FastQCFastQC Report
Fri 10 Feb 2017
ERR1631092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308881
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT162625.264810719986015No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG131454.25568422790654No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT119263.8610338609367365RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC63842.0668153755005974No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC54171.7537498259847641RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49691.6087101505110384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44821.451044253288483No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC37071.2001385646899616No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25250.8174669209177645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18990.614799874385281No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16560.5361288004118091No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14770.4781776800774408No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG14750.47753018152621884No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA14430.467170204706667No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12730.4121328278527977No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12660.4098665829235207No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT11380.3684266756453133No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11180.3619516901330933No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG10840.35094421476231946No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10790.3493254683842645No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9530.308533059657279No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9250.29946807994017116No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8780.28425186398645436No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7730.25025819004729977No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7700.2492869422204668No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7420.2402219625033589No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7260.23504197409358296No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6910.2237107494471981No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.22079700596669916No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6650.21529326828131223No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6430.2081707842178703No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6350.20558079001298232No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6280.20331454508370536No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6270.20299079580809437No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6060.1961920610202635No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5970.1932783175397645No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5920.1916595711617095No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5650.18291834072021265No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5530.17903334941288068No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5460.17676710448360372No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5410.17514835810554874No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5070.16414088273477487No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5040.1631696349079419No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5030.1628458856323309No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5010.1621983870811089No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4840.15669464939572197No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4840.15669464939572197No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4790.155075903017667No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4710.15248590881277904No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4660.15086716243472406No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4650.15054341315911304No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4390.14212593199322718No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4340.1405071856151722No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4170.13500344792978528No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4170.13500344792978528No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4160.13467969865417426No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4130.13370845082734129No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4110.13306095227611928No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4110.13306095227611928No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4030.13047095807123132No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4000.12949971024439833No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3900.12626221748828836No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3890.12593846821267737No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3880.12561471893706638No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.12140597835412342No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3750.12140597835412342No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3660.11849223487362447No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3630.11752098704679148No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3530.11428349429068152No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3460.11201724936140454No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3460.11201724936140454No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3430.11104600153457157No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3420.11072225225896057No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3360.1087797566052946No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3310.10716101022723963No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3300.10683726095162861No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3280.10618976240040663No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3200.10359976819551867No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3130.10133352326624169No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3110.10068602471501971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG308.292302E-637.01
CGGAATC200.00183985337.033
GACAGAC308.292302E-637.016
TGAAGTC200.00183985337.027
TTTACAC308.292302E-637.02
ATTACGG700.037.02
AAAACGG200.00183985337.026
TCAGAGT403.817877E-837.03
ACTCCAT200.00183985337.021
CGACTCC200.00183985337.019
ACTCAGA403.817877E-837.01
AGTCGGA200.00183985337.030
CAGACAC308.292302E-637.018
TTACTTT251.2315364E-436.9999963
CTTATAC18300.035.1803251
TTACGGG750.034.5333373
GATTACG750.034.5333371
CGTCTTC4800.034.30208637
TTATACA19950.032.456142
GACACAT401.5950554E-632.37520
Produced by FastQC (version 0.11.5)