##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631090.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136335
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.22020024205083	31.0	30.0	31.0	26.0	34.0
2	30.209850735321083	31.0	30.0	33.0	26.0	34.0
3	29.632544834415228	31.0	30.0	33.0	25.0	34.0
4	33.810620897055045	35.0	33.0	37.0	28.0	37.0
5	33.67281329079107	35.0	33.0	37.0	28.0	37.0
6	33.95230131660982	35.0	33.0	37.0	30.0	37.0
7	33.98959914915466	35.0	33.0	37.0	30.0	37.0
8	34.056353834305206	35.0	33.0	37.0	30.0	37.0
9	35.70420655004217	37.0	35.0	39.0	30.0	39.0
10	35.414581728829724	37.0	35.0	39.0	30.0	39.0
11	35.47841713426486	37.0	35.0	39.0	30.0	39.0
12	35.312025525360326	37.0	34.0	39.0	30.0	39.0
13	35.51662449114314	37.0	35.0	39.0	30.0	39.0
14	35.9118934976345	38.0	34.0	40.0	29.0	41.0
15	35.95213261451571	38.0	34.0	40.0	29.0	41.0
16	35.99270180071148	38.0	34.0	40.0	29.0	41.0
17	35.97273627461767	38.0	34.0	40.0	29.0	41.0
18	36.221058422268676	38.0	35.0	40.0	30.0	41.0
19	36.08446107015806	38.0	34.0	40.0	29.0	41.0
20	36.096277551619174	38.0	34.0	40.0	30.0	41.0
21	35.876165328052224	38.0	34.0	40.0	29.0	41.0
22	35.596039168225325	38.0	34.0	40.0	27.0	41.0
23	35.65819488759306	38.0	34.0	40.0	28.0	41.0
24	35.49007958337918	38.0	34.0	40.0	27.0	41.0
25	35.4659478490483	38.0	34.0	40.0	27.0	41.0
26	35.09121648879598	37.0	33.0	40.0	27.0	41.0
27	34.759724208750505	37.0	33.0	40.0	26.0	40.0
28	34.67382550335571	37.0	33.0	39.0	25.0	40.0
29	34.70248285473283	37.0	33.0	39.0	26.0	40.0
30	34.43434187846114	37.0	33.0	39.0	25.0	40.0
31	34.304727326071806	37.0	33.0	39.0	25.0	40.0
32	33.8794806909451	36.0	32.0	39.0	24.0	40.0
33	33.68092566105549	36.0	31.0	39.0	23.0	40.0
34	33.47881321744233	36.0	31.0	39.0	23.0	40.0
35	33.44461803645432	36.0	31.0	39.0	23.0	40.0
36	33.47368614075623	36.0	31.0	39.0	22.0	40.0
37	33.27152968790113	36.0	31.0	39.0	22.0	40.0
38	33.087116294421826	36.0	31.0	39.0	21.0	40.0
39	32.780709282282615	35.0	31.0	39.0	20.0	40.0
40	32.16113250449261	35.0	30.0	39.0	19.0	40.0
41	32.16402244471339	35.0	30.0	38.0	19.0	40.0
42	31.883888950012835	35.0	29.0	38.0	18.0	40.0
43	30.600535445776945	34.0	27.0	37.0	14.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	18.0
11	12.0
12	13.0
13	10.0
14	4.0
15	9.0
16	9.0
17	18.0
18	42.0
19	67.0
20	145.0
21	266.0
22	410.0
23	745.0
24	1141.0
25	1652.0
26	2402.0
27	3245.0
28	4280.0
29	5230.0
30	6515.0
31	7985.0
32	9419.0
33	11344.0
34	13574.0
35	15616.0
36	16966.0
37	16133.0
38	14171.0
39	4883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.025048593537974	24.763266952726738	20.047676678769207	24.164007774966077
2	12.624051050720652	22.36036234275865	40.24791872959989	24.76766787692082
3	15.245534895661422	31.43433454358749	34.83478197088055	18.48534858987054
4	9.749514064620236	18.720064546888178	26.130487402354497	45.399933986137086
5	13.928925074265596	38.68705761543257	27.15150181538123	20.232515494920598
6	26.140756225474014	35.151648512854365	15.133311328712363	23.574283932959254
7	21.210254153372208	36.354567792569775	15.246268383027104	27.188909671030913
8	29.084241023948366	25.82829060769428	25.23049840466498	19.856969963692375
9	19.01419298052591	11.173213041405363	14.081490446327061	55.73110353174167
10	15.418637913961932	24.24175743572817	27.846114350680313	32.49349029962959
11	29.19866505299446	19.607584259361133	19.921516851872227	31.272233835772177
12	17.830344372318187	28.877397586826564	20.476766787692082	32.81549125316317
13	37.77826676935489	14.982212931382257	21.28360288994022	25.955917409322627
14	23.641031283236146	27.38328382293615	22.21146440752558	26.764220486302126
15	30.396449921150108	25.765944181611474	18.101734697619833	25.735871199618586
16	27.95833791762937	22.554736494663878	17.317636703707777	32.16928888399897
17	17.869952690064913	33.25411669783988	18.456009095243335	30.419921516851872
18	23.8346719477757	15.371694722558404	21.367953936993437	39.425679392672464
19	26.51116734514248	25.454945538563102	20.968936810063447	27.064950306230973
20	26.197968239997067	22.007554919866507	21.717093923057178	30.077382917079255
21	32.36439652326989	23.741519052334322	21.444236623024167	22.44984780137162
22	29.521399493893718	31.55902739575311	21.296805662522463	17.62276744783071
23	32.49202332489823	18.4941504382587	21.93053874647009	27.083287490372975
24	24.19188029486192	23.90435324751531	25.126343198738404	26.777423258884365
25	26.643928558330582	22.73003997506143	28.589870539479957	22.03616092712803
26	28.819452084937836	24.374518648916272	21.57993178567499	25.226097480470898
27	29.046099680932997	26.720211244361312	27.52631385924377	16.707375215461916
28	21.381156709575677	24.59236439652327	29.020427623134193	25.00605127076686
