##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	977538
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307175782424828	31.0	31.0	34.0	28.0	34.0
2	31.295435062370977	31.0	31.0	34.0	28.0	34.0
3	30.746858945636895	31.0	31.0	34.0	26.0	34.0
4	34.94164523527474	37.0	35.0	37.0	32.0	37.0
5	35.13146803500222	37.0	35.0	37.0	32.0	37.0
6	35.33977195771418	37.0	35.0	37.0	33.0	37.0
7	35.349609938437176	37.0	35.0	37.0	33.0	37.0
8	35.37745335731194	37.0	35.0	37.0	33.0	37.0
9	37.12895969261553	39.0	37.0	39.0	33.0	39.0
10	36.94775446069616	39.0	37.0	39.0	33.0	39.0
11	37.00652864645671	39.0	37.0	39.0	33.0	39.0
12	36.9287986758571	39.0	37.0	39.0	33.0	39.0
13	37.01730367515125	39.0	37.0	39.0	33.0	39.0
14	38.088540803528865	40.0	37.0	41.0	33.0	41.0
15	38.09717780792153	40.0	37.0	41.0	33.0	41.0
16	38.09099390509627	40.0	37.0	41.0	33.0	41.0
17	38.072785917273805	40.0	37.0	41.0	33.0	41.0
18	38.12204845233638	40.0	37.0	41.0	33.0	41.0
19	38.14697638352678	40.0	37.0	41.0	33.0	41.0
20	38.1561402216589	40.0	37.0	41.0	33.0	41.0
21	38.10358676593646	40.0	37.0	41.0	33.0	41.0
22	38.00234159695071	40.0	37.0	41.0	33.0	41.0
23	38.03666251337544	40.0	37.0	41.0	33.0	41.0
24	37.99519711765681	40.0	37.0	41.0	33.0	41.0
25	37.96095190161405	40.0	37.0	41.0	33.0	41.0
26	37.85635545625848	40.0	37.0	41.0	33.0	41.0
27	37.75232369483334	40.0	37.0	41.0	33.0	41.0
28	37.75464994711203	40.0	37.0	41.0	33.0	41.0
29	37.67923293007535	39.0	37.0	41.0	32.0	41.0
30	37.586803786655864	39.0	37.0	41.0	32.0	41.0
31	37.567260812367394	39.0	37.0	41.0	32.0	41.0
32	37.4630101336214	39.0	36.0	41.0	32.0	41.0
33	37.43801366289597	39.0	36.0	41.0	32.0	41.0
34	37.39078378538737	39.0	36.0	41.0	32.0	41.0
35	37.360005442243676	39.0	36.0	41.0	32.0	41.0
36	37.32936417817005	39.0	36.0	41.0	31.0	41.0
37	37.250146797362355	39.0	36.0	41.0	31.0	41.0
38	37.20375780788061	39.0	36.0	41.0	31.0	41.0
39	37.11955545462171	39.0	36.0	40.0	31.0	41.0
40	37.024540222477285	39.0	36.0	40.0	31.0	41.0
41	36.97723464458671	39.0	36.0	40.0	31.0	41.0
42	36.904215488298156	39.0	36.0	40.0	31.0	41.0
43	36.19844650540439	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	15.0
11	20.0
12	12.0
13	14.0
14	8.0
15	10.0
16	11.0
17	9.0
18	21.0
19	39.0
20	105.0
21	219.0
22	430.0
23	838.0
24	1440.0
25	2435.0
26	4039.0
27	6052.0
28	9016.0
29	12611.0
30	17639.0
31	23738.0
32	30232.0
33	39632.0
34	52100.0
35	70050.0
36	96592.0
37	144244.0
38	244565.0
39	221394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75726570220288	22.181541791725742	15.282372654566881	23.778819851504494
2	13.941248319758415	22.3224058808967	40.46297944427736	23.273366355067527
3	15.00371341062956	28.675100098410493	34.24562523400625	22.075561256953694
4	11.658063420552448	19.092761611313318	38.67276770826301	30.57640725987123
5	12.488926261690082	38.211097675998275	35.968934200000405	13.331041862311235
6	27.40630031773701	40.743480048857435	17.362905585256023	14.487314048149535
7	24.388617117697724	32.980303578991304	22.97598661126217	19.6550926920488
8	25.11124887216661	34.39395706356173	22.50510977578365	17.989684288488018
9	24.436492494409425	15.070309287209296	19.572026867497737	40.92117135088355
10	14.69937741550712	29.272621626985345	33.254768612575674	22.77323234493186
11	31.843570275528933	23.439907195423604	25.153190975696088	19.56333155335138
12	21.40520368517643	26.374626868725308	28.705175655575538	23.51499379052272
13	29.292978891869165	20.83653014000479	26.680088139796098	23.190402828329947
14	21.575631842445002	22.134280201894967	27.24630653744407	29.043781418215968
