FastQCFastQC Report
Fri 10 Feb 2017
ERR1631090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136335
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG92636.794293468295008No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT87546.420948399163824TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT46893.4393222576741116No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC45953.37037444530018No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC42613.1253896651630177RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC27472.0148897935233068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22131.6232075402501192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17851.309274947739025No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG10270.7532915245534896No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA9050.6638060659405142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8520.6249312355594675No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT8310.6095280008801849No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8010.587523379909781No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6170.45256170462463785No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5960.4371584699453552No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5840.4283566215571937No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4910.3601422965489419No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4230.31026515568269336No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4040.29632889573477095No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3920.28752704734660944No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3710.2721238126673268No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3680.26992335057028644No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.23178200755491987No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3040.22298015916675834No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2960.21711226024131733No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT2880.21124436131587632TruSeq Adapter, Index 10 (95% over 23bp)
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2850.20904389921883598No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2750.20170902556203468No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2710.19877507609931416No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2540.18630579088275204No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2370.1738365056661899No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2290.1679686067407489No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2180.1599002457182675No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2140.15696629625554698No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2110.15476583415850662No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2100.15403234679282649No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2100.15403234679282649No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2090.15329885942714638No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2000.14669747313602524No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1910.1400960868449041No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1900.13936259947922397No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1890.13862911211354384No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1840.13496167528514322No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1810.13276121318810283No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1790.13129423845674257No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1770.12982726372538234No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1720.12615982689698169No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1710.12542633953130156No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1710.12542633953130156No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1680.1232258774342612No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.12175890270290095No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA1590.11662449114314005No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1570.1151575164117798No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1550.11369054168041955No Hit
CTGATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT1540.11295705431473943No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1530.1122235669490593No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1520.11149007958337917No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1490.1092896174863388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1480.10855613012065866No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1470.10782264275497856No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA1450.10635566802361829No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1440.10562218065793816No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT1400.10268823119521765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCG251.227925E-437.05
CTTATAC5450.035.64221
CGTCTTC5600.033.3660737
TTTACAC352.3730086E-531.7142852
GTCCGGC303.5830028E-430.83333223
GCGGTCC303.5830028E-430.83333220
CGGTCCG303.5830028E-430.83333221
TTATACA6450.030.1162782
GTATCTA250.005479571429.63
CTCAGAG250.005479571429.62
GGGAACC250.005479571429.618
GGTGCTT250.005479571429.633
GGTATCA3200.026.5937521
CTATACA358.8291813E-426.428571
GGTCCGG358.8291813E-426.4285722
AACTCAA358.8291813E-426.4285734
TCCGGCC358.8291813E-426.4285724
TATACAC7600.026.0460533
TATGTGC451.3148715E-424.6666664
GTCTTCT601.3230147E-624.66666637
Produced by FastQC (version 0.11.5)