##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250623
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148829117838346	31.0	31.0	34.0	28.0	34.0
2	31.141056487233815	31.0	31.0	34.0	28.0	34.0
3	30.57782007237963	31.0	30.0	34.0	26.0	34.0
4	34.772506912773366	35.0	35.0	37.0	32.0	37.0
5	35.005610019830584	35.0	35.0	37.0	32.0	37.0
6	35.21237077203609	36.0	35.0	37.0	32.0	37.0
7	35.20259912298552	37.0	35.0	37.0	32.0	37.0
8	35.22607262701348	37.0	35.0	37.0	32.0	37.0
9	37.01024646580721	39.0	37.0	39.0	33.0	39.0
10	36.840665062663845	39.0	37.0	39.0	32.0	39.0
11	36.87915315034933	39.0	37.0	39.0	33.0	39.0
12	36.76303451798119	39.0	37.0	39.0	32.0	39.0
13	36.84941525717911	39.0	37.0	39.0	32.0	39.0
14	37.718649126377066	39.0	37.0	41.0	32.0	41.0
15	37.72249953116833	39.0	37.0	41.0	32.0	41.0
16	37.71445158664608	39.0	37.0	41.0	32.0	41.0
17	37.736444779609215	39.0	37.0	41.0	32.0	41.0
18	37.846857630784086	39.0	37.0	41.0	33.0	41.0
19	37.82308487249774	40.0	37.0	41.0	32.0	41.0
20	37.91646816134194	40.0	37.0	41.0	33.0	41.0
21	37.80054903181272	39.0	37.0	41.0	33.0	41.0
22	37.63081600651177	39.0	37.0	41.0	32.0	41.0
23	37.67528917936502	39.0	37.0	41.0	32.0	41.0
24	37.51431033863612	39.0	36.0	41.0	32.0	41.0
25	37.45249637902347	39.0	36.0	41.0	32.0	41.0
26	37.28377682814426	39.0	36.0	40.0	32.0	41.0
27	37.153625166086115	39.0	36.0	40.0	31.0	41.0
28	37.16699983640767	39.0	36.0	40.0	31.0	41.0
29	37.01823455947778	39.0	35.0	40.0	31.0	41.0
30	36.804690710748815	39.0	35.0	40.0	31.0	41.0
31	36.742788969887044	38.0	35.0	40.0	31.0	41.0
32	36.48084972249155	38.0	35.0	40.0	30.0	41.0
33	36.37473017241035	38.0	35.0	40.0	30.0	41.0
34	36.26118512666435	38.0	35.0	40.0	30.0	41.0
35	36.20543605335504	38.0	35.0	40.0	30.0	41.0
36	36.09773245073277	38.0	35.0	40.0	30.0	41.0
37	35.977021263012574	38.0	35.0	40.0	30.0	41.0
38	35.86628920729542	38.0	35.0	40.0	29.0	41.0
39	35.657852631242946	38.0	35.0	40.0	28.0	41.0
40	35.344062596010744	38.0	34.0	40.0	26.0	41.0
41	35.251684801474724	38.0	34.0	40.0	26.0	41.0
42	35.03078727810297	38.0	34.0	40.0	26.0	41.0
43	34.050390427055774	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	11.0
11	12.0
12	7.0
13	11.0
14	3.0
15	11.0
16	9.0
17	13.0
18	16.0
19	29.0
20	49.0
21	132.0
22	221.0
23	380.0
24	703.0
25	1058.0
26	1704.0
27	2486.0
28	3571.0
29	4842.0
30	6260.0
31	7871.0
32	9921.0
33	12488.0
34	16161.0
35	20900.0
36	27916.0
37	39190.0
38	54674.0
39	39969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.39330787677109	21.7609716586267	16.386764183654336	25.458956280947874
2	13.431728133491339	21.561069814023455	41.66536989821365	23.341832154271554
3	15.093985787417754	29.92622385016539	35.452053482721055	19.527736879695798
4	10.420033277073532	20.319763150229626	30.31804742581487	38.94215614688197
5	13.808389493382492	37.18613215866062	30.567425974471618	18.438052373485274
6	26.365896186702738	38.008482860711105	15.366107659711997	20.259513292874157
7	22.66232548489165	32.77711941840932	16.482126540660673	28.078428556038354
8	27.352238222349907	29.677643312864344	23.12237903145362	19.847739433332137
9	22.30202335779238	11.545628294290628	15.290695586598197	50.8616527613188
10	16.51484500624444	27.40650299453761	27.194630979598838	28.884021019619112
11	31.878159626211485	19.946293835761285	22.935245368541594	25.24030116948564
12	20.707995674778452	25.970082554274747	22.736540540971898	30.5853812299749
13	34.89943061889771	17.476049684187007	24.565981573917796	23.05853812299749
14	23.60118584487457	23.005869373521186	23.782733428296684	29.61021135330756
15	28.268355258695333	24.239195923757993	19.832577217573807	27.659871599972867
16	27.872541626267346	21.99000091771306	19.350179353052194	30.787278102967402
17	20.087142840042613	31.592072555192463	20.067591561827925	28.253193042937003
18	27.251289785853654	17.31644741304669	23.356196358674183	32.07606644242548
19	25.616962529376792	23.255646927855782	23.17305275254067	27.95433779022676
20	22.10052549047773	19.224891570207046	24.901944354668167	33.772638584647055
21	27.974687079797146	19.467487022340325	27.10964277021662	25.44818312764591
22	19.567238441803028	36.55211213655571	22.90851198812559	20.972137433515677
23	25.71511792612809	21.92456398654553	25.09107304596944	27.26924504135694
24	28.691700282895027	24.810173048762486	23.745625900256563	22.75250076808593
25	19.772726365896187	30.71465906959856	23.853756438954125	25.658858125551127
26	26.683903711949817	21.398674503138178	25.43381892324328	26.48360286166872
27	19.968239148043075	32.27317524728377	25.030025177258274	22.72856042741488
28	17.997151099460147	22.73255048419339	31.675464741863273	27.594833674483187
29	22.02591142871963	25.359603867163028	23.062927185453848	29.55155751866349
30	18.13600507535222	27.19502998527669	29.295395873483283	25.373569065887807
31	27.773189212482496	18.485534049149518	28.234040770400163	25.507235967967823
32	20.923059735140033	32.06728831751276	26.82834376733181	20.1813081800154
33	19.270777223159886	25.978461673509614	30.20871987008375	24.54204123324675
