FastQCFastQC Report
Fri 10 Feb 2017
ERR1631094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792547
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT159802.0162842077504552No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT137131.730244389291739TruSeq Adapter, Index 3 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG129741.637000707844456No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC67270.8487824696831859No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC61690.7783765505389586RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC38320.4835044483166298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.3114010904085183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23990.3026949821272429No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC20930.26408528453202146No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG15650.1974646298579138No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA13840.17462686755485793No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT10980.13854067960638297No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG10080.1271848861960237No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9950.1255446049256385No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9190.11595526826800176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9000.11355793410359259No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA8340.10523035226932913No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT8310.10485182582231715No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG7980.10068803490518544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18050.032.5927961
GATTACG352.3873796E-531.7142851
CGTCTTC7650.030.95424837
TTATACA19100.030.7041912
GCATTAG250.00549532729.5999981
TATACAC20250.029.3259263
GGTATCA4350.025.0919551
CCGTCTT21000.023.96190537
GCCGTCT21250.023.59294136
GTATCAA11950.023.2217581
TCGCCGA802.7230271E-823.1251
TAGAGTG400.001930661223.1255
CGAGTCA400.001930661223.12515
TTGACGC400.001930661223.12532
GGCGCTA400.001930661223.12518
GCGCTAT450.003824775820.55555519
GCAACGC555.141158E-420.1818183
CGCCTGT555.141158E-420.18181810
TGCCGTC25500.019.66078435
AACGGCC951.6744707E-719.47368437
Produced by FastQC (version 0.11.5)