FastQCFastQC Report
Fri 10 Feb 2017
ERR1631095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275026
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT187296.809901609302393No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG152895.559110774981275No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT128474.671194723407969Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC81062.9473577043624966No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC53971.9623599223346155Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC40101.4580439667522342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39131.4227745740402726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36501.3271472515325824No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21050.7653821820482427No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG18050.6563015860318661No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA15070.5479481939889319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14570.5297680946528692No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT12880.468319358896977No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12780.46468333902976444No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11900.43268636419829404No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10880.39559896155272595No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9760.3548755390399453No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9740.3541483350665028No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9080.33015060394289997No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9030.32833259400929365No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8570.3116069026201159No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8250.2999716390450357No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7920.28797277348323436No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6180.22470602779373589No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6090.22143360991324457No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6050.21997920196635953No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6020.21888839600619578No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5620.20434431653734556No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5480.199253888723248No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5430.19743587878964172No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5410.1967086748161992No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5380.19561786885603544No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.1941634609091504No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5250.1908910430286591No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5250.1908910430286591No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5000.18180099336062772No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4620.16798411786522No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT4560.1658025059448925No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4360.15853046621046737No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4320.15707605826358234No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4230.15380364038309105No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4060.14762240660882972No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4040.1468952026353872No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3900.14180477482128964No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3880.1410775708478471No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3840.13962316290096208No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3780.13744155098063454No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3650.13271472515325825No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3570.12980590925948818No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3370.12253386952506308No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3240.11780704369768677No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.11635263575080174No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3170.11526182979063797No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3160.11489822780391672No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3150.11453462581719548No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3150.11453462581719548No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3140.1141710238304742No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3130.11380742184375295No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3130.11380742184375295No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3100.1127166158835892No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3100.1127166158835892No Hit
CTTTACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA3030.1101714019765404No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2920.1061717801226066No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2920.1061717801226066No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2910.10580817813588533No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2900.10544457614916408No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2850.1036265662155578No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2850.1036265662155578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA403.8151484E-837.01
ACTTGGA200.001839502437.031
ATTACGG308.288995E-637.02
TCTACAC200.001839502437.03
TTAACAC308.288995E-637.02
TTACGGG308.288995E-637.03
ATTACTT251.2311849E-436.9999962
TTACTTT251.2311849E-436.9999963
CTTATAC20250.035.8123441
CGTCTTC5850.033.83760537
TTATACA21600.033.5740742
CGCCGAG507.2886905E-933.32
TCAGAGT352.3817545E-531.7142853
ACTCAGA352.3817545E-531.7142851
GATTACG352.3817545E-531.7142851
CTCAGAG352.3817545E-531.7142852
TATACAC23350.031.0578173
CCTAGTT303.5924243E-430.8333322
GACTTGG303.5924243E-430.83333230
TAGTTCC303.5924243E-430.8333324
Produced by FastQC (version 0.11.5)