##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999494
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394783760582854	31.0	31.0	34.0	28.0	34.0
2	31.386773707495994	31.0	31.0	34.0	28.0	34.0
3	30.85311967855735	31.0	31.0	34.0	27.0	34.0
4	35.037993224571636	37.0	35.0	37.0	32.0	37.0
5	35.23247563267013	37.0	35.0	37.0	32.0	37.0
6	35.428939043155836	37.0	35.0	37.0	33.0	37.0
7	35.43657890892792	37.0	35.0	37.0	33.0	37.0
8	35.46183168683354	37.0	35.0	37.0	33.0	37.0
9	37.23910098509846	39.0	37.0	39.0	34.0	39.0
10	37.05290877183855	39.0	37.0	39.0	33.0	39.0
11	37.11660700314359	39.0	37.0	39.0	33.0	39.0
12	37.03380010285204	39.0	37.0	39.0	33.0	39.0
13	37.12488319089459	39.0	37.0	39.0	33.0	39.0
14	38.2478464102836	40.0	38.0	41.0	33.0	41.0
15	38.248240609748535	40.0	38.0	41.0	33.0	41.0
16	38.249789393433076	40.0	38.0	41.0	33.0	41.0
17	38.22616143768747	40.0	38.0	41.0	33.0	41.0
18	38.25968039828153	40.0	38.0	41.0	33.0	41.0
19	38.30604285768599	40.0	38.0	41.0	34.0	41.0
20	38.29389571122988	40.0	38.0	41.0	34.0	41.0
21	38.244041484991406	40.0	38.0	41.0	34.0	41.0
22	38.175963037296874	40.0	38.0	41.0	33.0	41.0
23	38.17177291709605	40.0	38.0	41.0	33.0	41.0
24	38.13608285792611	40.0	37.0	41.0	33.0	41.0
25	38.10636782211799	40.0	37.0	41.0	33.0	41.0
26	37.97457013248704	40.0	37.0	41.0	33.0	41.0
27	37.93424772935105	40.0	37.0	41.0	33.0	41.0
28	37.904740798844216	40.0	37.0	41.0	33.0	41.0
29	37.82241614256814	40.0	37.0	41.0	33.0	41.0
30	37.75457681586883	40.0	37.0	41.0	33.0	41.0
31	37.692211258897004	40.0	37.0	41.0	33.0	41.0
32	37.62948351866044	40.0	37.0	41.0	32.0	41.0
33	37.569159994957445	39.0	37.0	41.0	32.0	41.0
34	37.50741775338321	39.0	37.0	41.0	32.0	41.0
35	37.496309132421004	39.0	37.0	41.0	32.0	41.0
36	37.44312121933699	39.0	37.0	41.0	32.0	41.0
37	37.3993430675922	39.0	36.0	41.0	31.0	41.0
38	37.379064806792236	39.0	36.0	41.0	31.0	41.0
39	37.288461961752645	39.0	36.0	41.0	31.0	41.0
40	37.20326485201512	39.0	36.0	41.0	31.0	41.0
41	37.156632255921494	39.0	36.0	41.0	31.0	41.0
42	37.08549325958935	39.0	36.0	41.0	31.0	41.0
43	36.44161745843397	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	10.0
11	5.0
12	13.0
13	13.0
14	8.0
15	13.0
16	7.0
17	9.0
18	17.0
19	37.0
20	74.0
21	200.0
22	414.0
23	724.0
24	1369.0
25	2315.0
26	3749.0
27	5819.0
28	8698.0
29	12190.0
30	16814.0
31	22363.0
32	29089.0
33	38221.0
34	51023.0
35	68051.0
36	95773.0
37	141577.0
38	242386.0
39	258506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.951809615665525	22.521695978164953	14.828703323881884	23.697791082287637
2	13.957162324136013	22.8270504875467	40.81595287215331	22.39983431616398
3	14.773875581044008	28.33093545333939	34.09275093197158	22.802438033645025
4	11.542540525505906	19.174602348788486	38.756610845087614	30.52624628061799
5	12.372560515620904	38.266462830192076	36.193613968668146	13.16736268551887
6	27.86930186674457	40.55221942302805	17.62081613296328	13.957662577264095
7	25.096098625904705	33.089743410165546	23.090783936671954	18.72337402725779
8	24.80705236849846	35.21311783762584	22.143804765211197	17.836025028664505
9	24.08938923095086	15.037208827666799	20.23293786656048	40.64046407482186
10	14.457615553470054	29.05310086903973	34.33507354721489	22.15421003027532
11	31.705242852883558	23.687085665346665	25.07999047518044	19.527681006589333
12	20.60832781387382	26.43827776855089	30.341452775104205	22.611941642471088
13	28.51382799696647	21.460258890998844	26.987355601934578	23.038557510100112
14	21.554006327201563	22.36401619219325	27.136030831600788	28.945946649004394
15	22.96702131278427	29.810784256833955	23.83215907249068	23.390035357891094
16	24.272982128957253	28.08481091432265	24.068878852699466	23.573328104020636
17	21.436947095230185	29.907933414307642	25.607957626559042	23.047161863903135
