##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135097
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90265512927748	31.0	30.0	34.0	27.0	34.0
2	31.104961620169213	31.0	31.0	34.0	28.0	34.0
3	30.600694315935957	31.0	30.0	34.0	26.0	34.0
4	34.63959969503394	35.0	35.0	37.0	32.0	37.0
5	34.79372598947423	35.0	35.0	37.0	32.0	37.0
6	34.95913306735161	35.0	35.0	37.0	32.0	37.0
7	34.87413488086338	35.0	35.0	37.0	32.0	37.0
8	34.95684582189094	35.0	35.0	37.0	32.0	37.0
9	36.83790165584728	39.0	37.0	39.0	33.0	39.0
10	36.66062162742326	38.0	35.0	39.0	32.0	39.0
11	36.63147960354412	38.0	35.0	39.0	32.0	39.0
12	36.49289029364087	38.0	35.0	39.0	32.0	39.0
13	36.67926008719661	39.0	35.0	39.0	32.0	39.0
14	37.27819270598163	39.0	36.0	40.0	31.0	41.0
15	37.209464310828515	39.0	36.0	40.0	31.0	41.0
16	37.135732103599636	39.0	36.0	40.0	31.0	41.0
17	37.00655084864949	39.0	36.0	40.0	31.0	41.0
18	37.380689430557304	39.0	36.0	40.0	32.0	41.0
19	37.296409246689414	39.0	36.0	40.0	31.0	41.0
20	37.454651102541135	39.0	36.0	40.0	32.0	41.0
21	37.40388757707425	39.0	36.0	40.0	32.0	41.0
22	37.08097145014323	39.0	36.0	40.0	31.0	41.0
23	37.28334455983479	39.0	36.0	40.0	31.0	41.0
24	36.9548916704294	39.0	36.0	40.0	31.0	41.0
25	36.81069157716308	38.0	35.0	40.0	30.0	41.0
26	36.684249095094636	39.0	35.0	40.0	30.0	41.0
27	36.41375456153727	38.0	35.0	40.0	30.0	41.0
28	36.76413984026292	39.0	35.0	40.0	30.0	41.0
29	36.69022998290117	39.0	35.0	40.0	30.0	41.0
30	36.64288622249199	38.0	35.0	40.0	30.0	41.0
31	36.638200700237604	39.0	35.0	40.0	30.0	41.0
32	36.19930124281072	38.0	35.0	40.0	30.0	41.0
33	36.00231685381615	38.0	35.0	40.0	30.0	41.0
34	35.937733628429946	38.0	35.0	40.0	30.0	41.0
35	36.0048261619429	38.0	35.0	40.0	30.0	41.0
36	36.028098329348545	38.0	35.0	40.0	30.0	41.0
37	35.9648104695145	38.0	35.0	40.0	29.0	41.0
38	35.929480299340476	38.0	35.0	40.0	30.0	41.0
39	35.546725685988584	38.0	34.0	40.0	27.0	41.0
40	35.01526310724886	38.0	33.0	40.0	26.0	41.0
41	35.18457108596046	38.0	33.0	40.0	26.0	41.0
42	35.05973485717669	38.0	33.0	40.0	26.0	41.0
43	33.79730860048706	37.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	10.0
11	21.0
12	19.0
13	25.0
14	15.0
15	9.0
16	5.0
17	19.0
18	22.0
19	30.0
20	34.0
21	79.0
22	136.0
23	222.0
24	433.0
25	674.0
26	1050.0
27	1559.0
28	2286.0
29	3092.0
30	4041.0
31	4838.0
32	6142.0
33	7542.0
34	9285.0
35	11519.0
36	15119.0
37	19974.0
38	27918.0
39	18963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.977453237303568	26.107907651539264	21.81913736056315	34.09550175059402
2	7.008297741622687	16.092141202247273	48.48812334840893	28.41143770772112
3	8.749269043724139	30.666854186251356	44.64347838960154	15.940398380422954
4	5.6766619540034196	24.80958126383265	17.4385811676055	52.07517561455843
5	11.614617645099447	34.84755397973308	31.75348083229087	21.7843475428766
6	24.54014522898362	36.77209708579761	13.139447952212112	25.54830973300665
7	16.23426130854127	38.507886925690435	9.747810832216853	35.510040933551444
8	27.67567007409491	28.199737966054023	25.632693546118713	18.491898413732354
9	11.92328475095672	10.465073243669364	9.091985758381014	68.5196562469929
10	12.060963603929029	27.03390896911108	24.66376011310392	36.241367313855974
11	20.615557710385872	15.853053731763103	24.828086486006352	38.703302071844675
12	11.180855237347979	29.307830669815022	13.036558916926358	46.474755175910644
13	36.19399394509131	9.596068010392534	24.316602145125355	29.89333589939081
14	23.982767937111856	29.43662701614396	11.925505377617563	34.65509966912663
15	30.04729934787597	25.720038194778567	9.788522320998986	34.444140136346476
16	40.81067677298534	18.846458470580398	9.947667231692783	30.39519752474148
17	12.223069350170618	46.95441053465288	10.33997794177517	30.482542173401335