34	24.048471209745315	27.11403183267298	25.32888042996852	23.50861652761319
35	19.73482082650036	26.311232408837178	34.650850081596666	19.303096683065803
36	23.19459905914461	20.572333744309184	24.24717603731501	31.985891159231194
37	22.513496367053303	25.3177082709887	28.634642470962362	23.53415289099564
38	21.078272943823993	19.150676514126797	30.735806370524653	29.03524417152456
39	27.077323310310707	20.081955766230553	26.097764371187004	26.74295655227174
40	19.550879209011143	19.892827074929276	38.38594223195796	22.17035148410162
41	24.413561404978793	21.286952913339956	24.735558987004385	29.563926694676866
42	24.73795302107149	18.853816289805803	33.42151358813836	22.986717100984347
43	24.531667085622626	19.124342139388645	31.774418149970273	24.569572625018456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	61.0
4	117.0
5	117.0
6	169.0
7	221.0
8	244.0
9	267.0
10	407.5
11	548.0
12	548.0
13	957.5
14	1367.0
15	3463.0
16	5559.0
17	5736.5
18	5914.0
19	5914.0
20	4751.5
21	3589.0
22	2739.5
23	1890.0
24	1803.0
25	1716.0
26	1716.0
27	1695.0
28	1674.0
29	1664.5
30	1655.0
31	1743.0
32	1831.0
33	1831.0
34	2150.5
35	2470.0
36	2446.5
37	2423.0
38	2748.0
39	3073.0
40	3073.0
41	4023.5
42	4974.0
43	6283.5
44	7593.0
45	10712.0
46	13831.0
47	13831.0
48	19720.5
49	25610.0
50	36478.5
51	47347.0
52	36982.5
53	26618.0
54	26618.0
55	32864.0
56	39110.0
57	32317.5
58	25525.0
59	17867.5
60	10210.0
61	10210.0
62	8542.0
63	6874.0
64	5414.0
65	3954.0
66	3220.5
67	2487.0
68	2487.0
69	1914.0
70	1341.0
71	960.0
72	579.0
73	361.0
74	143.0
75	143.0
76	99.0
77	55.0
78	40.0
79	25.0
80	20.0
81	15.0
82	15.0
83	9.0
84	3.0
85	3.0
86	3.0
87	3.5
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	250623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.183303208404656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.13743476198007	14.55971718477554
2	10.813696030365334	4.365122115687706
3	4.4974695555907	2.7232137513316816
4	2.5047445832674367	2.022160775347833
5	1.640835046655069	1.6558735630808026
6	1.2098687331962676	1.4651488490681221
7	0.8896093626443144	1.2568678852300068
8	0.6247034635457852	1.0086863536068118
9	0.5139965206389372	0.9336732861708621
>10	4.1495334493120355	17.34278178778484
>50	0.5060888818598767	7.111478196334733
>100	0.45271232010121776	16.91185565570598
>500	0.03163055511624229	4.4265689900767295
>1k	0.019769096947651433	8.68914664655678
>5k	0.003953819389530286	5.834660027212188
>10k+	0.003953819389530286	9.693044932029382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	13503	5.387773668019296	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	10790	4.305271264010087	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	8464	3.377184057329136	RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	6159	2.4574759698830517	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3804	1.5178175985444273	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3740	1.4922812351619763	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	3076	1.227341465069048	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	2975	1.1870418916061176	RNA PCR Primer, Index 41 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1800	0.7182102201314324	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1479	0.590129397541327	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.5558149092461585	No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	1360	0.5426477218770823	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA	1117	0.445689342159339	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1033	0.4121728652198721	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	978	0.3902275529380783	No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT	898	0.35830709871001465	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	860	0.34314488295168444	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	817	0.3259876388041002	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	786	0.3136184627907255	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	738	0.2944661902538873	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	729	0.29087513915323016	No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT	695	0.2773089461063031	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	689	0.2749149120391983	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	621	0.24778252594534422	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	605	0.24139843509973147	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	569	0.22703423069710282	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	551	0.2198521284957885	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	536	0.21386704332802656	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.20628593544886145	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	505	0.20149786731465189	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	499	0.19910383324754713	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	494	0.19710880485829313	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	490	0.19551278214688994	