18	23.145511628884215	26.08820062951854	26.848985586706874	23.917302154890375
19	24.071880371467962	27.303415528257297	26.504711383960284	22.119992716314453
20	23.786636037835144	27.229878318429122	26.11001166590295	22.873473977832784
21	23.51619919679358	27.32282534962691	26.312514132150866	22.848461321428644
22	21.996029991175533	28.933740472679176	27.551340978535137	21.51888855761015
23	22.237752302665147	26.63257608349825	26.909816367081746	24.219855246754857
24	22.60443784554985	29.150850330267115	26.486101967595605	21.758609856587434
25	23.06857269778508	28.156447162264104	26.92882598594889	21.846154154001926
26	23.432056620650048	27.52072548709647	26.935729479116432	22.11148841313705
27	22.710491508703402	28.587065054917787	26.808765235209016	21.89367820116979
28	21.51888855761015	27.155940906098486	27.900917864439407	23.42425267185196
29	21.767214210390456	27.759246178566354	27.83898652718275	22.634553083860435
30	23.700192297302436	26.45858804555105	27.355742005454758	22.485477651691756
31	22.009436775008155	28.21037444947143	27.283205301882752	22.49698347363766
32	22.970322983429615	26.948335857944123	28.70452448939163	21.376816669234632
33	21.226640680184172	26.112412880917745	29.642399053921288	23.0185473849768
34	22.10298410995964	29.24819958899203	27.180753461251395	21.468062839796936
35	23.105391328011972	26.350133167382694	29.42959137323486	21.114884131370474
36	22.569420126584053	26.30851210712621	27.183955081271122	23.93811268501862
37	22.133699652023925	27.10541534016212	28.290915203092766	22.469969804721188
38	21.92019161695818	25.532619505469768	28.205772120693073	24.34141675687898
39	23.32870432438814	26.36924283687546	27.39236053443042	22.909692304305977
40	21.595027083704355	25.74412652802318	30.691730015387787	21.969116372884677
41	22.460164843410766	26.602560895813284	26.732126455986727	24.205147804789224
42	22.07527008666385	26.73042559535125	29.10642785249336	22.087876465491536
43	23.07117401405111	24.758327713823196	28.85770199721059	23.312796274915108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	28.5
2	39.0
3	130.5
4	222.0
5	222.0
6	285.0
7	348.0
8	347.0
9	346.0
10	564.5
11	783.0
12	783.0
13	1106.5
14	1430.0
15	3257.5
16	5085.0
17	5801.5
18	6518.0
19	6518.0
20	6786.5
21	7055.0
22	9469.5
23	11884.0
24	15317.5
25	18751.0
26	18751.0
27	22686.0
28	26621.0
29	31422.0
30	36223.0
31	40470.5
32	44718.0
33	44718.0
34	53381.5
35	62045.0
36	64056.0
37	66067.0
38	66410.5
39	66754.0
40	66754.0
41	66813.5
42	66873.0
43	68234.5
44	69596.0
45	69052.5
46	68509.0
47	68509.0
48	67200.5
49	65892.0
50	77286.0
51	88680.0
52	75590.5
53	62501.0
54	62501.0
55	68968.5
56	75436.0
57	63185.0
58	50934.0
59	42186.0
60	33438.0
61	33438.0
62	28558.5
63	23679.0
64	18669.0
65	13659.0
66	11379.0
67	9099.0
68	9099.0
69	7459.0
70	5819.0
71	5493.0
72	5167.0
73	4490.5
74	3814.0
75	3814.0
76	2334.0
77	854.0
78	596.0
79	338.0
80	250.5
81	163.0
82	163.0
83	120.0
84	77.0
85	55.0
86	33.0
87	22.0
88	11.0
89	11.0
90	11.5
91	12.0
92	7.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	999494.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.692941102390456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0455055080621	40.34401719534544
2	13.255278007234047	13.704086106475222
3	3.9183064496886924	6.076463535746227
4	1.567580093300672	3.2413130174508553
5	0.7970620333399927	2.0601240372197913
6	0.4480834115770038	1.3897649642164942
7	0.31448717748600236	1.1379737000268653
8	0.21208241174323164	0.8770530895276636
9	0.1713898095325026	0.7973678996742216
>10	1.0790769080604727	10.961314790572665
>50	0.11031144670094928	3.9314351599621773
>100	0.07250106001179689	6.761659915162284
>500	0.00504018057738881	1.8364185585397887
>1k	0.002520090288694405	2.7201561590943233
