FastQCFastQC Report
Fri 10 Feb 2017
ERR1631089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977538
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT172791.7676039192338302No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG139711.429202752220374No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT91480.935820397774818TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC77990.797820647381483No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA43570.44571157336083095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37710.3857650546577217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.3676583416706051No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC34040.34822175710816355No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC27950.2859223886948641RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG18590.19017163527146771No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13910.14229625855976955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13510.13820434602030818No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA13150.13452162473479293No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA12140.12418954557265292No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC11310.11569882705327057No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT10500.10741270416086127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21400.032.764021
GATTACG658.731149E-1131.3076921
TATACAC23800.029.8487383
TTATACA23450.029.7420042
CGCGACT250.00549594629.623
GCTTCGT250.00549594629.635
GGTATCA6750.029.3259261
CCGTCTT13950.029.30824337
GCCGTCT14750.027.84406936
CGTCTTC3200.026.5937537
GTATCAA20450.024.606361
GCTTTAT6900.023.862321
TTACGGG903.8271537E-922.6111133
TGCCGTC18300.022.34153235
CGACACG502.7017013E-422.221
ATGCCGT19200.021.2942734
AGGACCG705.1006355E-621.1428575
ATTGAGC7550.021.072856
TAGGACC1850.021.04
TATGCCG21100.019.20142233
Produced by FastQC (version 0.11.5)