18	29.144244505799538	10.439165932626187	11.9306868398262	48.485902721748076
19	17.572558976143068	25.628252292797026	17.263891870285793	39.53529686077411
20	11.720467515932995	16.19947149085472	24.86065567703206	47.21940531618022
21	27.324811061681608	12.406641154133697	17.51630310073503	42.75224468344967
22	10.479877421408322	41.932093236711395	28.47731629865948	19.110713043220798
23	22.719231367091794	11.318534090320288	18.602929746774542	47.35930479581338
24	24.768129566163573	27.922899842335507	29.636483415619885	17.67248717588103
25	11.00468552225438	39.22736996380379	18.92344019482298	30.844504319118855
26	23.880619110713045	17.6488004914987	17.246867065885993	41.22371333190226
27	9.405834326446923	40.38579687187725	36.09258532757944	14.115783474096391
28	8.539049719830937	11.274121557103415	27.726004278407366	52.460824444658286
29	9.194874793666772	14.477005410926964	36.713620583728726	39.61449921167753
30	9.51242440616742	16.305321361688268	44.06611545778218	30.11613877436212
31	15.990732584735412	10.2060001332376	26.854778418469692	46.948488863557294
32	20.9220041895823	27.983596971065232	37.99492216703554	13.099476672316928
33	9.610131979244544	16.31494407721859	33.74538294706767	40.329540996469206
34	12.605757344722681	35.740986106279195	33.63879286734716	18.01446368165096
35	15.095079831528457	26.206355433503337	45.548013649451875	13.150551085516332
36	23.244779676824802	15.217954506761808	14.095057625261848	47.44220819115154
37	16.440039379112786	25.31366351584417	29.307830669815022	28.938466435228023
38	18.465991102689177	11.106834348653189	33.94153830210885	36.48563624654877
39	34.519641442815164	15.05880959606801	20.628881470350933	29.792667490765893
40	11.955853941982427	10.145303004507872	56.383931545482135	21.514911508027566
41	20.962715678364436	20.61259687483808	19.740630806013456	38.68405664078403
42	24.375818856081185	10.999504060045744	46.437004522676304	18.18767256119677
43	22.34986713250479	11.345921819137361	41.08529427004301	25.218916778314842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	18.5
4	36.0
5	36.0
6	60.5
7	85.0
8	91.0
9	97.0
10	140.0
11	183.0
12	183.0
13	300.5
14	418.0
15	899.5
16	1381.0
17	1411.0
18	1441.0
19	1441.0
20	1197.0
21	953.0
22	781.0
23	609.0
24	620.0
25	631.0
26	631.0
27	677.5
28	724.0
29	714.0
30	704.0
31	813.5
32	923.0
33	923.0
34	985.5
35	1048.0
36	1097.5
37	1147.0
38	1238.5
39	1330.0
40	1330.0
41	1535.5
42	1741.0
43	2017.5
44	2294.0
45	2996.5
46	3699.0
47	3699.0
48	6485.5
49	9272.0
50	24632.0
51	39992.0
52	24149.5
53	8307.0
54	8307.0
55	20164.0
56	32021.0
57	25317.5
58	18614.0
59	10895.5
60	3177.0
61	3177.0
62	2477.0
63	1777.0
64	1404.5
65	1032.0
66	864.0
67	696.0
68	696.0
69	541.5
70	387.0
71	294.5
72	202.0
73	141.5
74	81.0
75	81.0
76	61.5
77	42.0
78	36.0
79	30.0
80	22.0
81	14.0
82	14.0
83	8.5
84	3.0
85	4.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	135097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.86718431941494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.33139785789949	14.590257370629992
2	9.274589038408726	3.4997076174896558
3	3.7624073129585307	2.129580967749099
4	1.9694770293067598	1.4863394449913767
5	1.3260622229196908	1.2509530189419453
6	0.9965082976970457	1.1280783437085946
7	0.8121150300129467	1.0725626771875023
8	0.6237985013142925	0.9415457041977247
9	0.48256110479030173	0.8194112378513217
>10	3.048373808309467	11.249694663834134
>50	0.2275491388442073	2.9919243210433986
>100	0.09415826434932717	3.2428551337187352
>500	0.015693044058221195	2.578147553239524
>1k	0.015693044058221195	6.687787293574246
>5k	0.007846522029110597	10.5753643678246