15	23.963467404847687	28.961124784918844	23.010358676593647	24.065049133639818
16	24.028528814225126	27.31760811344418	24.257266725181015	24.396596347149675
17	21.56437908296148	29.997503933350927	25.065624047351616	23.372492936335977
18	23.98934875165978	25.45681088612412	26.916396088949995	23.6374442732661
19	24.48620923176388	26.888571083681654	27.25807078599502	21.367148898559442
20	23.931345891412917	26.568072034028344	25.63122865811866	23.869353416440077
21	25.364538258359264	25.33231444711101	26.325728513878744	22.977418780650982
22	21.568470995500945	30.19135828990791	26.50423819841275	21.735932516178398
23	24.487027614271774	25.211909920637353	26.440199767170174	23.860862697920695
24	22.77118638866213	26.97194380167318	27.350752605013824	22.906117204650865
25	22.193306040276696	28.79581151832461	26.710163696961143	22.300718744437557
26	24.607636736372395	25.93300720790394	27.191168015974824	22.268188039748836
27	21.72345218293304	29.558646313493696	26.668119295618176	22.049782207955086
28	23.702710278270512	25.556755849900465	27.280985496216005	23.45954837561302
29	21.694501901716354	26.40664608434659	28.045047865146934	23.853804148790122
30	23.719487119682302	27.312902414023803	27.558826357645433	21.40878410864846
31	23.886027960038383	25.403718320924607	25.950807027450594	24.759446691586415
32	21.88262758071809	26.506795643749907	28.39163285723931	23.218943918292688
33	22.542448477706237	25.99438589599586	28.36063661975289	23.102529006545012
34	22.11873093424501	27.14871442337791	26.75210580049062	23.980448841886453
35	21.205211459810258	25.627341341206172	31.509567914495396	21.657879284488175
36	22.33191957755095	26.086658523760715	26.399485237402537	25.181936661285803
37	22.432171434767753	26.855938081179453	28.457717244751613	22.254173239301185
38	22.362813517223884	25.16106790733455	28.765429067719104	23.710689507722464
39	23.996100407349893	25.725547242153247	26.922636255572673	23.355716094924187
40	21.50177282110772	25.017544075012943	31.335661631568286	22.14502147231105
41	22.280975266434655	25.896486888489246	26.626790978969616	25.195746866106482
42	22.732722410791194	25.154111656017463	29.25011610801831	22.863049825173036
43	23.041457211893555	25.18694925414664	29.195693671243472	22.575899862716334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	32.5
2	47.0
3	139.0
4	231.0
5	231.0
6	305.0
7	379.0
8	372.5
9	366.0
10	589.0
11	812.0
12	812.0
13	1204.5
14	1597.0
15	3769.0
16	5941.0
17	6708.0
18	7475.0
19	7475.0
20	7804.0
21	8133.0
22	9850.5
23	11568.0
24	14803.0
25	18038.0
26	18038.0
27	22124.5
28	26211.0
29	30360.0
30	34509.0
31	38830.0
32	43151.0
33	43151.0
34	47008.5
35	50866.0
36	53914.0
37	56962.0
38	58468.0
39	59974.0
40	59974.0
41	61386.0
42	62798.0
43	63382.5
44	63967.0
45	64787.0
46	65607.0
47	65607.0
48	66414.0
49	67221.0
50	66586.5
51	65952.0
52	78636.0
53	91320.0
54	91320.0
55	73444.0
56	55568.0
57	57949.5
58	60331.0
59	51479.5
60	42628.0
61	42628.0
62	34890.5
63	27153.0
64	23353.0
65	19553.0
66	16002.0
67	12451.0
68	12451.0
69	10288.5
70	8126.0
71	6812.5
72	5499.0
73	3614.5
74	1730.0
75	1730.0
76	1214.5
77	699.0
78	543.0
79	387.0
80	264.5
81	142.0
82	142.0
83	108.5
84	75.0
85	54.0
86	33.0
87	23.5
88	14.0
89	14.0
90	9.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	977538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.95680080033892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.58998218879493	40.046940672966066
2	12.899271245250695	13.14611190627559