29	25.248102101441305	18.84329042432244	24.393589320423956	31.515018153812303
30	28.625811420398286	18.121538856493196	34.7959071404995	18.456742582609014
31	27.200645468881802	19.788022151318444	21.260864781604134	31.75046759819562
32	26.544907763963764	20.11515751641178	35.420838376058974	17.91909634356548
33	27.67007738291708	19.466754685150548	33.506436351633845	19.35673158029853
34	17.108592804488943	30.41111966846371	26.87791102798254	25.602376499064803
35	19.694869255877066	18.131074192247038	45.395533061943006	16.778523489932887
36	27.78230094986614	19.466754685150548	22.67796237209814	30.072981992885172
37	21.144240290460996	21.16037701250596	31.093262918546227	26.602119778486816
38	21.467708218725935	17.004437598562365	28.568599405875233	32.95925477683647
39	31.117468001613673	18.802215131844356	26.22070634833315	23.859610518208825
40	17.38658451608171	17.823009498661385	41.62540800234716	23.164997982909743
41	24.14126966662999	22.3214875123776	24.68478380459897	28.852459016393446
42	20.338137675578537	17.298566032200096	40.53177834011809	21.831517952103276
43	25.06473026002127	17.55015219862838	31.515018153812303	25.870099387538048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	6.0
2	10.0
3	25.5
4	41.0
5	41.0
6	102.0
7	163.0
8	187.5
9	212.0
10	289.5
11	367.0
12	367.0
13	631.0
14	895.0
15	2149.0
16	3403.0
17	3309.5
18	3216.0
19	3216.0
20	2737.0
21	2258.0
22	1730.5
23	1203.0
24	1101.5
25	1000.0
26	1000.0
27	980.0
28	960.0
29	907.5
30	855.0
31	880.5
32	906.0
33	906.0
34	1078.0
35	1250.0
36	1242.0
37	1234.0
38	1349.0
39	1464.0
40	1464.0
41	1904.0
42	2344.0
43	3134.0
44	3924.0
45	5328.5
46	6733.0
47	6733.0
48	8543.5
49	10354.0
50	10131.0
51	9908.0
52	11611.0
53	13314.0
54	13314.0
55	19104.0
56	24894.0
57	17052.0
58	9210.0
59	14545.5
60	19881.0
61	19881.0
62	15970.5
63	12060.0
64	7132.5
65	2205.0
66	1677.0
67	1149.0
68	1149.0
69	851.0
70	553.0
71	396.5
72	240.0
73	156.0
74	72.0
75	72.0
76	50.5
77	29.0
78	21.5
79	14.0
80	11.5
81	9.0
82	9.0
83	6.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.948105768878133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.74712609884456	19.146954193714013
2	9.540469820362803	4.7603330032640185
3	3.6838855731632023	2.7571790075915943
4	2.184458883368124	2.1799244508013347
5	1.458265957134037	1.8190486668867127
6	0.9319965895392938	1.3950929695236
7	0.7114926645694294	1.2425275974621337
8	0.5997706759180313	1.1970513807899659
9	0.4057272219445506	0.9109913081747167
>10	3.107635315908623	15.533795430373711
>50	0.3616264369505777	6.192100341071625
>100	0.22050392496986448	10.210144130267357
>500	0.020580366330520683	3.8038654784171344
>1k	0.020580366330520683	15.63575017420325
>5k	0.005880104665863053	13.215241867458833
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	9263	6.794293468295008	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	8754	6.420948399163824	TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	4689	3.4393222576741116	No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	4595	3.37037444530018	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	4261	3.1253896651630177	RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2747	2.0148897935233068	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2213	1.6232075402501192	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1785	1.309274947739025	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1027	0.7532915245534896	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	905	0.6638060659405142	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	852	0.6249312355594675	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	831	0.6095280008801849	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	801	0.587523379909781	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	617	0.45256170462463785	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	596	0.4371584699453552	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	584	0.4283566215571937	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	491	0.3601422965489419	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	423	0.31026515568269336	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	404	0.29632889573477095	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	392	0.28752704734660944	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	371	0.2721238126673268	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	368	0.26992335057028644	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.23178200755491987	No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT	304	0.22298015916675834	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	296	0.21711226024131733	No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	288	0.21124436131587632	TruSeq Adapter, Index 10 (95% over 23bp)
