FastQCFastQC Report
Fri 10 Feb 2017
ERR1631091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317900
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG174295.482541679773513No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT158234.977351368354829No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT121163.8112614029569043TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC98023.083359547027367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47831.5045611827618748No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC45711.4378735451399811No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC45431.4290657439446366Illumina Paired End PCR Primer 2 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44851.4108210128971375No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA24300.764391318024536No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG22560.7096571248820384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18110.5696759987417427No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16050.5048757470902799No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA15770.4960679458949355No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT14740.4636678200692041No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT13290.4180559924504561No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12570.39540736080528466No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11060.3479081472161057No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10470.32934885184020135No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10380.32651777288455486No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9660.30386914123938347No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9610.30229631959735764No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9590.30166719094054734No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8630.2714690154136521No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7590.23875432525951557No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7180.22585718779490407No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6930.2179930795847751No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6430.20226486316451717No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6410.20163573450770683No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6330.19911921988046555No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6330.19911921988046555No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6050.1903114186851211No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6050.1903114186851211No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5860.1843346964454231No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5850.18402013211701793No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5780.18181818181818182No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5770.18150361748977664No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5640.1774142812205096No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5570.1752123309216735No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5550.17458320226486315No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5500.17301038062283738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.16955017301038064No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5380.16923560868197546No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5130.16137150047184648No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4860.1528782636049072No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4830.15193457061969173No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4700.14784523435042465No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4690.1475306700220195No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4400.13840830449826988No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.13526266121421832No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4260.13400440390059767No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4190.13180245360176157No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4030.126769424347279No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4010.1261402956904687No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3980.12519660270525323No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3920.12330921673482226No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3880.12205095942120164No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3840.120792702107581No Hit
ATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT3750.11796162315193456No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3740.1176470588235294No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3650.11481597986788299No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3560.11198490091223655No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3510.11041207927021077No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3480.10946838628499529No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3470.10915382195659012No Hit
CTGATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT3470.10915382195659012No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3440.10821012897137465No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3430.10789556464296948No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3400.10695187165775401No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3400.10695187165775401No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3380.10632274300094369No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3350.10537905001572821No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3210.10097514941805599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA200.001839933637.02
GCAACGC355.614238E-737.03
GACCGTT650.037.07
ATTACGG403.8184226E-837.02
TCAGAGT355.614238E-737.03
GCCTAGT308.293069E-637.01
TAGGACC650.037.04
ACTCAGA355.614238E-737.01
GATTACG403.8184226E-837.01
TCGCCGA251.2316178E-436.9999961
ATTGTTA501.7826096E-1036.99999614
TTGTATC251.2316178E-436.9999962
TATTGTT501.7826096E-1036.99999613
CGTTATT501.7826096E-1036.99999610
GTTGTAT251.2316178E-436.9999961
CTTATAC18150.035.4710731
TTAGGAC700.034.3571433
CTTAGGA651.8189894E-1234.1538472
TGTTACG554.965841E-1033.63636416
TTGTTAC554.965841E-1033.63636415
Produced by FastQC (version 0.11.5)