3	3.8152002044268087	5.832311904911675
4	1.47209812451995	3.000536435588624
5	0.7706740844686105	1.9635542902125276
6	0.4718986084370004	1.4427866032848817
7	0.3011659022528155	1.0742515622265743
8	0.2134211129206843	0.8700205710148758
9	0.16123537788746634	0.7394435129681093
>10	1.0912667491397279	10.99668788715729
>50	0.12486193585840809	4.416209740287773
>100	0.08209956349575456	7.526775927344359
>500	0.003613183701407648	1.2289761832021355
>1k	0.002408789134271765	2.7854747749446984
>5k	4.014648557119609E-4	1.7334547962319262
>10k+	4.014648557119609E-4	3.196463231382999
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	17279	1.7676039192338302	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	13971	1.429202752220374	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	9148	0.935820397774818	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	7799	0.797820647381483	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4357	0.44571157336083095	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3771	0.3857650546577217	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3594	0.3676583416706051	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	3404	0.34822175710816355	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2795	0.2859223886948641	RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1859	0.19017163527146771	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1391	0.14229625855976955	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.13820434602030818	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1315	0.13452162473479293	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1214	0.12418954557265292	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1131	0.11569882705327057	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	1050	0.10741270416086127	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.0229781348653454E-4	0.0
9	0.0	0.0	0.0	1.0229781348653454E-4	0.0
10	0.0	0.0	0.0	1.0229781348653454E-4	0.0
11	0.0	0.0	0.0	1.0229781348653454E-4	0.0
12	0.0	0.0	0.0	1.0229781348653454E-4	0.0
13	0.0	0.0	0.0	1.0229781348653454E-4	0.0
14	0.0	0.0	0.0	2.0459562697306907E-4	0.0
15	0.0	0.0	0.0	2.0459562697306907E-4	0.0
16	0.0	0.0	0.0	3.068934404596036E-4	0.0
17	0.0	0.0	0.0	3.068934404596036E-4	0.0
18	0.0	0.0	0.0	4.0919125394613814E-4	0.0
19	0.0	0.0	0.0	5.114890674326727E-4	0.0
20	0.0	0.0	0.0	5.114890674326727E-4	0.0
21	0.0	0.0	0.0	7.160846944057418E-4	0.0
22	0.0	0.0	0.0	0.00112527594835188	0.0
23	0.0	0.0	0.0	0.0016367650157845526	0.0
24	0.0	0.0	0.0	0.002659743150649898	0.0
25	0.0	0.0	0.0	0.002864338777622967	0.0
26	0.0	0.0	0.0	0.003068934404596036	0.0
27	0.0	0.0	0.0	0.003785019099001778	0.0
28	0.0	0.0	0.0	0.00992288790819385	0.0
29	0.0	0.0	0.0	0.02567675118512017	0.0
30	0.0	0.0	0.0	0.046034016068940545	0.0
31	0.0	0.0	0.0	0.09728522062569435	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2140	0.0	32.76402	1
GATTACG	65	8.731149E-11	31.307692	1
TATACAC	2380	0.0	29.848738	3
TTATACA	2345	0.0	29.742004	2
CGCGACT	25	0.005495946	29.6	23
GCTTCGT	25	0.005495946	29.6	35
GGTATCA	675	0.0	29.325926	1
CCGTCTT	1395	0.0	29.308243	37
GCCGTCT	1475	0.0	27.844069	36
CGTCTTC	320	0.0	26.59375	37
GTATCAA	2045	0.0	24.60636	1
GCTTTAT	690	0.0	23.86232	1
TTACGGG	90	3.8271537E-9	22.611113	3
TGCCGTC	1830	0.0	22.341532	35
CGACACG	50	2.7017013E-4	22.2	21
ATGCCGT	1920	0.0	21.29427	34
AGGACCG	70	5.1006355E-6	21.142857	5
ATTGAGC	755	0.0	21.07285	6
TAGGACC	185	0.0	21.0	4
TATGCCG	2110	0.0	19.201422	33
>>END_MODULE