>5k	3.877061982606777E-4	1.6560434954943006
>10k+	3.877061982606777E-4	2.504808375491682
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	13571	1.3577870402423626	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	11425	1.1430783976692207	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	9776	0.97809491602751	TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	6750	0.6753417229117934	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	3559	0.35608017656934404	RNA PCR Primer, Index 6 (95% over 22bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	3323	0.33246822892383543	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	3291	0.3292666089041055	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3117	0.3118578000468237	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2914	0.2915475230466616	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1942	0.19429831494736338	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	1660	0.16608403852349288	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1634	0.16348272225746227	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1302	0.13026591455276368	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	1195	0.11956049761179158	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1104	0.11045589068068443	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	1085	0.10855492879396976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.10195158750327667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0005062561656198E-4	0.0
5	0.0	0.0	0.0	1.0005062561656198E-4	0.0
6	0.0	0.0	1.0005062561656198E-4	1.0005062561656198E-4	0.0
7	0.0	0.0	1.0005062561656198E-4	1.0005062561656198E-4	0.0
8	0.0	0.0	1.0005062561656198E-4	1.0005062561656198E-4	0.0
9	0.0	0.0	1.0005062561656198E-4	1.0005062561656198E-4	0.0
10	0.0	0.0	1.0005062561656198E-4	1.0005062561656198E-4	0.0
11	0.0	0.0	1.0005062561656198E-4	2.0010125123312396E-4	0.0
12	0.0	0.0	1.0005062561656198E-4	2.0010125123312396E-4	0.0
13	0.0	0.0	1.0005062561656198E-4	2.0010125123312396E-4	0.0
14	0.0	0.0	2.0010125123312396E-4	2.0010125123312396E-4	0.0
15	0.0	0.0	2.0010125123312396E-4	2.0010125123312396E-4	0.0
16	0.0	0.0	4.002025024662479E-4	2.0010125123312396E-4	0.0
17	0.0	0.0	4.002025024662479E-4	2.0010125123312396E-4	0.0
18	0.0	0.0	4.002025024662479E-4	2.0010125123312396E-4	0.0
19	0.0	0.0	4.002025024662479E-4	3.001518768496859E-4	0.0
20	0.0	0.0	4.002025024662479E-4	7.003543793159338E-4	0.0
21	0.0	0.0	4.002025024662479E-4	0.0011005568817821817	0.0
22	0.0	0.0	4.002025024662479E-4	0.0012006075073987437	0.0
23	0.0	0.0	4.002025024662479E-4	0.0017008606354815537	0.0
24	0.0	0.0	4.002025024662479E-4	0.0024012150147974874	0.0
25	0.0	0.0	4.002025024662479E-4	0.0026013162660306114	0.0
26	0.0	0.0	4.002025024662479E-4	0.0027013668916471734	0.0
27	0.0	0.0	4.002025024662479E-4	0.0035017718965796693	0.0
28	0.0	0.0	4.002025024662479E-4	0.011005568817821818	0.0
29	0.0	0.0	4.002025024662479E-4	0.026113213285922678	0.0
30	0.0	0.0	4.002025024662479E-4	0.04872465467526568	0.0
31	0.0	0.0	4.002025024662479E-4	0.10745437191218757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1670	0.0	30.464071	1
TTATACA	1760	0.0	28.696022	2
TATACAC	1870	0.0	28.491978	3
CCGTCTT	1505	0.0	27.166111	37
GGTATCA	665	0.0	26.98496	1
ACTCGAA	35	8.8685815E-4	26.428572	27
GCCGTCT	1575	0.0	25.95873	36
CGTCTTC	415	0.0	25.855421	37
GATTACG	115	0.0	25.73913	1
TTACGGG	120	0.0	23.125002	3
GTATCAA	1760	0.0	23.125	1
TGCCGTC	1880	0.0	21.74734	35
AATCGTC	60	3.72566E-5	21.583334	28
ATGCCGT	1975	0.0	20.794937	34
GATCGGA	45	0.0038254648	20.555555	11
TATGCCG	2165	0.0	18.969976	33
CGAAAAC	60	9.235666E-4	18.5	30
ATTCCCG	80	1.6163262E-5	18.5	31
AATAGCG	60	9.235666E-4	18.5	6
ATCGCCG	60	9.235666E-4	18.5	32
>>END_MODULE