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	486	0.19391675943548678	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	479	0.1911237196905312	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	476	0.1899267026569788	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	474	0.1891286913012772	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	467	0.18633565155632165	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	441	0.17596150393220095	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	423	0.16877940173088662	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	399	0.15920326546246755	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	388	0.15481420300610876	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	376	0.15002613487189922	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	376	0.15002613487189922	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	369	0.14723309512694366	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	369	0.14723309512694366	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	353	0.14084900428133093	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	352	0.14044999860348015	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.13925298156992774	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	344	0.13725795318067377	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	337	0.1344649134357182	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	333	0.13286889072431501	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	319	0.12728281123440388	No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT	306	0.12209573742234352	TruSeq Adapter, Index 3 (95% over 23bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	304	0.12129772606664192	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	301	0.12010070903308953	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	295	0.11770667496598478	No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC	292	0.11650965793243237	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	285	0.1137166181874768	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	278	0.11092357844252124	No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA	278	0.11092357844252124	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	276	0.11012556708681964	No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC	274	0.10932755573111806	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	274	0.10932755573111806	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	274	0.10932755573111806	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	273	0.10892855005326725	No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT	272	0.10852954437541647	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	270	0.10773153301971486	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	268	0.10693352166401328	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	263	0.10493849327475929	No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA	256	0.10214545352980373	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	254	0.10134744217410213	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	251	0.10015042514054974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.990056778507958E-4	0.0
5	0.0	0.0	0.0	3.990056778507958E-4	0.0
6	0.0	0.0	0.0	3.990056778507958E-4	0.0
7	0.0	0.0	0.0	3.990056778507958E-4	0.0
8	0.0	0.0	0.0	3.990056778507958E-4	0.0
9	0.0	0.0	0.0	3.990056778507958E-4	0.0
10	0.0	0.0	0.0	3.990056778507958E-4	0.0
11	0.0	0.0	0.0	3.990056778507958E-4	0.0
12	0.0	0.0	0.0	3.990056778507958E-4	0.0
13	0.0	0.0	0.0	3.990056778507958E-4	0.0
14	0.0	0.0	0.0	3.990056778507958E-4	0.0
15	0.0	0.0	0.0	3.990056778507958E-4	0.0
16	0.0	0.0	0.0	3.990056778507958E-4	0.0
17	0.0	0.0	0.0	3.990056778507958E-4	0.0
18	0.0	0.0	0.0	3.990056778507958E-4	0.0
19	0.0	0.0	0.0	3.990056778507958E-4	0.0
20	0.0	0.0	0.0	7.980113557015916E-4	0.0
21	0.0	0.0	0.0	0.0011970170335523875	0.0
22	0.0	0.0	0.0	0.002394034067104775	0.0
23	0.0	0.0	0.0	0.0027930397449555705	0.0
24	0.0	0.0	0.0	0.00478806813420955	0.0
25	0.0	0.0	0.0	0.0051870738120603456	0.0
26	0.0	0.0	0.0	0.005586079489911141	0.0
27	0.0	0.0	0.0	0.0095761362684191	0.0
28	0.0	0.0	0.0	0.023541334993196952	0.0
29	0.0	0.0	0.0	0.05625980057696221	0.0
30	0.0	0.0	0.0	0.10054943081840055	0.0
31	0.0	0.0	0.0	0.18434062316706767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGTG	20	0.0018391914	37.0	3
TGATACA	20	0.0018391914	37.0	2
GATACAC	20	0.0018391914	37.0	3
GATATGT	20	0.0018391914	37.0	1
ATTACTT	25	1.2308727E-4	36.999996	2
TTACTTT	25	1.2308727E-4	36.999996	3
CTTATAC	1540	0.0	35.43831	1
TTATACA	1630	0.0	33.368095	2
TATACAC	1670	0.0	32.568863	3
GACCGTT	40	1.5934656E-6	32.375	7
TAGGACC	40	1.5934656E-6	32.375	4
TTAGGAC	30	3.5915204E-4	30.833334	3
GCCTCGC	30	3.5915204E-4	30.833334	35
TTCGGCC	30	3.5915204E-4	30.833334	31
GGTATCA	425	0.0	30.035295	1
ATTGTTA	25	0.005488247	29.599998	14
TTATCAA	25	0.005488247	29.599998	2
TATTGTT	25	0.005488247	29.599998	13
CGTTATT	25	0.005488247	29.599998	10
AGCGGGT	25	0.005488247	29.599998	7
>>END_MODULE