FastQCFastQC Report
Fri 10 Feb 2017
ERR1631093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250623
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT135035.387773668019296No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG107904.305271264010087No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT84643.377184057329136RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC61592.4574759698830517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38041.5178175985444273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37401.4922812351619763No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC30761.227341465069048No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC29751.1870418916061176RNA PCR Primer, Index 41 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA18000.7182102201314324No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14790.590129397541327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13930.5558149092461585No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG13600.5426477218770823No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA11170.445689342159339No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10330.4121728652198721No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9780.3902275529380783No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT8980.35830709871001465No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG8600.34314488295168444No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8170.3259876388041002No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7860.3136184627907255No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7380.2944661902538873No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7290.29087513915323016No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT6950.2773089461063031No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6890.2749149120391983No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6210.24778252594534422No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6050.24139843509973147No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5690.22703423069710282No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5510.2198521284957885No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5360.21386704332802656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.20628593544886145No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5050.20149786731465189No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4990.19910383324754713No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4940.19710880485829313No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4900.19551278214688994No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4860.19391675943548678No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4790.1911237196905312No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4760.1899267026569788No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4740.1891286913012772No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4670.18633565155632165No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4410.17596150393220095No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4230.16877940173088662No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3990.15920326546246755No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3880.15481420300610876No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3760.15002613487189922No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3760.15002613487189922No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3690.14723309512694366No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3690.14723309512694366No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3530.14084900428133093No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3520.14044999860348015No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.13925298156992774No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3440.13725795318067377No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3370.1344649134357182No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3330.13286889072431501No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3190.12728281123440388No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT3060.12209573742234352TruSeq Adapter, Index 3 (95% over 23bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3040.12129772606664192No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3010.12010070903308953No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2950.11770667496598478No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC2920.11650965793243237No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2850.1137166181874768No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2780.11092357844252124No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2780.11092357844252124No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2760.11012556708681964No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2740.10932755573111806No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2740.10932755573111806No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2740.10932755573111806No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2730.10892855005326725No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2720.10852954437541647No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2700.10773153301971486No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2680.10693352166401328No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2630.10493849327475929No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA2560.10214545352980373No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2540.10134744217410213No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2510.10015042514054974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTG200.001839191437.03
TGATACA200.001839191437.02
GATACAC200.001839191437.03
GATATGT200.001839191437.01
ATTACTT251.2308727E-436.9999962
TTACTTT251.2308727E-436.9999963
CTTATAC15400.035.438311
TTATACA16300.033.3680952
TATACAC16700.032.5688633
GACCGTT401.5934656E-632.3757
TAGGACC401.5934656E-632.3754
TTAGGAC303.5915204E-430.8333343
GCCTCGC303.5915204E-430.83333435
TTCGGCC303.5915204E-430.83333431
GGTATCA4250.030.0352951
ATTGTTA250.00548824729.59999814
TTATCAA250.00548824729.5999982
TATTGTT250.00548824729.59999813
CGTTATT250.00548824729.59999810
AGCGGGT250.00548824729.5999987
Produced by FastQC (version 0.11.5)