>10k+	0.011769783043665896	35.755790284018154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	17826	13.194963618733208	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	15888	11.760438795828183	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	14591	10.800387869456761	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	7409	5.484207643396967	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	6878	5.091156724427633	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	4576	3.387195866673575	RNA PCR Primer, Index 39 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1612	1.1932167257600095	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1524	1.1280783437085946	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1323	0.9792963574320672	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	978	0.723924291435043	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.666187998253107	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	851	0.6299177627926601	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	754	0.5581175007587141	Illumina PCR Primer Index 11 (95% over 24bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	500	0.3701044434739484	Illumina PCR Primer Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	351	0.2598133193187117	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	284	0.21021932389320266	No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	276	0.2042976527976195	No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	239	0.1769099239805473	RNA PCR Primer, Index 39 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	229	0.16950783511106834	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	225	0.16654699956327676	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	221	0.16358616401548515	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	182	0.1347180174245172	No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	169	0.12509530189419454	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	155	0.11473237747692398	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	140	0.10362924417270554	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	138	0.10214882639880975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.402088869478966E-4	0.0
9	0.0	0.0	0.0	0.0014804177738957933	0.0
10	0.0	0.0	0.0	0.00222062666084369	0.0
11	0.0	0.0	0.0	0.00222062666084369	0.0
12	0.0	0.0	0.0	0.00222062666084369	0.0
13	0.0	0.0	0.0	0.00222062666084369	0.0
14	0.0	0.0	0.0	0.00222062666084369	0.0
15	0.0	0.0	0.0	0.0029608355477915866	0.0
16	0.0	0.0	0.0	0.0029608355477915866	0.0
17	0.0	0.0	0.0	0.0029608355477915866	0.0
18	0.0	0.0	0.0	0.0037010444347394837	0.0
19	0.0	0.0	0.0	0.0037010444347394837	0.0
20	0.0	0.0	0.0	0.00444125332168738	0.0
21	0.0	0.0	0.0	0.005181462208635277	0.0
22	0.0	0.0	0.0	0.007402088869478967	0.0
23	0.0	0.0	0.0	0.010362924417270554	0.0
24	0.0	0.0	0.0	0.011843342191166346	0.0
25	0.0	0.0	0.0	0.012583551078114244	0.0
26	0.0	0.0	0.0	0.01332375996506214	0.0
27	0.0	0.0	0.0	0.01776501328674952	0.0
28	0.0	0.0	0.0	0.02664751993012428	0.0
29	0.0	0.0	0.0	0.043672324329925905	0.0
30	0.0	0.0	0.0	0.07254047092089387	0.0
31	0.0	0.0	0.0	0.10214882639880975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTCC	20	0.0018361913	37.0	7
AACGTCA	20	0.0018361913	37.0	28
TAAACAT	20	0.0018361913	37.0	2
GTCAATG	25	1.2278651E-4	37.0	31
TTATTAC	20	0.0018361913	37.0	2
TTCTGCT	20	0.0018361913	37.0	37
TTCTGCG	20	0.0018361913	37.0	18
TCAATGA	25	1.2278651E-4	37.0	32
ATTACAC	20	0.0018361913	37.0	4
TCTGCGG	20	0.0018361913	37.0	19
CTGCGGG	20	0.0018361913	37.0	20
TCGCCGA	30	8.257746E-6	37.0	1
TATTACA	20	0.0018361913	37.0	3
TTTACAC	25	1.2278651E-4	37.0	2
TGAGCAA	20	0.0018361913	37.0	36
AATGAGC	20	0.0018361913	37.0	34
CTTATAC	1955	0.0	36.432228	1
TTATACA	2095	0.0	33.909306	2
TGATACA	45	1.06800144E-7	32.88889	2
CGTCTTC	795	0.0	32.34591	37
>>END_MODULE