FastQCFastQC Report
Fri 10 Feb 2017
ERR1631096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999494
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT135711.3577870402423626No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG114251.1430783976692207No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT97760.97809491602751TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC67500.6753417229117934No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC35590.35608017656934404RNA PCR Primer, Index 6 (95% over 22bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG33230.33246822892383543No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC32910.3292666089041055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31170.3118578000468237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29140.2915475230466616No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG19420.19429831494736338No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG16600.16608403852349288No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA16340.16348272225746227No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13020.13026591455276368No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC11950.11956049761179158No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC11040.11045589068068443No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA10850.10855492879396976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10190.10195158750327667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16700.030.4640711
TTATACA17600.028.6960222
TATACAC18700.028.4919783
CCGTCTT15050.027.16611137
GGTATCA6650.026.984961
ACTCGAA358.8685815E-426.42857227
GCCGTCT15750.025.9587336
CGTCTTC4150.025.85542137
GATTACG1150.025.739131
TTACGGG1200.023.1250023
GTATCAA17600.023.1251
TGCCGTC18800.021.7473435
AATCGTC603.72566E-521.58333428
ATGCCGT19750.020.79493734
GATCGGA450.003825464820.55555511
TATGCCG21650.018.96997633
CGAAAAC609.235666E-418.530
ATTCCCG801.6163262E-518.531
AATAGCG609.235666E-418.56
ATCGCCG609.235666E-418.532
Produced by FastQC (version 0.11.5)