FastQCFastQC Report
Fri 10 Feb 2017
ERR1631088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135097
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT1782613.194963618733208No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1588811.760438795828183RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG1459110.800387869456761No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC74095.484207643396967No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC68785.091156724427633RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC45763.387195866673575RNA PCR Primer, Index 39 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA16121.1932167257600095No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG15241.1280783437085946No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT13230.9792963574320672No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9780.723924291435043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.666187998253107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8510.6299177627926601No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT7540.5581175007587141Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT5000.3701044434739484Illumina PCR Primer Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3510.2598133193187117No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2840.21021932389320266No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT2760.2042976527976195No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2390.1769099239805473RNA PCR Primer, Index 39 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA2290.16950783511106834No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2250.16654699956327676No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2210.16358616401548515No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1820.1347180174245172No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA1690.12509530189419454No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1550.11473237747692398No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC1400.10362924417270554No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1380.10214882639880975No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCC200.001836191337.07
AACGTCA200.001836191337.028
TAAACAT200.001836191337.02
GTCAATG251.2278651E-437.031
TTATTAC200.001836191337.02
TTCTGCT200.001836191337.037
TTCTGCG200.001836191337.018
TCAATGA251.2278651E-437.032
ATTACAC200.001836191337.04
TCTGCGG200.001836191337.019
CTGCGGG200.001836191337.020
TCGCCGA308.257746E-637.01
TATTACA200.001836191337.03
TTTACAC251.2278651E-437.02
TGAGCAA200.001836191337.036
AATGAGC200.001836191337.034
CTTATAC19550.036.4322281
TTATACA20950.033.9093062
TGATACA451.06800144E-732.888892
CGTCTTC7950.032.3459137
Produced by FastQC (version 0.11.5)