACA	25	1.2281003E-4	37.0	2
CGGAAGC	20	0.0018364252	37.0	18
TCGCCGA	20	0.0018364252	37.0	1
TTTACAC	40	3.792411E-8	37.0	2
AAGCCAC	20	0.0018364252	37.0	21
CTCAAGG	20	0.0018364252	37.0	30
CTTATAC	1950	0.0	36.620514	1
CGTCTTC	875	0.0	33.405716	37
TTATACA	2180	0.0	32.587154	2
CAGACAC	35	2.3734774E-5	31.714287	18
GATACAC	30	3.5835087E-4	30.833332	3
GGAAGCC	30	3.5835087E-4	30.833332	19
TATACAC	2400	0.0	29.908333	3
ACGCCTC	25	0.005480088	29.6	26
>>END_MODULE