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	285	0.20904389921883598	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	275	0.20170902556203468	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	271	0.19877507609931416	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	254	0.18630579088275204	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	237	0.1738365056661899	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	229	0.1679686067407489	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	218	0.1599002457182675	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	214	0.15696629625554698	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	211	0.15476583415850662	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	210	0.15403234679282649	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	210	0.15403234679282649	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	209	0.15329885942714638	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	200	0.14669747313602524	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	191	0.1400960868449041	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	190	0.13936259947922397	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	189	0.13862911211354384	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	184	0.13496167528514322	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	181	0.13276121318810283	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	179	0.13129423845674257	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	177	0.12982726372538234	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	172	0.12615982689698169	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	171	0.12542633953130156	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	171	0.12542633953130156	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	168	0.1232258774342612	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.12175890270290095	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	159	0.11662449114314005	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	157	0.1151575164117798	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	155	0.11369054168041955	No Hit
CTGATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	154	0.11295705431473943	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	153	0.1122235669490593	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	152	0.11149007958337917	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	149	0.1092896174863388	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	148	0.10855613012065866	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	147	0.10782264275497856	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	145	0.10635566802361829	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	144	0.10562218065793816	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	140	0.10268823119521765	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.334873656801262E-4	0.0
9	0.0	0.0	0.0	7.334873656801262E-4	0.0
10	0.0	0.0	0.0	7.334873656801262E-4	0.0
11	0.0	0.0	0.0	7.334873656801262E-4	0.0
12	0.0	0.0	0.0	7.334873656801262E-4	0.0
13	0.0	0.0	0.0	7.334873656801262E-4	0.0
14	0.0	0.0	0.0	7.334873656801262E-4	0.0
15	0.0	0.0	0.0	7.334873656801262E-4	0.0
16	0.0	0.0	0.0	7.334873656801262E-4	0.0
17	0.0	0.0	0.0	7.334873656801262E-4	0.0
18	0.0	0.0	0.0	0.0014669747313602523	0.0
19	0.0	0.0	0.0	0.0014669747313602523	0.0
20	0.0	0.0	0.0	0.0022004620970403786	0.0
21	0.0	0.0	0.0	0.0022004620970403786	0.0
22	0.0	0.0	0.0	0.0022004620970403786	0.0
23	0.0	0.0	0.0	0.0022004620970403786	0.0
24	0.0	0.0	0.0	0.004400924194080757	0.0
25	0.0	0.0	0.0	0.005134411559760883	0.0
26	0.0	0.0	0.0	0.006601386291121136	0.0
27	0.0	0.0	0.0	0.008068361022481388	0.0
28	0.0	0.0	0.0	0.016870209410642902	0.0
29	0.0	0.0	0.0	0.04107529247808706	0.0
30	0.0	0.0	0.0	0.06748083764257161	0.0
31	0.0	0.0	0.0	0.11295705431473943	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACACCG	25	1.227925E-4	37.0	5
CTTATAC	545	0.0	35.6422	1
CGTCTTC	560	0.0	33.36607	37
TTTACAC	35	2.3730086E-5	31.714285	2
GTCCGGC	30	3.5830028E-4	30.833332	23
GCGGTCC	30	3.5830028E-4	30.833332	20
CGGTCCG	30	3.5830028E-4	30.833332	21
TTATACA	645	0.0	30.116278	2
GTATCTA	25	0.0054795714	29.6	3
CTCAGAG	25	0.0054795714	29.6	2
GGGAACC	25	0.0054795714	29.6	18
GGTGCTT	25	0.0054795714	29.6	33
GGTATCA	320	0.0	26.593752	1
CTATACA	35	8.8291813E-4	26.42857	1
GGTCCGG	35	8.8291813E-4	26.42857	22
AACTCAA	35	8.8291813E-4	26.42857	34
TCCGGCC	35	8.8291813E-4	26.42857	24
TATACAC	760	0.0	26.046053	3
TATGTGC	45	1.3148715E-4	24.666666	4
GTCTTCT	60	1.3230147E-6	24.666666	37
>>END_MODULE